测序及更多信息：https://pan.baidu.com/s/11A9pa_kKsMu_AcsxZRmZXw
提取码：w3yc
采购地址：淘宝店铺地址
pColdTF载体基本信息
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出品公司:
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Takara
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载体名称:
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pCold
TF, pColdTF, pCold-TF
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质粒类型:
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大肠杆菌蛋白表达载体
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表达水平:
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高
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启动子:
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CSPA
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克隆方法:
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多克隆位点，限制性内切酶
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载体大小:
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5769
bp
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5' 测序引物及序列:
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pCold-TF-F1:
5'-CCACTTTCAACGAGCTGATG-3';
pCold-TF-F2: 5'-GCGAAAGTGACTGAAAAAG-3'
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3' 测序引物及序列:
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pCold-R:
5'-GGCAGGGATCTTAGATTCTG-3'
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载体标签:
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N-His,
N-Trigger factor, N-HRV 3C, N-Thrombin, N-Factor Xa
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载体抗性:
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Ampicillin 氨苄
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备注:
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温度诱导，冷休克蛋白表达载体
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稳定性:
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瞬时表达 Transient
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组成型:
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诱导表达
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病毒/非病毒:
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非病毒
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全序列
1 AAGGAATGGT GTGGCCGATT AATCATAAAT ATGAAAAATA ATTGTTGCAT CACCCGCCAA
61 TGCGTGGCTT AATGCACATC AAATTGTGAG CGGATAACAA TTTGATGTGC TAGCGCATAT
121 CCAGTGTAGT AAGGCAAGTC CCTTCAAGAG TTATCGTTGA TACCCCTCGT AGTGCACATT
181 CCTTTAACGC TTCAAAATCT GTAAAGCACG CCATATCGCC GAAAGGCACA CTTAATTATT
241 AAGAGGTAAT ACACCATGAA TCACAAAGTG CATCATCATC ATCATCACAT GCAAGTTTCA
301 GTTGAAACCA CTCAAGGCCT TGGCCGCCGT GTAACGATTA CTATCGCTGC TGACAGCATC
361 GAGACCGCTG TTAAAAGCGA GCTGGTCAAC GTTGCGAAAA AAGTACGTAT TGACGGCTTC
421 CGCAAGGGCA AAGTGCCAAT GAATATCGTT GCTCAGCGTT ATGGCGCGTC TGTACGCCAG
481 GACGTTCTGG GTGACCTGAT GAGCCGTAAC TTCATTGACG CCATCATTAA AGAAAAAATC
541 AATCCGGCTG GCGCACCGAC TTATGTTCCG GGCGAATACA AGCTGGGTGA AGACTTCACT
601 TACTCTGTAG AGTTTGAAGT TTATCCGGAA GTTGAACTGC AAGGTCTGGA AGCGATCGAA
661 GTTGAAAAAC CGATCGTTGA AGTGACCGAC GCTGACGTTG ACGGCATGCT GGATACTCTG
721 CGTAAACAGC AGGCGACCTG GAAAGAAAAA GACGGCGCTG TTGAAGCAGA AGACCGCGTG
781 ACCATCGACT TCACCGGTTC TGTAGACGGC GAAGAGTTCG AAGGCGGTAA AGCGTCTGAT
841 TTCGTACTGG CGATGGGCCA GGGTCGTATG ATCCCGGGCT TTGAAGACGG TATCAAAGGC
901 CACAAAGCTG GCGAAGAGTT CACCATCGAC GTGACCTTCC CGGAAGAATA CCACGCAGAA
961 AACCTGAAAG GTAAAGCAGC GAAATTCGCT ATCAACCTGA AGAAAGTTGA AGAGCGTGAA
1021 CTGCCGGAAC TGACCGCAGA GTTCATCAAA CGTTTCGGCG TTGAAGATGG TTCCGTAGAA
1081 GGTCTGCGCG CTGAAGTGCG TAAAAACATG GAGCGCGAGC TGAAGAGCGC CATCCGTAAC
1141 CGCGTTAAGT CTCAGGCGAT CGAAGGTCTG GTAAAAGCTA ACGACATCGA CGTACCGGCT
1201 GCGCTGATCG ACAGCGAAAT CGACGTTCTG CGTCGCCAGG CTGCACAGCG TTTCGGTGGC
1261 AACGAAAAAC AAGCTCTGGA ACTGCCGCGC GAACTGTTCG AAGAACAGGC TAAACGCCGC
1321 GTAGTTGTTG GCCTGCTGCT GGGCGAAGTT ATCCGCACCA ACGAGCTGAA AGCTGACGAA
1381 GAGCGCGTGA AAGGCCTGAT CGAAGAGATG GCTTCTGCGT ACGAAGATCC GAAAGAAGTT
1441 ATCGAGTTCT ACAGCAAAAA CAAAGAACTG ATGGACAACA TGCGCAATGT TGCTCTGGAA
1501 GAACAGGCTG TTGAAGCTGT ACTGGCGAAA GCGAAAGTGA CTGAAAAAGA AACCACTTTC
1561 AACGAGCTGA TGAACCAGCA GGCGTCCGCG GGTCTGGAAG TTCTGTTCCA GGGGCCCTCC
1621 GCGGGTCTGG TGCCACGCGG TAGTGGTGGT ATCGAAGGTA GGCATATGGA GCTCGGTACC
1681 CTCGAGGGAT CCGAATTCAA GCTTGTCGAC CTGCAGTCTA GATAGGTAAT CTCTGCTTAA
1741 AAGCACAGAA TCTAAGATCC CTGCCATTTG GCGGGGATTT TTTTATTTGT TTTCAGGAAA
1801 TAAATAATCG ATCGCGTAAT AAAATCTATT ATTATTTTTG TGAAGAATAA ATTTGGGTGC
1861 AATGAGAATG CGCAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG
1921 ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA
1981 AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC
2041 ATCAGAGCAG ATTGTACTGA GAGTGCACCA TAAAATTGTA AACGTTAATA TTTTGTTAAA
2101 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA
2161 AATCCCTTAT AAATCAAAAG AATAGCCCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA
2221 CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA
2281 GGGCGATGGC CCACTACGTG AACCATCACC CAAATCAAGT TTTTTGGGGT CGAGGTGCCG
2341 TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC
2401 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC
2461 AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA
2521 GGGCGCGTAC TATGGTTGCT TTGACGTATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG
2581 AGAAAATACC GCATCAGGCG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA
2641 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT
2701 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC
2761 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA
2821 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA
2881 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT
2941 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG
3001 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC
3061 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA
3121 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT
3181 TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG
3241 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA
3301 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG
3361 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG
3421 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG
3481 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG
3541 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG
3601 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA
3661 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT
3721 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC
3781 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
3841 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
3901 CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
3961 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
4021 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
4081 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
4141 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
4201 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
4261 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
4321 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
4381 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC
4441 TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGAGATCC CGGTGCCTAA TGAGTGAGCT
4501 AACTTACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC
4561 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCCAGG
4621 GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC CGCCTGGCCC
4681 TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG
4741 ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG TATCGTCGTA TCCCACTACC
4801 GAGATATCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC GCCCAGCGCC
4861 ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG CATTTGCATG
4921 GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC GTTCCGCTAT CGGCTGAATT
4981 TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA GACAGAACTT
5041 AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG CGACCAGATG CTCCACGCCC
5101 AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG GTCAGAGACA
5161 TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC ATCCTGGTCA
5221 TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG CGAGAAGATT GTGCACCGCC
5281 GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT GGCACCCAGT
5341 TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG GCGCGTGCAG GGCCAGACTG
5401 GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC CACGCGGTTG
5461 GGAATGTAAT TCAGCTCCGC CATCGCCGCT TCCACTTTTT CCCGCGTTTT CGCAGAAACG
5521 TGGCTGGCCT GGTTCACCAC GCGGGAAACG GTCTGATAAG AGACACCGGC ATACTCTGCG
5581 ACATCGTATA ACGTTACTGG TTTCACATTC ACCACCCTGA ATTGACTCTC TTCCGGGCGC
5641 TATCATGCCA TACCGCGAAA GGTTTTGCGC CATTCGATGG TGTCCGGGAT CTCGACGCTC
5701 TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC
5761 CGCCGCCGC
