全部产品

真核编辑质粒

pY016 (pcDNA3.1-hLbCpf1) CRISPR/CAS9基因编辑载体 Cas12a
发布时间:2019-02-13 20:20:26 | 浏览次数:


测序及更多信息: https://pan.baidu.com/s/1FM6iku2JAhKW3QAmE9Hzmw?pwd=hedg

采购地址:淘宝店铺地址

载体基本信息

载体名称: pY016 (pcDNA3.1-hLbCpf1)
质粒类型: 哺乳动物细胞表达载体CRISPR Cas12a
启动子: CMV
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 9227 bp
5' 测序引物:  
CMV-F: CGCAAATGGGCGGTAGGCGTG
3' 测序引物:   BGH-R:TAGAAGGCACAGTCGAGG
载体抗性:
氨苄
标签: NLS-3xHA(C端)
筛选标记: Neo/G418
备注: Expresses humanized LbCpf1
稳定性: 瞬表达
组成型: 组成型
病毒/非病毒: 非病毒



 
 全序列
1        GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG
61       CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG
121      CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC
181      TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT
241      GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA
301      TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC
361      CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC
421      ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT
481      ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT
541      ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA
601      TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG
661      ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC
721      AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG
781      GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA
841      CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC
901      GTTTAAACTT AAGCTTGGTA CCGCCACCAT GAGCAAGCTG GAGAAGTTTA CAAACTGCTA
961      CTCCCTGTCT AAGACCCTGA GGTTCAAGGC CATCCCTGTG GGCAAGACCC AGGAGAACAT
1021     CGACAATAAG CGGCTGCTGG TGGAGGACGA GAAGAGAGCC GAGGATTATA AGGGCGTGAA
1081     GAAGCTGCTG GATCGCTACT ATCTGTCTTT TATCAACGAC GTGCTGCACA GCATCAAGCT
1141     GAAGAATCTG AACAATTACA TCAGCCTGTT CCGGAAGAAA ACCAGAACCG AGAAGGAGAA
1201     TAAGGAGCTG GAGAACCTGG AGATCAATCT GCGGAAGGAG ATCGCCAAGG CCTTCAAGGG
1261     CAACGAGGGC TACAAGTCCC TGTTTAAGAA GGATATCATC GAGACAATCC TGCCAGAGTT
1321     CCTGGACGAT AAGGACGAGA TCGCCCTGGT GAACAGCTTC AATGGCTTTA CCACAGCCTT
1381     CACCGGCTTC TTTGATAACA GAGAGAATAT GTTTTCCGAG GAGGCCAAGA GCACATCCAT
1441     CGCCTTCAGG TGTATCAACG AGAATCTGAC CCGCTACATC TCTAATATGG ACATCTTCGA
1501     GAAGGTGGAC GCCATCTTTG ATAAGCACGA GGTGCAGGAG ATCAAGGAGA AGATCCTGAA
1561     CAGCGACTAT GATGTGGAGG ATTTCTTTGA GGGCGAGTTC TTTAACTTTG TGCTGACACA
1621     GGAGGGCATC GACGTGTATA ACGCCATCAT CGGCGGCTTC GTGACCGAGA GCGGCGAGAA
1681     GATCAAGGGC CTGAACGAGT ACATCAACCT GTATAATCAG AAAACCAAGC AGAAGCTGCC
1741     TAAGTTTAAG CCACTGTATA AGCAGGTGCT GAGCGATCGG GAGTCTCTGA GCTTCTACGG
1801     CGAGGGCTAT ACATCCGATG AGGAGGTGCT GGAGGTGTTT AGAAACACCC TGAACAAGAA
1861     CAGCGAGATC TTCAGCTCCA TCAAGAAGCT GGAGAAGCTG TTCAAGAATT TTGACGAGTA
1921     CTCTAGCGCC GGCATCTTTG TGAAGAACGG CCCCGCCATC AGCACAATCT CCAAGGATAT
1981     CTTCGGCGAG TGGAACGTGA TCCGGGACAA GTGGAATGCC GAGTATGACG ATATCCACCT
2041     GAAGAAGAAG GCCGTGGTGA CCGAGAAGTA CGAGGACGAT CGGAGAAAGT CCTTCAAGAA
2101     GATCGGCTCC TTTTCTCTGG AGCAGCTGCA GGAGTACGCC GACGCCGATC TGTCTGTGGT
2161     GGAGAAGCTG AAGGAGATCA TCATCCAGAA GGTGGATGAG ATCTACAAGG TGTATGGCTC
2221     CTCTGAGAAG CTGTTCGACG CCGATTTTGT GCTGGAGAAG AGCCTGAAGA AGAACGACGC
2281     CGTGGTGGCC ATCATGAAGG ACCTGCTGGA TTCTGTGAAG AGCTTCGAGA ATTACATCAA
2341     GGCCTTCTTT GGCGAGGGCA AGGAGACAAA CAGGGACGAG TCCTTCTATG GCGATTTTGT
2401     GCTGGCCTAC GACATCCTGC TGAAGGTGGA CCACATCTAC GATGCCATCC GCAATTATGT
2461     GACCCAGAAG CCCTACTCTA AGGATAAGTT CAAGCTGTAT TTTCAGAACC CTCAGTTCAT
2521     GGGCGGCTGG GACAAGGATA AGGAGACAGA CTATCGGGCC ACCATCCTGA GATACGGCTC
2581     CAAGTACTAT CTGGCCATCA TGGATAAGAA GTACGCCAAG TGCCTGCAGA AGATCGACAA
2641     GGACGATGTG AACGGCAATT ACGAGAAGAT CAACTATAAG CTGCTGCCCG GCCCTAATAA
2701     GATGCTGCCA AAGGTGTTCT TTTCTAAGAA GTGGATGGCC TACTATAACC CCAGCGAGGA
2761     CATCCAGAAG ATCTACAAGA ATGGCACATT CAAGAAGGGC GATATGTTTA ACCTGAATGA
2821     CTGTCACAAG CTGATCGACT TCTTTAAGGA TAGCATCTCC CGGTATCCAA AGTGGTCCAA
2881     TGCCTACGAT TTCAACTTTT CTGAGACAGA GAAGTATAAG GACATCGCCG GCTTTTACAG
2941     AGAGGTGGAG GAGCAGGGCT ATAAGGTGAG CTTCGAGTCT GCCAGCAAGA AGGAGGTGGA
3001     TAAGCTGGTG GAGGAGGGCA AGCTGTATAT GTTCCAGATC TATAACAAGG ACTTTTCCGA
3061     TAAGTCTCAC GGCACACCCA ATCTGCACAC CATGTACTTC AAGCTGCTGT TTGACGAGAA
3121     CAATCACGGA CAGATCAGGC TGAGCGGAGG AGCAGAGCTG TTCATGAGGC GCGCCTCCCT
3181     GAAGAAGGAG GAGCTGGTGG TGCACCCAGC CAACTCCCCT ATCGCCAACA AGAATCCAGA
3241     TAATCCCAAG AAAACCACAA CCCTGTCCTA CGACGTGTAT AAGGATAAGA GGTTTTCTGA
3301     GGACCAGTAC GAGCTGCACA TCCCAATCGC CATCAATAAG TGCCCCAAGA ACATCTTCAA
3361     GATCAATACA GAGGTGCGCG TGCTGCTGAA GCACGACGAT AACCCCTATG TGATCGGCAT
3421     CGATAGGGGC GAGCGCAATC TGCTGTATAT CGTGGTGGTG GACGGCAAGG GCAACATCGT
3481     GGAGCAGTAT TCCCTGAACG AGATCATCAA CAACTTCAAC GGCATCAGGA TCAAGACAGA
3541     TTACCACTCT CTGCTGGACA AGAAGGAGAA GGAGAGGTTC GAGGCCCGCC AGAACTGGAC
3601     CTCCATCGAG AATATCAAGG AGCTGAAGGC CGGCTATATC TCTCAGGTGG TGCACAAGAT
3661     CTGCGAGCTG GTGGAGAAGT ACGATGCCGT GATCGCCCTG GAGGACCTGA ACTCTGGCTT
3721     TAAGAATAGC CGCGTGAAGG TGGAGAAGCA GGTGTATCAG AAGTTCGAGA AGATGCTGAT
3781     CGATAAGCTG AACTACATGG TGGACAAGAA GTCTAATCCT TGTGCAACAG GCGGCGCCCT
3841     GAAGGGCTAT CAGATCACCA ATAAGTTCGA GAGCTTTAAG TCCATGTCTA CCCAGAACGG
3901     CTTCATCTTT TACATCCCTG CCTGGCTGAC ATCCAAGATC GATCCATCTA CCGGCTTTGT
3961     GAACCTGCTG AAAACCAAGT ATACCAGCAT CGCCGATTCC AAGAAGTTCA TCAGCTCCTT
4021     TGACAGGATC ATGTACGTGC CCGAGGAGGA TCTGTTCGAG TTTGCCCTGG ACTATAAGAA
4081     CTTCTCTCGC ACAGACGCCG ATTACATCAA GAAGTGGAAG CTGTACTCCT ACGGCAACCG
4141     GATCAGAATC TTCCGGAATC CTAAGAAGAA CAACGTGTTC GACTGGGAGG AGGTGTGCCT
4201     GACCAGCGCC TATAAGGAGC TGTTCAACAA GTACGGCATC AATTATCAGC AGGGCGATAT
4261     CAGAGCCCTG CTGTGCGAGC AGTCCGACAA GGCCTTCTAC TCTAGCTTTA TGGCCCTGAT
4321     GAGCCTGATG CTGCAGATGC GGAACAGCAT CACAGGCCGC ACCGACGTGG ATTTTCTGAT
4381     CAGCCCTGTG AAGAACTCCG ACGGCATCTT CTACGATAGC CGGAACTATG AGGCCCAGGA
4441     GAATGCCATC CTGCCAAAGA ACGCCGACGC CAATGGCGCC TATAACATCG CCAGAAAGGT
4501     GCTGTGGGCC ATCGGCCAGT TCAAGAAGGC CGAGGACGAG AAGCTGGATA AGGTGAAGAT
4561     CGCCATCTCT AACAAGGAGT GGCTGGAGTA CGCCCAGACC AGCGTGAAGC ACAAAAGGCC
4621     GGCGGCCACG AAAAAGGCCG GCCAGGCAAA AAAGAAAAAG GGATCCTACC CATACGATGT
4681     TCCAGATTAC GCTTATCCCT ACGACGTGCC TGATTATGCA TACCCATATG ATGTCCCCGA
4741     CTATGCCTAA GAATTCTGCA GATATCCAGC ACAGTGGCGG CCGCTCGAGT CTAGAGGGCC
4801     CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG
4861     CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA
4921     AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT
4981     GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT
5041     GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC
5101     GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC
5161     ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT
5221     CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC
5281     TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC
5341     GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT
5401     CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG
5461     GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC
5521     GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA
5581     GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
5641     GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC
5701     CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC
5761     CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA
5821     TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA
5881     GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT
5941     GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT
6001     GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG
6061     GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC
6121     GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC
6181     GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC
6241     CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG
6301     CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG
6361     CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT
6421     CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG
6481     GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC
6541     TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG
6601     TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG
6661     CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG
6721     TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT
6781     CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC
6841     GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC
6901     CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA
6961     CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT
7021     CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC
7081     TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA
7141     TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT
7201     CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC
7261     GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
7321     TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT
7381     TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG
7441     CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG
7501     AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT
7561     ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
7621     CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT
7681     GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
7741     CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA
7801     GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG
7861     TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG
7921     TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT
7981     GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
8041     GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT
8101     GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT
8161     AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT
8221     GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC
8281     GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC
8341     CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT
8401     CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG
8461     CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA
8521     GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA
8581     TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT
8641     GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG
8701     TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA
8761     GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC
8821     GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT
8881     TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC
8941     TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA
9001     CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA
9061     TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA
9121     TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC
9181     AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTC
 


 
 上一篇:pY011 (pcDNA3.1-hLb2Cpf1) CRISPR/CAS9基因编辑载体 Cas12a
 下一篇:pY004 (pcDNA3.1-hFnCpf1) CRISPR/CAS9基因编辑载体 Cas12a

上海海吉浩格生物科技有限公司 版权所有   技术支持:创世网络  


地址:合肥市恒山路桃花智谷嘉创业园5号202-1


电话:17031145927

qq/微信:1822235623

 邮箱:wangdao@hedgehogbio.com


备案号:沪ICP备16027003号-1