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酵母相关 质粒

pYES2.0 酿酒酵母蛋白表达载体
发布时间:2018-05-28 12:06:36 | 浏览次数:


测序及更多信息:https://pan.baidu.com/s/1MiFi0wQfLQI88f-xFoX7nQ 密码: 2wg7

采购地址:淘宝店铺地址

出品公司:

Invitrogen

载体名称:

pYES2

质粒类型:

酿酒酵母蛋白表达载体

表达水平:

高拷贝

诱导方法:

半乳糖

启动子:

GAL1

克隆方法:

多克隆位点,限制性内切酶

载体大小:

5857 bp

5' 测序引物及序列:

T7: TAATACGACTCACTATAGGG

3' 测序引物及序列:

CYC1 Terminator: GTGACATAACTAATTACATGATG

载体标签:

/

载体抗性:

氨苄

筛选标记:

URA3

备注:

利用半乳糖诱导蛋白在酿酒酵母中表达。

稳定性:

稳定 Stable

组成型/诱导型:

诱导型

病毒/非病毒:

非病毒









全序列

1        ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
61       CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
121      ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
181      CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
241      TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
301      TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
361      GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
421      CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
481      GACTCACTAT AGGGAATATT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT AACGGCCGCC
541      AGTGTGCTGG AATTCTGCAG ATATCCATCA CACTGGCGGC CGCTCGAGCA TGCATCTAGA
601      GGGCCGCATC ATGTAATTAG TTATGTCACG CTTACATTCA CGCCCTCCCC CCACATCCGC
661      TCTAACCGAA AAGGAAGGAG TTAGACAACC TGAAGTCTAG GTCCCTATTT ATTTTTTTAT
721      AGTTATGTTA GTATTAAGAA CGTTATTTAT ATTTCAAATT TTTCTTTTTT TTCTGTACAG
781      ACGCGTGTAC GCATGTAACA TTATACTGAA AACCTTGCTT GAGAAGGTTT TGGGACGCTC
841      GAAGGCTTTA ATTTGCAAGC TGCGGCCCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA
901      GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
961      GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
1021     TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
1081     AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
1141     AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
1201     CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
1261     TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
1321     AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
1381     GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
1441     TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
1501     ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC
1561     TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
1621     CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
1681     AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
1741     AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
1801     TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
1861     AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
1921     ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGCGCTT ACCATCTGGC
1981     CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
2041     AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATT
2101     CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
2161     AACGTTGTTG GCATTGCTAC AGGCATCGTG GTGTCACTCT CGTCGTTTGG TATGGCTTCA
2221     TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
2281     GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
2341     CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
2401     TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
2461     TGCTCTTGCC CGGCGTCAAT ACGGGATAAT AGTGTATCAC ATAGCAGAAC TTTAAAAGTG
2521     CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
2581     TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
2641     AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
2701     ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT GGGTAATAAC TGATATAATT
2761     AAATTGAAGC TCTAATTTGT GAGTTTAGTA TACATGCATT TACTTATAAT ACAGTTTTTT
2821     AGTTTTGCTG GCCGCATCTT CTCAAATATG CTTCCCAGCC TGCTTTTCTG TAACGTTCAC
2881     CCTCTACCTT AGCATCCCTT CCCTTTGCAA ATAGTCCTCT TCCAACAATA ATAATGTCAG
2941     ATCCTGTAGA GACCACATCA TCCACGGTTC TATACTGTTG ACCCAATGCG TCTCCCTTGT
3001     CATCTAAACC CACACCGGGT GTCATAATCA ACCAATCGTA ACCTTCATCT CTTCCACCCA
3061     TGTCTCTTTG AGCAATAAAG CCGATAACAA AATCTTTGTC GCTCTTCGCA ATGTCAACAG
3121     TACCCTTAGT ATATTCTCCA GTAGATAGGG AGCCCTTGCA TGACAATTCT GCTAACATCA
3181     AAAGGCCTCT AGGTTCCTTT GTTACTTCTT CTGCCGCCTG CTTCAAACCG CTAACAATAC
3241     CTGGGCCCAC CACACCGTGT GCATTCGTAA TGTCTGCCCA TTCTGCTATT CTGTATACAC
3301     CCGCAGAGTA CTGCAATTTG ACTGTATTAC CAATGTCAGC AAATTTTCTG TCTTCGAAGA
3361     GTAAAAAATT GTACTTGGCG GATAATGCCT TTAGCGGCTT AACTGTGCCC TCCATGGAAA
3421     AATCAGTCAA GATATCCACA TGTGTTTTTA GTAAACAAAT TTTGGGACCT AATGCTTCAA
3481     CTAACTCCAG TAATTCCTTG GTGGTACGAA CATCCAATGA AGCACACAAG TTTGTTTGCT
3541     TTTCGTGCAT GATATTAAAT AGCTTGGCAG CAACAGGACT AGGATGAGTA GCAGCACGTT
3601     CCTTATATGT AGCTTTCGAC ATGATTTATC TTCGTTTCCT GCAGGTTTTT GTTCTGTGCA
3661     GTTGGGTTAA GAATACTGGG CAATTTCATG TTTCTTCAAC ACTACATATG CGTATATATA
3721     CCAATCTAAG TCTGTGCTCC TTCCTTCGTT CTTCCTTCTG TTCGGAGATT ACCGAATCAA
3781     AAAAATTTCA AAGAAACCGA AATCAAAAAA AAGAATAAAA AAAAAATGAT GAATTGAATT
3841     GAAAAGCTAG CTTATCGATG ATAAGCTGTC AAAGATGAGA ATTAATTCCA CGGACTATAG
3901     ACTATACTAG ATACTCCGTC TACTGTACGA TACACTTCCG CTCAGGTCCT TGTCCTTTAA
3961     CGAGGCCTTA CCACTCTTTT GTTACTCTAT TGATCCAGCT CAGCAAAGGC AGTGTGATCT
4021     AAGATTCTAT CTTCGCGATG TAGTAAAACT AGCTAGACCG AGAAAGAGAC TAGAAATGCA
4081     AAAGGCACTT CTACAATGGC TGCCATCATT ATTATCCGAT GTGACGCTGC AGCTTCTCAA
4141     TGATATTCGA ATACGCTTTG AGGAGATACA GCCTAATATC CGACAAACTG TTTTACAGAT
4201     TTACGATCGT ACTTGTTACC CATCATTGAA TTTTGAACAT CCGAACCTGG GAGTTTTCCC
4261     TGAAACAGAT AGTATATTTG AACCTGTATA ATAATATATA GTCTAGCGCT TTACGGAAGA
4321     CAATGTATGT ATTTCGGTTC CTGGAGAAAC TATTGCATCT ATTGCATAGG TAATCTTGCA
4381     CGTCGCATCC CCGGTTCATT TTCTGCGTTT CCATCTTGCA CTTCAATAGC ATATCTTTGT
4441     TAACGAAGCA TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT
4501     TCAAACAAAG AATCTGAGCT GCATTTTTAC AGAACAGAAA TGCAACGCGA AAGCGCTATT
4561     TTACCAACGA AGAATCTGTG CTTCATTTTT GTAAAACAAA AATGCAACGC GACGAGAGCG
4621     CTAATTTTTC AAACAAAGAA TCTGAGCTGC ATTTTTACAG AACAGAAATG CAACGCGAGA
4681     GCGCTATTTT ACCAACAAAG AATCTATACT TCTTTTTTGT TCTACAAAAA TGCATCCCGA
4741     GAGCGCTATT TTTCTAACAA AGCATCTTAG ATTACTTTTT TTCTCCTTTG TGCGCTCTAT
4801     AATGCAGTCT CTTGATAACT TTTTGCACTG TAGGTCCGTT AAGGTTAGAA GAAGGCTACT
4861     TTGGTGTCTA TTTTCTCTTC CATAAAAAAA GCCTGACTCC ACTTCCCGCG TTTACTGATT
4921     ACTAGCGAAG CTGCGGGTGC ATTTTTTCAA GATAAAGGCA TCCCCGATTA TATTCTATAC
4981     CGATGTGGAT TGCGCATACT TTGTGAACAG AAAGTGATAG CGTTGATGAT TCTTCATTGG
5041     TCAGAAAATT ATGAACGGTT TCTTCTATTT TGTCTCTATA TACTACGTAT AGGAAATGTT
5101     TACATTTTCG TATTGTTTTC GATTCACTCT ATGAATAGTT CTTACTACAA TTTTTTTGTC
5161     TAAAGAGTAA TACTAGAGAT AAACATAAAA AATGTAGAGG TCGAGTTTAG ATGCAAGTTC
5221     AAGGAGCGAA AGGTGGATGG GTAGGTTATA TAGGGATATA GCACAGAGAT ATATAGCAAA
5281     GAGATACTTT TGAGCAATGT TTGTGGAAGC GGTATTCGCA ATGGGAAGCT CCACCCCGGT
5341     TGATAATCAG AAAAGCCCCA AAAACAGGAA GATTGTATAA GCAAATATTT AAATTGTAAA
5401     CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACGA
5461     ATAGCCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG
5521     TGTTGTTCCA GTTTCCAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG
5581     GCGAAAAAGG GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT
5641     TTTGGGGTCG AGGTGCCGTA AAGCAGTAAA TCGGAAGGGT AAACGGATGC CCCCATTTAG
5701     AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC
5761     GGGGGCTAGG GCGGTGGGAA GTGTAGGGGT CACGCTGGGC GTAACCACCA CACCCGCCGC
5821     GCTTAATGGG GCGCTACAGG GCGCGTGGGG ATGATCCACT AGT




 

 
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