测序及更多信息：https://pan.baidu.com/s/1_kG-Pa_q1DBZDmr7cQuMcw 
提取码：rjnj 

采购地址：淘宝店铺地址

我们的官网  http://hedgehogbio.comhttp://hedgehogbio.com

pABAI载体基本信息：

pAbAi is a yeast reporter vector that can be used in one-hybrid assays to identify and characterize DNA-binding proteins. The vector, which was specifically designed for use with the Matchmaker™ Gold Yeast One-Hybrid Library Screening System (Cat. No. 630491), contains a multiple cloning

site (MCS) upstream of the yeast iso-1-cytochrome C minimal promoter(not shown) and the AUR1-C gene, an antibiotic resistance gene that confers resistance to Aureobasidin A (AbA; Cat. No. 630466). A cis-acting element (i.e., a target sequence) can be inserted into the MCS and used as bait to screen GAL4 AD/cDNA fusion libraries (e.g. Mate & Plate™ Libraries) for proteins that interact with the target sequence. Positive protein-DNA (i.e.,one-hybrid) interactions drive the expression of AUR1-C. As a result, one-hybrid interactions can be detected by selecting for yeast

that are resistant to AbA.

pAbAi cannot be propagated episomally in yeast; it can only be stably maintained through integration into the host genome. Integration is accomplished via homologous recombination between the vector’s URA3 gene and the ura3-52 locus of the yeast strain provided in theMatchmaker Gold Yeast One-Hybrid System. URA3 is a nutritional marker that can also be used for selection of recombinant yeast (1, 2). The vector also contains a Col E1 origin of replication and an ampicillin resistance gene (Ampr) for propagation and selection in E.coli.

全序列

1        AAGCTTGAAT TCGAGCTCGG TACCCGGGGA TCTGTCGACC TCGAGGCATG TGCTCTGTAT
61       GTATATAAAA CTCTTGTTTT CTTCTTTTCT CTAAATATTC TTTCCTTATA CATTAGGTCC
121      TTTGTAGCAT AAATTACTAT ACTTCTATAG ACACGCAAAC ACAAATACAC ACACTAAATT
181      AATAATGGCA AACCCTTTTT CGAGATGGTT TCTATCAGAG AGACCTCCAA ACTGCCATGT
241      AGCCGATTTA GAAACAAGTT TAGATCCCCA TCAAACGTTG TTGAAGGTGC AAAAATACAA
301      ACCCGCTTTA AGCGACTGGG TGCATTACAT CTTCTTGGGA TCCATCATGC TGTTTGTGTT
361      CATTACTAAT CCCGCACCTT GGATCTTCAA GATCCTTTTT TATTGTTTCT TGGGCACTTT
421      ATTCATCATT CCAGCTACGT CACAGTTTTT CTTCAATGCC TTGCCCATCC TAACATGGGT
481      GGCGCTGTAT TTCACTTCAT CGTACTTTCC AGATGACCGC AGGCCTCCTA TTACTGTCAA
541      AGTGTTACCA GCGGTGGAAA CAATTTTATA CGGCGACAAT TTAAGTGATA TTCTTGCAAC
601      ATCGACGAAT TCCTTTTTGG ACATTTTAGC ATGGTTACCG TACGGACTAT TTCATTATGG
661      GGCCCCATTT GTCGTTGCTG CCATCTTATT CGTATTTGGT CCACCAACTG TTTTGCAAGG
721      TTATGCTTTT GCATTTGGTT ATATGAACCT GTTTGGTGTT ATCATGCAAA ATGTCTTTCC
781      AGCCGCTCCC CCATGGTATA AAATTCTCTA TGGATTGCAA TCAGCCAACT ATGATATGCA
841      TGGCTCGCCT GGTGGATTAG CTAGAATTGA TAAGCTACTC GGTATTAATA TGTATACTAC
901      ATGTTTTTCA AATTCCTCCG TCATTTTCGG TGCTTTTCCT TCACTGCATT CCGGGTGTGC
961      TACTATGGAA GCCCTGTTTT TCTGTTATTG TTTTCCAAAA TTGAAGCCCT TGTTTATTGC
1021     TTATGTTTGC TGGTTATGGT GGTCAACTAT GTATCTGACA CACCATTATT TTGTAGACCT
1081     TATGGCAGGT TCTGTGCTGT CATACGTTAT TTTCCAGTAC ACAAAGTACA CACATTTACC
1141     AATTGTAGAT ACATCTCTTT TTTGCAGATG GTCATACACT TCAATTGAGA AATACGATAT
1201     ATCAAAGAGT GATCCATTGG CTGCAGATTC AAACGATATC GAAAGTGTCC CTTTGTCCAA
1261     CTTGGAACTT GACTTTGATC TTAATATGAC TGATGAACCC AGTGTAAGCC CTTCGTTATT
1321     TGATGGATCT ACTTCTGTTT CTCGTTCGTC CGCCACGTCT ATAACGTCAC TAGGTGTAAA
1381     GAGGGCTTAA GGAAATCCAT TATGTACTAT TTAAAAAACA CAAACTTTTG GATGTTCGGT
1441     TTATTCTTTT TCTTTTACTT TTTTATCATG GGAGCCTACT TCCCGTTTTT CCCGATTTGG
1501     CTACATGACA TCAACCATAT CAGCAAAAGT GATACGGGTA TTATTTTTGC CGCTATTTCT
1561     CTGTTCTCGC TATTATTCCA ACCGCTGTTT GGTCTGCTTT CTGACAAACT CGGCCTCGAC
1621     TCTAGCGAAT TAATTCGTAA TCATGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT
1681     CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG
1741     AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT
1801     GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG
1861     GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC
1921     GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
1981     AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT
2041     GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
2101     GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
2161     CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
2221     GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
2281     TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
2341     CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
2401     CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
2461     GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
2521     AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
2581     CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
2641     TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
2701     TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
2761     TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
2821     CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
2881     CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
2941     ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
3001     GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
3061     CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
3121     TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
3181     ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
3241     TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
3301     ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
3361     CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
3421     AACACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
3481     TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
3541     CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
3601     AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
3661     ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
3721     CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC
3781     CCGAAAAGTG CCACCTGGGT AATAACTGAT ATAATTAAAT TGAAGCTCTA ATTTGTGAGT
3841     TTAGTATACA TGCATTTACT TATAATACAG TTTTTTAGTT TTGCTGGCCG CATCTTCTCA
3901     AATATGCTTC CCAGCCTGCT TTTCTGTAAC GTTCACCCTC TACCTTAGCA TCCCTTCCCT
3961     TTGCAAATAG TCCTCTTCCA ACAATAATAA TGTCAGATCC TGTAGAGACC ACATCATCCA
4021     CGGTTCTATA CTGTTGACCC AATGCGTCTC CCTTGTCATC TAAACCCACA CCGGGTGTCA
4081     TAATCAACCA ATCGTAACCT TCATCTCTTC CACCCATGTC TCTTTGAGCA ATAAAGCCGA
4141     TAACAAAATC TTTGTCGCTC TTCGCAATGT CAACAGTACC CTTAGTATAT TCTCCAGTAG
4201     ATAGGGAGCC CTTGCATGAC AATTCTGCTA ACATCAAAAG GCCTCTAGGT TCCTTTGTTA
4261     CTTCTTCTGC CGCCTGCTTC AAACCGCTAA CAATACCTGG GCCCACCACA CCGTGTGCAT
4321     TCGTAATGTC TGCCCATTCT GCTATTCTGT ATACACCCGC AGAGTACTGC AATTTGACTG
4381     TATTACCAAT GTCAGCAAAT TTTCTGTCTT CGAAGAGTAA AAAATTGTAC TTGGCGGATA
4441     ATGCCTTTAG CGGCTTAACT GTGCCCTCCA TGGAAAAATC AGTCAAGATA TCCACATGTG
4501     TTTTTAGTAA ACAAATTTTG GGACCTAATG CTTCAACTAA CTCCAGTAAT TCCTTGGTGG
4561     TACGAACATC CAATGAAGCA CACAAGTTTG TTTGCTTTTC GTGCATGATA TTAAATAGCT
4621     TGGCAGCAAC AGGACTAGGA TGAGTAGCAG CACGTTCCTT ATATGTAGCT TTCGACATGA
4681     TTTATCTTCG TTTCCTGCAG GTTTTTGTTC TGTGCAGTTG GGTTAAGAAT ACTGGGCAAT
4741     TTCATGTTTC TTCAACACTA CATATGCGTA TATATACCAA TCTAAGTCTG TGCTCCTTCC
4801     TTCGTTCTTC CTTCTGTTCG GAGATTACCG AATCAAAAAA ATTTCAAGGA AACCGAAATC
4861     AAAAAAAAGA ATAAAAAAAA AATGATGAAT TGAA