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载体名称:
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pColdS-SUMO
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质粒类型:
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大肠杆菌表达载体
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高拷贝/低拷贝:
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高
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启动子:
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CSPA
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克隆方法:
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多克隆位点，限制性内切酶
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载体大小:
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4740 bp
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5' 测序引物及序列:
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--
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3' 测序引物及序列:
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pCold-R: 5'-GGCAGGGATCTTAGATTCTG-3'
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载体标签:
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N-His, N-Factor Xa, N-SUMO
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载体抗性:
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Ampicillin
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备注:
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温度诱导，冷休克蛋白表达载体
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稳定性:
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瞬时表达
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组成型:
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诱导表达
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病毒/非病毒:
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非病毒
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1 AAGGAATGGT GTGGCCGATT AATCATAAAT ATGAAAAATA ATTGTTGCAT CACCCGCCAA
61 TGCGTGGCTT AATGCACATC AAATTGTGAG CGGATAACAA TTTGATGTGC TAGCGCATAT
121 CCAGTGTAGT AAGGCAAGTC CCTTCAAGAG TTATCGTTGA TACCCCTCGT AGTGCACATT
181 CCTTTAACGC TTCAAAATCT GTAAAGCACG CCATATCGCC GAAAGGCACA CTTAATTATT
241 AAGAGGTAAT ACACCATGAA TCACAAAGTG CATCATCATC ATCATCATAT CGAAGGTAGG
301 CATATGGGCG GTGGCGGTAG CGGCGGTGGC GGTAGCGGTA TGTCGGACTC AGAAGTCAAT
361 CAAGAAGCTA AGCCAGAGGT CAAGCCAGAA GTCAAGCCTG AGACTCACAT CAATTTAAAG
421 GTGTCCGATG GATCTTCAGA GATCTTCTTC AAGATCAAAA AGACCACTCC TTTAAGAAGG
481 CTGATGGAAG CGTTCGCTAA AAGACAGGGT AAGGAAATGG ACTCCTTAAG ATTCTTGTAC
541 GACGGTATTA GAATTCAAGC TGATCAGACC CCTGAAGATT TGGACATGGA GGATAACGAT
601 ATTATTGAGG CTCACAGAGA ACAGATTGGT GGTACTAGTG AGCTCGGTAC CCTCGAGGGA
661 TCCGAATTCA AGCTTGTCGA CCTGCAGTCT AGATAGGTAA TCTCTGCTTA AAAGCACAGA
721 ATCTAAGATC CCTGCCATTT GGCGGGGATT TTTTTATTTG TTTTCAGGAA ATAAATAATC
781 GATCGCGTAA TAAAATCTAT TATTATTTTT GTGAAGAATA AATTTGGGTG CAATGAGAAT
841 GCGCAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA
901 GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA
961 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA
1021 GATTGTACTG AGAGTGCACC ATAAAATTGT AAACGTTAAT ATTTTGTTAA AATTCGCGTT
1081 AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA
1141 TAAATCAAAA GAATAGCCCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC
1201 ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG
1261 CCCACTACGT GAACCATCAC CCAAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT
1321 AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT
1381 GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC
1441 GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTA
1501 CTATGGTTGC TTTGACGTAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC
1561 CGCATCAGGC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT
1621 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC
1681 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT
1741 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG
1801 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA
1861 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC
1921 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA
1981 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG
2041 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG
2101 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA
2161 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA
2221 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA
2281 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA
2341 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT
2401 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC
2461 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA
2521 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT
2581 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA
2641 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG
2701 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA
2761 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG
2821 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG
2881 TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT
2941 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA
3001 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG
3061 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC
3121 GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA
3181 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC
3241 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT
3301 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
3361 TTTGCTGGCC TTTTGCTCAC ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT
3421 TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT
3481 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT
3541 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT
3601 CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT
3661 TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT
3721 AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC
3781 GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG
3841 TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA
3901 AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA
3961 GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC
4021 GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA
4081 CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT
4141 AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA
4201 TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG
4261 GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG
4321 CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA
4381 ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA
4441 TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC
4501 TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT
4561 AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC
4621 ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG
4681 CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC
