测序及更多信息： https://pan.baidu.com/s/1FM6iku2JAhKW3QAmE9Hzmw?pwd=hedg
采购地址：淘宝店铺地址
载体基本信息
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载体名称:
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pY011 (pcDNA3.1-hLb2Cpf1)
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质粒类型:
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哺乳动物细胞表达载体CRISPR Cas12a
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启动子:
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CMV
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表达水平:
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高
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克隆方法:
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多克隆位点，限制性内切酶
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载体大小:
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9164 bp
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5' 测序引物:
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CMV-F: CGCAAATGGGCGGTAGGCGTG
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3' 测序引物:
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BGH-R:TAGAAGGCACAGTCGAGG
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载体抗性:
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氨苄
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筛选标记:
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Neo/G418
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备注:
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稳定性:
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瞬表达
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组成型:
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组成型
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病毒/非病毒:
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非病毒
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全序列
1 AGATATACGC GTTGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA
61 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT
121 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA
181 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC
241 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT
301 AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG
361 TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT
421 GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT
481 GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC
541 CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCTC
601 TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC TCACTATAGG
661 GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGGTAC CGCCACCATG TACTATGAGT
721 CCCTGACCAA GCAGTACCCC GTGTCTAAGA CAATCCGGAA TGAGCTGATC CCTATCGGCA
781 AGACACTGGA TAACATCCGC CAGAACAATA TCCTGGAGAG CGACGTGAAG CGGAAGCAGA
841 ACTACGAGCA CGTGAAGGGC ATCCTGGATG AGTATCACAA GCAGCTGATC AACGAGGCCC
901 TGGACAATTG CACCCTGCCA TCCCTGAAGA TCGCCGCCGA GATCTACCTG AAGAATCAGA
961 AGGAGGTGTC TGACAGAGAG GATTTCAACA AGACACAGGA CCTGCTGAGG AAGGAGGTGG
1021 TGGAGAAGCT GAAGGCCCAC GAGAACTTTA CCAAGATCGG CAAGAAGGAC ATCCTGGATC
1081 TGCTGGAGAA GCTGCCTTCC ATCTCTGAGG ACGATTACAA TGCCCTGGAG AGCTTCCGCA
1141 ACTTTTACAC CTATTTCACA TCCTACAACA AGGTGCGGGA GAATCTGTAT TCTGATAAGG
1201 AGAAGAGCTC CACAGTGGCC TACAGACTGA TCAACGAGAA TTTCCCAAAG TTTCTGGACA
1261 ATGTGAAGAG CTATAGGTTT GTGAAAACCG CAGGCATCCT GGCAGATGGC CTGGGAGAGG
1321 AGGAGCAGGA CTCCCTGTTC ATCGTGGAGA CATTCAACAA GACCCTGACA CAGGACGGCA
1381 TCGATACCTA CAATTCTCAA GTGGGCAAGA TCAACTCTAG CATCAATCTG TATAACCAGA
1441 AGAATCAGAA GGCCAATGGC TTCAGAAAGA TCCCCAAGAT GAAGATGCTG TATAAGCAGA
1501 TCCTGTCCGA TAGGGAGGAG TCTTTCATCG ACGAGTTTCA GAGCGATGAG GTGCTGATCG
1561 ACAACGTGGA GTCTTATGGC AGCGTGCTGA TCGAGTCTCT GAAGTCCTCT AAGGTGAGCG
1621 CCTTCTTTGA TGCCCTGAGA GAGTCTAAGG GCAAGAACGT GTACGTGAAG AATGACCTGG
1681 CCAAGACAGC CATGAGCAAC ATCGTGTTCG AGAATTGGAG GACCTTTGAC GATCTGCTGA
1741 ACCAGGAGTA CGACCTGGCC AACGAGAACA AGAAGAAGGA CGATAAGTAT TTCGAGAAGC
1801 GCCAGAAGGA GCTGAAGAAG AATAAGAGCT ACTCCCTGGA GCACCTGTGC AACCTGTCCG
1861 AGGATTCTTG TAACCTGATC GAGAATTATA TCCACCAGAT CTCCGACGAT ATCGAGAATA
1921 TCATCATCAA CAATGAGACA TTCCTGCGCA TCGTGATCAA TGAGCACGAC AGGTCCCGCA
1981 AGCTGGCCAA GAACCGGAAG GCCGTGAAGG CCATCAAGGA CTTTCTGGAT TCTATCAAGG
2041 TGCTGGAGCG GGAGCTGAAG CTGATCAACA GCTCCGGCCA GGAGCTGGAG AAGGATCTGA
2101 TCGTGTACTC TGCCCACGAG GAGCTGCTGG TGGAGCTGAA GCAGGTGGAC AGCCTGTATA
2161 ACATGACCAG AAATTATCTG ACAAAGAAGC CTTTCTCTAC CGAGAAGGTG AAGCTGAACT
2221 TTAATCGCAG CACACTGCTG AACGGCTGGG ATCGGAATAA GGAGACAGAC AACCTGGGCG
2281 TGCTGCTGCT GAAGGACGGC AAGTACTATC TGGGCATCAT GAACACAAGC GCCAATAAGG
2341 CCTTCGTGAA TCCCCCTGTG GCCAAGACCG AGAAGGTGTT TAAGAAGGTG GATTACAAGC
2401 TGCTGCCAGT GCCCAACCAG ATGCTGCCAA AGGTGTTCTT TGCCAAGAGC AATATCGACT
2461 TCTATAACCC CTCTAGCGAG ATCTACTCCA ATTATAAGAA GGGCACCCAC AAGAAGGGCA
2521 ATATGTTTTC CCTGGAGGAT TGTCACAACC TGATCGACTT CTTTAAGGAG TCTATCAGCA
2581 AGCACGAGGA CTGGAGCAAG TTCGGCTTTA AGTTCAGCGA TACAGCCTCC TACAACGACA
2641 TCTCCGAGTT CTATCGCGAG GTGGAGAAGC AGGGCTACAA GCTGACCTAT ACAGACATCG
2701 ATGAGACATA CATCAATGAT CTGATCGAGC GGAACGAGCT GTACCTGTTC CAGATCTATA
2761 ATAAGGACTT TAGCATGTAC TCCAAGGGCA AGCTGAACCT GCACACACTG TATTTCATGA
2821 TGCTGTTTGA TCAGCGCAAT ATCGACGACG TGGTGTATAA GCTGAACGGA GAGGCAGAGG
2881 TGTTCTATAG GCCAGCCTCC ATCTCTGAGG ACGAGCTGAT CATCCACAAG GCCGGCGAGG
2941 AGATCAAGAA CAAGAATCCT AACCGGGCCA GAACCAAGGA GACAAGCACC TTCAGCTACG
3001 ACATCGTGAA GGATAAGCGG TATAGCAAGG ATAAGTTTAC CCTGCACATC CCCATCACAA
3061 TGAACTTCGG CGTGGATGAG GTGAAGCGGT TCAACGACGC CGTGAACAGC GCCATCCGGA
3121 TCGATGAGAA TGTGAACGTG ATCGGCATCG ACCGGGGCGA GAGAAATCTG CTGTACGTGG
3181 TGGTCATCGA CTCTAAGGGC AACATCCTGG AGCAGATCTC CCTGAACTCT ATCATCAATA
3241 AGGAGTACGA CATCGAGACA GATTATCACG CACTGCTGGA TGAGAGGGAG GGCGGCAGAG
3301 ATAAGGCCCG GAAGGACTGG AACACCGTGG AGAATATCAG GGACCTGAAG GCCGGCTACC
3361 TGAGCCAGGT GGTGAACGTG GTGGCCAAGC TGGTGCTGAA GTATAATGCC ATCATCTGCC
3421 TGGAGGACCT GAACTTTGGC TTCAAGAGGG GCCGCCAGAA GGTGGAGAAG CAGGTGTACC
3481 AGAAGTTCGA GAAGATGCTG ATCGATAAGC TGAATTACCT GGTCATCGAC AAGAGCCGCG
3541 AGCAGACATC CCCTAAGGAG CTGGGAGGCG CCCTGAACGC ACTGCAGCTG ACCTCTAAGT
3601 TCAAGAGCTT TAAGGAGCTG GGCAAGCAGT CCGGCGTGAT CTACTATGTG CCTGCCTACC
3661 TGACCTCTAA GATCGATCCA ACCACAGGCT TCGCCAATCT GTTTTATATG AAGTGTGAGA
3721 ACGTGGAGAA GTCCAAGAGA TTCTTTGACG GCTTTGATTT CATCAGGTTC AACGCCCTGG
3781 AGAACGTGTT CGAGTTCGGC TTTGACTACC GGAGCTTCAC CCAGAGGGCC TGCGGCATCA
3841 ATTCCAAGTG GACCGTGTGC ACCAACGGCG AGCGCATCAT CAAGTATCGG AATCCAGATA
3901 AGAACAATAT GTTCGACGAG AAGGTGGTGG TGGTGACCGA TGAGATGAAG AACCTGTTTG
3961 AGCAGTACAA GATCCCCTAT GAGGATGGCA GAAATGTGAA GGACATGATC ATCAGCAACG
4021 AGGAGGCCGA GTTCTACCGG AGACTGTATA GGCTGCTGCA GCAGACCCTG CAGATGAGAA
4081 ACAGCACCTC CGACGGCACA AGGGATTACA TCATCTCCCC TGTGAAGAAT AAGAGAGAGG
4141 CCTACTTCAA CAGCGAGCTG TCCGACGGCT CTGTGCCAAA GGACGCCGAT GCCAACGGCG
4201 CCTACAATAT CGCCAGAAAG GGCCTGTGGG TGCTGGAGCA GATCAGGCAG AAGAGCGAGG
4261 GCGAGAAGAT CAATCTGGCC ATGACCAACG CCGAGTGGCT GGAGTATGCC CAGACACACC
4321 TGCTGAAAAG GCCGGCGGCC ACGAAAAAGG CCGGCCAGGC AAAAAAGAAA AAGGGATCCT
4381 ACCCATACGA TGTTCCAGAT TACGCTTATC CCTACGACGT GCCTGATTAT GCATACCCAT
4441 ATGATGTCCC CGACTATGCC TAAGAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG
4501 AGTCTAGAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC
4561 CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG
4621 TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC
4681 TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG
4741 CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG
4801 GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA
4861 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT
4921 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT
4981 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC
5041 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT
5101 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT
5161 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC
5221 AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC
5281 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG
5341 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC
5401 AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC
5461 CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC
5521 TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
5581 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
5641 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
5701 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
5761 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
5821 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
5881 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
5941 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
6001 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
6061 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
6121 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
6181 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
6241 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
6301 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
6361 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
6421 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
6481 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
6541 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
6601 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
6661 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
6721 CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA
6781 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
6841 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
6901 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA
6961 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG
7021 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
7081 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
7141 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
7201 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
7261 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
7321 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
7381 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
7441 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
7501 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
7561 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
7621 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
7681 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
7741 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
7801 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
7861 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
7921 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
7981 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
8041 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
8101 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
8161 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
8221 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
8281 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
8341 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
8401 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
8461 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
8521 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
8581 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
8641 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
8701 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
8761 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
8821 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
8881 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
8941 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
9001 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
9061 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
9121 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCC
