测序及更多信息： https://pan.baidu.com/s/1FM6iku2JAhKW3QAmE9Hzmw?pwd=hedg
采购地址：淘宝店铺地址
载体基本信息
|
载体名称:
|
pY004 (pcDNA3.1-hFnCpf1)
|
|
质粒类型:
|
哺乳动物细胞表达载体CRISPR Cas12a
|
|
启动子:
|
CMV
|
|
表达水平:
|
高
|
|
克隆方法:
|
多克隆位点，限制性内切酶
|
|
载体大小:
|
9443 bp
|
|
5' 测序引物:
|
CMV-F: CGCAAATGGGCGGTAGGCGTG
|
|
3' 测序引物:
|
BGH-R:TAGAAGGCACAGTCGAGG
|
|
载体抗性:
|
氨苄
|
|
筛选标记:
|
Neo/G418
|
|
备注:
|
|
|
稳定性:
|
瞬表达
|
|
组成型:
|
组成型
|
|
病毒/非病毒:
|
非病毒
|
参考文献
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. 10.1016/j.cell.2015.09.038 PubMed 26422227
载体图谱↑蓝色为测序比对区
详细信息
全序列
1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG
61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG
121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC
181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT
241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA
301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC
361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC
421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT
481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT
541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA
601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG
661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC
721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG
781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA
841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC
901 GTTTAAACTT AAGCTTGGTA CCGCCACCAT GAGCATCTAC CAGGAGTTCG TCAACAAGTA
961 TTCACTGAGT AAGACACTGC GGTTCGAGCT GATCCCACAG GGCAAGACAC TGGAGAACAT
1021 CAAGGCCCGA GGCCTGATTC TGGACGATGA GAAGCGGGCA AAAGACTATA AGAAAGCCAA
1081 GCAGATCATT GATAAATACC ACCAGTTCTT TATCGAGGAA ATTCTGAGCT CCGTGTGCAT
1141 CAGTGAGGAT CTGCTGCAGA ATTACTCAGA CGTGTACTTC AAGCTGAAGA AGAGCGACGA
1201 TGACAACCTG CAGAAGGACT TCAAGTCCGC CAAGGACACC ATCAAGAAAC AGATTAGCGA
1261 GTACATCAAG GACTCCGAAA AGTTTAAAAA TCTGTTCAAC CAGAATCTGA TCGATGCTAA
1321 GAAAGGCCAG GAGTCCGACC TGATCCTGTG GCTGAAACAG TCTAAGGACA ATGGGATTGA
1381 ACTGTTCAAG GCTAACTCCG ATATCACTGA TATTGACGAG GCACTGGAAA TCATCAAGAG
1441 CTTCAAGGGA TGGACCACAT ACTTTAAAGG CTTCCACGAG AACCGCAAGA ACGTGTACTC
1501 CAGCAACGAC ATTCCTACCT CCATCATCTA CCGAATCGTC GATGACAATC TGCCAAAGTT
1561 CCTGGAGAAC AAGGCCAAAT ATGAATCTCT GAAGGACAAA GCTCCCGAGG CAATTAATTA
1621 CGAACAGATC AAGAAAGATC TGGCTGAGGA ACTGACATTC GATATCGACT ATAAGACTAG
1681 CGAGGTGAAC CAGAGGGTCT TTTCCCTGGA CGAGGTGTTT GAAATCGCCA ATTTCAACAA
1741 TTACCTGAAC CAGTCCGGCA TTACTAAATT CAATACCATC ATTGGCGGGA AGTTTGTGAA
1801 CGGGGAGAAT ACCAAGCGCA AGGGAATTAA CGAATACATC AATCTGTATA GCCAGCAGAT
1861 CAACGACAAA ACTCTGAAGA AATACAAGAT GTCTGTGCTG TTCAAACAGA TCCTGAGTGA
1921 TACCGAGTCC AAGTCTTTTG TCATTGATAA ACTGGAAGAT GACTCAGACG TGGTCACTAC
1981 CATGCAGAGC TTTTATGAGC AGATCGCCGC TTTCAAGACA GTGGAGGAAA AATCTATTAA
2041 GGAAACTCTG AGTCTGCTGT TCGATGACCT GAAAGCCCAG AAGCTGGACC TGAGTAAGAT
2101 CTACTTCAAA AACGATAAGA GTCTGACAGA CCTGTCACAG CAGGTGTTTG ATGACTATTC
2161 CGTGATTGGG ACCGCCGTCC TGGAGTACAT TACACAGCAG ATCGCTCCAA AGAACCTGGA
2221 TAATCCCTCT AAGAAAGAGC AGGAACTGAT CGCTAAGAAA ACCGAGAAGG CAAAATATCT
2281 GAGTCTGGAA ACAATTAAGC TGGCACTGGA GGAGTTCAAC AAGCACAGGG ATATTGACAA
2341 ACAGTGCCGC TTTGAGGAAA TCCTGGCCAA CTTCGCAGCC ATCCCCATGA TTTTTGATGA
2401 GATCGCCCAG AACAAAGACA ATCTGGCTCA GATCAGTATT AAGTACCAGA ACCAGGGCAA
2461 GAAAGACCTG CTGCAGGCTT CAGCAGAAGA TGACGTGAAA GCCATCAAGG ATCTGCTGGA
2521 CCAGACCAAC AATCTGCTGC ACAAGCTGAA AATCTTCCAT ATTAGTCAGT CAGAGGATAA
2581 GGCTAATATC CTGGATAAAG ACGAACACTT CTACCTGGTG TTCGAGGAAT GTTACTTCGA
2641 GCTGGCAAAC ATTGTCCCCC TGTATAACAA GATTAGGAAC TACATCACAC AGAAGCCTTA
2701 CTCTGACGAG AAGTTTAAAC TGAACTTCGA AAATAGTACC CTGGCCAACG GGTGGGATAA
2761 GAACAAGGAG CCTGACAACA CAGCTATCCT GTTCATCAAG GATGACAAGT ACTATCTGGG
2821 AGTGATGAAT AAGAAAAACA ATAAGATCTT CGATGACAAA GCCATTAAGG AGAACAAAGG
2881 GGAAGGATAC AAGAAAATCG TGTATAAGCT GCTGCCCGGC GCAAATAAGA TGCTGCCTAA
2941 GGTGTTCTTC AGCGCCAAGA GTATCAAATT CTACAACCCA TCCGAGGACA TCCTGCGGAT
3001 TAGAAATCAC TCAACACATA CTAAGAACGG GAGCCCCCAG AAGGGATATG AGAAATTTGA
3061 GTTCAACATC GAGGATTGCA GGAAGTTTAT TGACTTCTAC AAGCAGAGCA TCTCCAAACA
3121 CCCTGAATGG AAGGATTTTG GCTTCCGGTT TTCCGACACA CAGAGATATA ACTCTATCGA
3181 CGAGTTCTAC CGCGAGGTGG AAAATCAGGG GTATAAGCTG ACTTTTGAGA ACATTTCTGA
3241 AAGTTACATC GACAGCGTGG TCAATCAGGG AAAGCTGTAC CTGTTCCAGA TCTATAACAA
3301 AGATTTTTCA GCATACAGCA AGGGCAGACC AAACCTGCAT ACACTGTACT GGAAGGCCCT
3361 GTTCGATGAG AGGAATCTGC AGGACGTGGT CTATAAACTG AACGGAGAGG CCGAACTGTT
3421 TTACCGGAAG CAGTCTATTC CTAAGAAAAT CACTCACCCA GCTAAGGAGG CCATCGCTAA
3481 CAAGAACAAG GACAATCCTA AGAAAGAGAG CGTGTTCGAA TACGATCTGA TTAAGGACAA
3541 GCGGTTCACC GAAGATAAGT TCTTTTTCCA TTGTCCAATC ACCATTAACT TCAAGTCAAG
3601 CGGCGCTAAC AAGTTCAACG ACGAGATCAA TCTGCTGCTG AAGGAAAAAG CAAACGATGT
3661 GCACATCCTG AGCATTGACC GAGGAGAGCG GCATCTGGCC TACTATACCC TGGTGGATGG
3721 CAAAGGGAAT ATCATTAAGC AGGATACATT CAACATCATT GGCAATGACC GGATGAAAAC
3781 CAACTACCAC GATAAACTGG CTGCAATCGA GAAGGATAGA GACTCAGCTA GGAAGGACTG
3841 GAAGAAAATC AACAACATTA AGGAGATGAA GGAAGGCTAT CTGAGCCAGG TGGTCCATGA
3901 GATTGCAAAG CTGGTCATCG AATACAATGC CATTGTGGTG TTCGAGGATC TGAACTTCGG
3961 CTTTAAGAGG GGGCGCTTTA AGGTGGAAAA ACAGGTCTAT CAGAAGCTGG AGAAAATGCT
4021 GATCGAAAAG CTGAATTACC TGGTGTTTAA AGATAACGAG TTCGACAAGA CCGGAGGCGT
4081 CCTGAGAGCC TACCAGCTGA CAGCTCCCTT TGAAACTTTC AAGAAAATGG GAAAACAGAC
4141 AGGCATCATC TACTATGTGC CAGCCGGATT CACTTCCAAG ATCTGCCCCG TGACCGGCTT
4201 TGTCAACCAG CTGTACCCTA AATATGAGTC AGTGAGCAAG TCCCAGGAAT TTTTCAGCAA
4261 GTTCGATAAG ATCTGTTATA ATCTGGACAA GGGGTACTTC GAGTTTTCCT TCGATTACAA
4321 GAACTTCGGC GACAAGGCCG CTAAGGGGAA ATGGACCATT GCCTCCTTCG GATCTCGCCT
4381 GATCAACTTT CGAAATTCCG ATAAAAACCA CAATTGGGAC ACTAGGGAGG TGTACCCAAC
4441 CAAGGAGCTG GAAAAGCTGC TGAAAGACTA CTCTATCGAG TATGGACATG GCGAATGCAT
4501 CAAGGCAGCC ATCTGTGGCG AGAGTGATAA GAAATTTTTC GCCAAGCTGA CCTCAGTGCT
4561 GAATACAATC CTGCAGATGC GGAACTCAAA GACCGGGACA GAACTGGACT ATCTGATTAG
4621 CCCCGTGGCT GATGTCAACG GAAACTTCTT CGACAGCAGA CAGGCACCCA AAAATATGCC
4681 TCAGGATGCA GACGCCAACG GGGCCTACCA CATCGGGCTG AAGGGACTGA TGCTGCTGGG
4741 CCGGATCAAG AACAATCAGG AGGGGAAGAA GCTGAACCTG GTCATTAAGA ACGAGGAATA
4801 CTTCGAGTTT GTCCAGAATA GAAATAACAA AAGGCCGGCG GCCACGAAAA AGGCCGGCCA
4861 GGCAAAAAAG AAAAAGGGAT CCTACCCATA CGATGTTCCA GATTACGCTT ATCCCTACGA
4921 CGTGCCTGAT TATGCATACC CATATGATGT CCCCGACTAT GCCTAAGAAT TCTGCAGATA
4981 TCCAGCACAG TGGCGGCCGC TCGAGTCTAG AGGGCCCGTT TAAACCCGCT GATCAGCCTC
5041 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC
5101 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG
5161 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA
5221 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA
5281 AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC
5341 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC
5401 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT
5461 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA
5521 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC
5581 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT
5641 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG
5701 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT
5761 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT
5821 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG
5881 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG
5941 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT
6001 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT
6061 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
6121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
6181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
6241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
6301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
6361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
6421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
6481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
6541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
6601 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG
6661 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT
6721 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC
6781 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA
6841 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT
6901 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT
6961 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG
7021 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC
7081 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA
7141 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC
7201 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA
7261 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC
7321 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT
7381 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA
7441 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA
7501 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC
7561 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG
7621 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT
7681 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
7741 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT
7801 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
7861 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG
7921 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT
7981 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
8041 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA
8101 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA
8161 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG
8221 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
8281 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
8341 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
8401 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
8461 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC
8521 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
8581 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
8641 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
8701 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
8761 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC
8821 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG
8881 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
8941 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
9001 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
9061 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC
9121 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC
9181 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
9241 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
9301 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
9361 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC
9421 CCCGAAAAGT GCCACCTGAC GTC
