酵母相关质粒

pYES2.0 酿酒酵母蛋白表达载体

发布时间:2018-05-28 12:06:36 | 浏览次数：

测序及更多信息：https://pan.baidu.com/s/1MiFi0wQfLQI88f-xFoX7nQ 密码: 2wg7

采购地址：淘宝店铺地址

出品公司:	Invitrogen
载体名称:	pYES2
质粒类型:	酿酒酵母蛋白表达载体
表达水平:	高拷贝
诱导方法:	半乳糖
启动子:	GAL1
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5857 bp
5' 测序引物及序列:	T7: TAATACGACTCACTATAGGG
3' 测序引物及序列:	CYC1 Terminator: GTGACATAACTAATTACATGATG
载体标签:	/
载体抗性:	氨苄
筛选标记:	URA3
备注:	利用半乳糖诱导蛋白在酿酒酵母中表达。
稳定性:	稳定 Stable
组成型/诱导型:	诱导型
病毒/非病毒:	非病毒

全序列

1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
481 GACTCACTAT AGGGAATATT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT AACGGCCGCC
541 AGTGTGCTGG AATTCTGCAG ATATCCATCA CACTGGCGGC CGCTCGAGCA TGCATCTAGA
601 GGGCCGCATC ATGTAATTAG TTATGTCACG CTTACATTCA CGCCCTCCCC CCACATCCGC
661 TCTAACCGAA AAGGAAGGAG TTAGACAACC TGAAGTCTAG GTCCCTATTT ATTTTTTTAT
721 AGTTATGTTA GTATTAAGAA CGTTATTTAT ATTTCAAATT TTTCTTTTTT TTCTGTACAG
781 ACGCGTGTAC GCATGTAACA TTATACTGAA AACCTTGCTT GAGAAGGTTT TGGGACGCTC
841 GAAGGCTTTA ATTTGCAAGC TGCGGCCCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA
901 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
961 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
1021 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
1081 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
1141 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
1201 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
1261 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
1321 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
1381 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
1441 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
1501 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC
1561 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
1621 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
1681 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
1741 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
1801 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
1861 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
1921 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGCGCTT ACCATCTGGC
1981 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
2041 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATT
2101 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
2161 AACGTTGTTG GCATTGCTAC AGGCATCGTG GTGTCACTCT CGTCGTTTGG TATGGCTTCA
2221 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
2281 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
2341 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
2401 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
2461 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT AGTGTATCAC ATAGCAGAAC TTTAAAAGTG
2521 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
2581 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
2641 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
2701 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT GGGTAATAAC TGATATAATT
2761 AAATTGAAGC TCTAATTTGT GAGTTTAGTA TACATGCATT TACTTATAAT ACAGTTTTTT
2821 AGTTTTGCTG GCCGCATCTT CTCAAATATG CTTCCCAGCC TGCTTTTCTG TAACGTTCAC
2881 CCTCTACCTT AGCATCCCTT CCCTTTGCAA ATAGTCCTCT TCCAACAATA ATAATGTCAG
2941 ATCCTGTAGA GACCACATCA TCCACGGTTC TATACTGTTG ACCCAATGCG TCTCCCTTGT
3001 CATCTAAACC CACACCGGGT GTCATAATCA ACCAATCGTA ACCTTCATCT CTTCCACCCA
3061 TGTCTCTTTG AGCAATAAAG CCGATAACAA AATCTTTGTC GCTCTTCGCA ATGTCAACAG
3121 TACCCTTAGT ATATTCTCCA GTAGATAGGG AGCCCTTGCA TGACAATTCT GCTAACATCA
3181 AAAGGCCTCT AGGTTCCTTT GTTACTTCTT CTGCCGCCTG CTTCAAACCG CTAACAATAC
3241 CTGGGCCCAC CACACCGTGT GCATTCGTAA TGTCTGCCCA TTCTGCTATT CTGTATACAC
3301 CCGCAGAGTA CTGCAATTTG ACTGTATTAC CAATGTCAGC AAATTTTCTG TCTTCGAAGA
3361 GTAAAAAATT GTACTTGGCG GATAATGCCT TTAGCGGCTT AACTGTGCCC TCCATGGAAA
3421 AATCAGTCAA GATATCCACA TGTGTTTTTA GTAAACAAAT TTTGGGACCT AATGCTTCAA
3481 CTAACTCCAG TAATTCCTTG GTGGTACGAA CATCCAATGA AGCACACAAG TTTGTTTGCT
3541 TTTCGTGCAT GATATTAAAT AGCTTGGCAG CAACAGGACT AGGATGAGTA GCAGCACGTT
3601 CCTTATATGT AGCTTTCGAC ATGATTTATC TTCGTTTCCT GCAGGTTTTT GTTCTGTGCA
3661 GTTGGGTTAA GAATACTGGG CAATTTCATG TTTCTTCAAC ACTACATATG CGTATATATA
3721 CCAATCTAAG TCTGTGCTCC TTCCTTCGTT CTTCCTTCTG TTCGGAGATT ACCGAATCAA
3781 AAAAATTTCA AAGAAACCGA AATCAAAAAA AAGAATAAAA AAAAAATGAT GAATTGAATT
3841 GAAAAGCTAG CTTATCGATG ATAAGCTGTC AAAGATGAGA ATTAATTCCA CGGACTATAG
3901 ACTATACTAG ATACTCCGTC TACTGTACGA TACACTTCCG CTCAGGTCCT TGTCCTTTAA
3961 CGAGGCCTTA CCACTCTTTT GTTACTCTAT TGATCCAGCT CAGCAAAGGC AGTGTGATCT
4021 AAGATTCTAT CTTCGCGATG TAGTAAAACT AGCTAGACCG AGAAAGAGAC TAGAAATGCA
4081 AAAGGCACTT CTACAATGGC TGCCATCATT ATTATCCGAT GTGACGCTGC AGCTTCTCAA
4141 TGATATTCGA ATACGCTTTG AGGAGATACA GCCTAATATC CGACAAACTG TTTTACAGAT
4201 TTACGATCGT ACTTGTTACC CATCATTGAA TTTTGAACAT CCGAACCTGG GAGTTTTCCC
4261 TGAAACAGAT AGTATATTTG AACCTGTATA ATAATATATA GTCTAGCGCT TTACGGAAGA
4321 CAATGTATGT ATTTCGGTTC CTGGAGAAAC TATTGCATCT ATTGCATAGG TAATCTTGCA
4381 CGTCGCATCC CCGGTTCATT TTCTGCGTTT CCATCTTGCA CTTCAATAGC ATATCTTTGT
4441 TAACGAAGCA TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT
4501 TCAAACAAAG AATCTGAGCT GCATTTTTAC AGAACAGAAA TGCAACGCGA AAGCGCTATT
4561 TTACCAACGA AGAATCTGTG CTTCATTTTT GTAAAACAAA AATGCAACGC GACGAGAGCG
4621 CTAATTTTTC AAACAAAGAA TCTGAGCTGC ATTTTTACAG AACAGAAATG CAACGCGAGA
4681 GCGCTATTTT ACCAACAAAG AATCTATACT TCTTTTTTGT TCTACAAAAA TGCATCCCGA
4741 GAGCGCTATT TTTCTAACAA AGCATCTTAG ATTACTTTTT TTCTCCTTTG TGCGCTCTAT
4801 AATGCAGTCT CTTGATAACT TTTTGCACTG TAGGTCCGTT AAGGTTAGAA GAAGGCTACT
4861 TTGGTGTCTA TTTTCTCTTC CATAAAAAAA GCCTGACTCC ACTTCCCGCG TTTACTGATT
4921 ACTAGCGAAG CTGCGGGTGC ATTTTTTCAA GATAAAGGCA TCCCCGATTA TATTCTATAC
4981 CGATGTGGAT TGCGCATACT TTGTGAACAG AAAGTGATAG CGTTGATGAT TCTTCATTGG
5041 TCAGAAAATT ATGAACGGTT TCTTCTATTT TGTCTCTATA TACTACGTAT AGGAAATGTT
5101 TACATTTTCG TATTGTTTTC GATTCACTCT ATGAATAGTT CTTACTACAA TTTTTTTGTC
5161 TAAAGAGTAA TACTAGAGAT AAACATAAAA AATGTAGAGG TCGAGTTTAG ATGCAAGTTC
5221 AAGGAGCGAA AGGTGGATGG GTAGGTTATA TAGGGATATA GCACAGAGAT ATATAGCAAA
5281 GAGATACTTT TGAGCAATGT TTGTGGAAGC GGTATTCGCA ATGGGAAGCT CCACCCCGGT
5341 TGATAATCAG AAAAGCCCCA AAAACAGGAA GATTGTATAA GCAAATATTT AAATTGTAAA
5401 CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACGA
5461 ATAGCCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG
5521 TGTTGTTCCA GTTTCCAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG
5581 GCGAAAAAGG GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT
5641 TTTGGGGTCG AGGTGCCGTA AAGCAGTAAA TCGGAAGGGT AAACGGATGC CCCCATTTAG
5701 AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC
5761 GGGGGCTAGG GCGGTGGGAA GTGTAGGGGT CACGCTGGGC GTAACCACCA CACCCGCCGC
5821 GCTTAATGGG GCGCTACAGG GCGCGTGGGG ATGATCCACT AGT