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酵母相关 质粒

pYES2/CT 酵母系列 酿酒酵母表达载体
发布时间:2018-05-28 12:06:41 | 浏览次数:


测序及更多信息:https://pan.baidu.com/s/1-DXDlAUYAPqTFxHVDR-4gA 密码:vmm9

采购地址:淘宝店铺地址

我们的官网  http://hedgehogbio.comhttp://hedgehogbio.com/

载体基本信息

载体名称:

pYES2-CT, pYES2/CT

质粒类型:

酿酒酵母表达载体

表达水平:

高拷贝

诱导方法:

半乳糖

启动子:

GAL1

克隆方法:

多克隆位点,限制性内切酶

载体大小:

5963 bp

5' 测序引物及序列:

GAL1-F: AATATACCTCTATACTTTAACGTC

3' 测序引物及序列:

CYC1-R: GCGTGAATGTAAGCGTGAC

载体标签:

C-His, C-V5

载体抗性:

氨苄青霉素

筛选标记:

URA3报告基因

克隆菌株:

TOP10, E.coli

表达菌株:

INVSc1, Saccharomyces cerevisiae

备注:

酿酒酵母表达载体pYES2/CT含有URA3报告基因;
GAL1
启动子驱动目的基因高水平表达;半乳糖诱导目基因表达,葡萄糖阻遏目的基因表达,二者起到分子开关作用。

稳定性:

稳表达

组成型/诱导型:

诱导型

病毒/非病毒:

非病毒



全序列

1        ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
61       CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
121      ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
181      CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
241      TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
301      TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
361      GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
421      CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
481      GACTCACTAT AGGGAATATT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT AACGGCCGCC
541      AGTGTGCTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC
601      TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
661      CATCACCATC ACCATTGAGT TTAAACCCGC TGATCCTAGA GGGCCGCATC ATGTAATTAG
721      TTATGTCACG CTTACATTCA CGCCCTCCCC CCACATCCGC TCTAACCGAA AAGGAAGGAG
781      TTAGACAACC TGAAGTCTAG GTCCCTATTT ATTTTTTTAT AGTTATGTTA GTATTAAGAA
841      CGTTATTTAT ATTTCAAATT TTTCTTTTTT TTCTGTACAG ACGCGTGTAC GCATGTAACA
901      TTATACTGAA AACCTTGCTT GAGAAGGTTT TGGGACGCTC GAAGGCTTTA ATTTGCAAGC
961      TGCGGCCCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
1021     CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
1081     ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
1141     GAACATGTGA GCAAAAGGCC AGCAAAAGCC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
1201     GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
1261     GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
1321     GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
1381     AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
1441     CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
1501     TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
1561     TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
1621     GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
1681     TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
1741     TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
1801     TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
1861     GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
1921     TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
1981     TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
2041     CGTGTAGATA ACTACGATAC GGGAGCGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
2101     GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
2161     CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
2221     GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG GCATTGCTAC
2281     AGGCATCGTG GTGTCACTCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
2341     ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
2401     TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
2461     GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
2521     AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT
2581     ACGGGATAAT AGTGTATCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
2641     TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
2701     TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
2761     AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
2821     CATACTCTTC CTTTTTCAAT GGGTAATAAC TGATATAATT AAATTGAAGC TCTAATTTGT
2881     GAGTTTAGTA TACATGCATT TACTTATAAT ACAGTTTTTT AGTTTTGCTG GCCGCATCTT
2941     CTCAAATATG CTTCCCAGCC TGCTTTTCTG TAACGTTCAC CCTCTACCTT AGCATCCCTT
3001     CCCTTTGCAA ATAGTCCTCT TCCAACAATA ATAATGTCAG ATCCTGTAGA GACCACATCA
3061     TCCACGGTTC TATACTGTTG ACCCAATGCG TCTCCCTTGT CATCTAAACC CACACCGGGT
3121     GTCATAATCA ACCAATCGTA ACCTTCATCT CTTCCACCCA TGTCTCTTTG AGCAATAAAG
3181     CCGATAACAA AATCTTTGTC GCTCTTCGCA ATGTCAACAG TACCCTTAGT ATATTCTCCA
3241     GTAGATAGGG AGCCCTTGCA TGACAATTCT GCTAACATCA AAAGGCCTCT AGGTTCCTTT
3301     GTTACTTCTT CTGCCGCCTG CTTCAAACCG CTAACAATAC CTGGGCCCAC CACACCGTGT
3361     GCATTCGTAA TGTCTGCCCA TTCTGCTATT CTGTATACAC CCGCAGAGTA CTGCAATTTG
3421     ACTGTATTAC CAATGTCAGC AAATTTTCTG TCTTCGAAGA GTAAAAAATT GTACTTGGCG
3481     GATAATGCCT TTAGCGGCTT AACTGTGCCC TCCATGGAAA AATCAGTCAA GATATCCACA
3541     TGTGTTTTTA GTAAACAAAT TTTGGGACCT AATGCTTCAA CTAACTCCAG TAATTCCTTG
3601     GTGGTACGAA CATCCAATGA AGCACACAAG TTTGTTTGCT TTTCGTGCAT GATATTAAAT
3661     AGCTTGGCAG CAACAGGACT AGGATGAGTA GCAGCACGTT CCTTATATGT AGCTTTCGAC
3721     ATGATTTATC TTCGTTTCCT GCAGGTTTTT GTTCTGTGCA GTTGGGTTAA GAATACTGGG
3781     CAATTTCATG TTTCTTCAAC ACTACATATG CGTATATATA CCAATCTAAG TCTGTGCTCC
3841     TTCCTTCGTT CTTCCTTCTG TTCGGAGATT ACCGAATCAA AAAAATTTCA AAGAAACCGA
3901     AATCAAAAAA AAGAATAAAA AAAAAATGAT GAATTGAATT GAAAAGCTAG CTTATCGATG
3961     ATAAGCTGTC AAAGATGAGA ATTAATTCCA CGGACTATAG ACTATACTAG ATACTCCGTC
4021     TACTGTACGA TACACTTCCG CTCAGGTCCT TGTCCTTTAA CGAGGCCTTA CCACTCTTTT
4081     GTTACTCTAT TGATCCAGCT CAGCAAAGGC AGTGTGATCT AAGATTCTAT CTTCGCGATG
4141     TAGTAAAACT AGCTAGACCG AGAAAGAGAC TAGAAATGCA AAAGGCACTT CTACAATGGC
4201     TGCCATCATT ATTATCCGAT GTGACGCTGC AGCTTCTCAA TGATATTCGA ATACGCTTTG
4261     AGGAGATACA GCCTAATATC CGACAAACTG TTTTACAGAT TTACGATCGT ACTTGTTACC
4321     CATCATTGAA TTTTGAACAT CCGAACCTGG GAGTTTTCCC TGAAACAGAT AGTATATTTG
4381     AACCTGTATA ATAATATATA GTCTAGCGCT TTACGGAAGA CAATGTATGT ATTTCGGTTC
4441     CTGGAGAAAC TATTGCATCT ATTGCATAGG TAATCTTGCA CGTCGCATCC CCGGTTCATT
4501     TTCTGCGTTT CCATCTTGCA CTTCAATAGC ATATCTTTGT TAACGAAGCA TCTGTGCTTC
4561     ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT TCAAACAAAG AATCTGAGCT
4621     GCATTTTTAC AGAACAGAAA TGCAACGCGA AAGCGCTATT TTACCAACGA AGAATCTGTG
4681     CTTCATTTTT GTAAAACAAA AATGCAACGC GACGAGAGCG CTAATTTTTC AAACAAAGAA
4741     TCTGAGCTGC ATTTTTACAG AACAGAAATG CAACGCGAGA GCGCTATTTT ACCAACAAAG
4801     AATCTATACT TCTTTTTTGT TCTACAAAAA TGCATCCCGA GAGCGCTATT TTTCTAACAA
4861     AGCATCTTAG ATTACTTTTT TTCTCCTTTG TGCGCTCTAT AATGCAGTCT CTTGATAACT
4921     TTTTGCACTG TAGGTCCGTT AAGGTTAGAA GAAGGCTACT TTGGTGTCTA TTTTCTCTTC
4981     CATAAAAAAA GCCTGACTCC ACTTCCCGCG TTTACTGATT ACTAGCGAAG CTGCGGGTGC
5041     ATTTTTTCAA GATAAAGGCA TCCCCGATTA TATTCTATAC CGATGTGGAT TGCGCATACT
5101     TTGTGAACAG AAAGTGATAG CGTTGATGAT TCTTCATTGG TCAGAAAATT ATGAACGGTT
5161     TCTTCTATTT TGTCTCTATA TACTACGTAT AGGAAATGTT TACATTTTCG TATTGTTTTC
5221     GATTCACTCT ATGAATAGTT CTTACTACAA TTTTTTTGTC TAAAGAGTAA TACTAGAGAT
5281     AAACATAAAA AATGTAGAGG TCGAGTTTAG ATGCAAGTTC AAGGAGCGAA AGGTGGATGG
5341     GTAGGTTATA TAGGGATATA GCACAGAGAT ATATAGCAAA GAGATACTTT TGAGCAATGT
5401     TTGTGGAAGC GGTATTCGCA ATGGGAAGCT CCACCCCGGT TGATAATCAG AAAAGCCCCA
5461     AAAACAGGAA GATTGTATAA GCAAATATTT AAATTGTAAA CGTTAATATT TTGTTAAAAT
5521     TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACGA ATAGCCCGAA ATCGGCAAAA
5581     TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTCCAACA
5641     AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAAGG GTCTATCAGG
5701     GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA
5761     AAGCAGTAAA TCGGAAGGGT AAACGGATGC CCCCATTTAG AGCTTGACGG GGAAAGCCGG
5821     CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGGGCTAGG GCGGTGGGAA
5881     GTGTAGGGGT CACGCTGGGC GTAACCACCA CACCCGCCGC GCTTAATGGG GCGCTACAGG
5941     GCGCGTGGGG ATGATCCACT AGT




 
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