测序及更多信息： https://pan.baidu.com/s/1NjYSYpFh3HPYNrqAJFoPqA
提取码：z9ft
采购地址：淘宝店铺地址
公司官网 http://hedgehogbio.com http://hedgehogbio.com
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载体名称:
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pDG1730
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质粒类型:
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枯草芽孢杆菌表达载体
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高拷贝/低拷贝:
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高
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启动子:
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--
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克隆方法:
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多克隆位点，限制性内切酶
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载体大小:
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7600
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5' 测序引物及序列:
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--
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3' 测序引物及序列:
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--
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载体标签:
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--
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载体抗性:
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Amp
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筛选标记:
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--
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稳定性:
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--
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组成型:
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--
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病毒/非病毒:
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非病毒
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全序列
1 AACAAAATTC TCCAGTCTTC ACATCGGTTT GAAAGGAGGA AGCGGAAGAA TGAAGTAAGA
61 GGGATTTTTG ACTCCGAAGT AAGTCTTCAA AAAATCAAAT AAGGAGTGTC AAGAATGTTT
121 GCAAAACGAT TCAAAACCTC TTTACTGCCG TTATTCGCTG GATTTTTATT GCTGTTTCAT
181 TTGGTTCTGG CAGGACCGGC GGCTGCGAGT GCTGAAACGG CGAACAAATC GAATGAGCTT
241 ACAGCACCGT CGATCAAAAG CGGAACCATT CTTCATGCAT GGAATTGGTC GTTCAATACG
301 TTAAAACACA ATATGAAGGA TATTCATGAT GCAGGATATA CAGCCATTCA GACATCTCCG
361 ATTAACCAAG TAAAGGAAGG GAATCAAGGA GATAAAAGCA TGTCGAACTG GTACTGGCTG
421 TATCAGCCGA CATCGTATCA AATTGGCAAC CGTTACTTAG GTACTGAACA AGAATTTAAA
481 GAAATGTGTG CAGCCGCTGA AGAATATGGC ATAAAGGTCA TTGTTGACGC GGTCATCAAT
541 CATACCACCA GTGATTATGC CGCGATTTCC AATGAGGTTA AGAGTATTCC AAACTGGACA
601 CATGGAAACA CACAAATTAA AAACTGGTCT GATCGGATCC TAGAAGCTTA TCGAATTCCT
661 GCAGCTGGCG AATGGCGATT TTCGTTCGTG AATACATGTT ATAATAACTA TAACTAATAA
721 CGTAACGTGA CTGGCAAGAG ATATTTTTAA AACAATGAAT AGGTTTACAC TTACTTTAGT
781 TTTATGGAAA TGAAAGATCA TATCATATAT AATCTAGAAT AAAATTAACT AAAATAATTA
841 TTATCTAGAT AAAAAATTTA GAAGCCAATG AAATCTATAA ATAAACTAAA TTAAGTTTAT
901 TTAATTAACA ACTATGGATA TAAAATAGGT ACTAATCAAA ATAGTGAGGA GGATATATTT
961 GAATACATAC GAACAAATTA ATAAAGTGAA AAAAATACTT CGGAAACATT TAAAAAATAA
1021 CCTTATTGGT ACTTACATGT TTGGATCAGG AGTTGAGAGT GGACTAAAAC CAAATAGTGA
1081 TCTTGACTTT TTAGTCGTCG TATCTGAACC ATTGACAGAT CAAAGTAAAG AAATACTTAT
1141 ACAAAAAATT AGACCTATTT CAAAAAAAAT AGGAGATAAA AGCAACTTAC GATATATTGA
1201 ATTAACAATT ATTATTCAGC AAGAAATGGT ACCGTGGAAT CATCCTCCCA AACAAGAATT
1261 TATTTATGGA GAATGGTTAC AAGAGCTTTA TGAACAAGGA TACATTCCTC AGAAGGAATT
1321 AAATTCAGAT TTAACCATAA TGCTTTACCA AGCAAAACGA AAAAATAAAA GAATATACGG
1381 AAATTATGAC TTAGAGGAAT TACTACCTGA TATTCCATTT TCTGATGTGA GAAGAGCCAT
1441 TATGGATTCG TCAGAGGAAT TAATAGATAA TTATCAGGAT GATGAAACCA ACTCTATATT
1501 AACTTTATGC CGTATGATTT TAACTATGGA CACGGGTAAA ATCATACCAA AAGATATTGC
1561 GGGAAATGCA GTGGCTGAAT CTTCTCCATT AGAACATAGG GAGAGAATTT TGTTAGCAGT
1621 TCGTAGTTAT CTTGGAGAGA ATATTGAATG GACTAATGAA AATGTAAATT TAACTATAAA
1681 CTATTTAAAT AACAGATTAA AAAAATTATA AAAAAATTGA AAAAATGGTG GAAACACTTT
1741 TTTCAATTTT TTTGTTTTAT TATTTAATAT TTGGGAAATA TTCATTCTAA TTGGTAATCA
1801 GATTTTAGAA AACAATAAAC CCTTGCATAG GGGGATCTCG ACATGGATGA GCGATGATGA
1861 TATCCGTTTA GGCTGGGCGG TGATAGCTTC TCGTTCAGGC AGTACGCCTC TTTTCTTTTC
1921 CAGACCTGAG GGAGGCGGAA ATGGTGTGAG GTTCCCGGGG AAAAGCCAAA TAGGCGATCG
1981 CGGGAGTGCT TTATTTGAAG ATCAGGCTAT CACTGCGGTC AATAGATTTC ACAATGTGAT
2041 GGCTGGACAG CCTGAGGAAC TCTCGAACCC GAATGGAAAC AACCAGATAT TTATGAATCA
2101 GCGCGGCTCA CATGGCGTTG TGCTGGCAAA TGCAGGTTCA TCCTCTGTCT CTATCAATAC
2161 GGCAACAAAA TTGCCTGATG GCAGGTATGA CAATAAAGCT GGAGCGGGTT CATTTCAAGT
2221 GAACGATGGT AAACTGACAG GCACGATCAA TGCCAGGTCT GTAGCTGTGC TTTATCCTGA
2281 TGATATTGCA AAAGCGCCTC ATGTTTTCCT TGAGAATTAC AAAACAGGTG TAACACATTC
2341 TTTCAATGAT CAACTGACGA TTACCTTGCG TGCAGATGCG AATACAACAA AAGCCGTTTA
2401 TCAAATCAAT AATGGACCAG ACGACAGGCG TTTAAGGATG GAGATCAATT CACAATCGGA
2461 AAAGGAGATC CAATTTGGCA AAACATACAC CATCATGTTA AAAGGAACGA ACAGTGATGG
2521 TGTAACGAGG ACCGAGAAAT ACAGTTTTGT TAAAAGAGAT CCAGCGTCGG CCAAAACCAT
2581 CGGCTATCAA AATCCGAATC ATTGGAGCCA GGTAAATGCT TATATCTATA AACATGATGG
2641 GAGCCGAGTA ATTGAATTGA CCGGATCTTG GCCTGGAAAA CCAATGACTA AAAATGCAGA
2701 CGGAATTTAC ACGCTGACGC TGCCTGCGGA CACGGATACA ACCAACGCAA AAGTGATTTT
2761 TAATAATGGC AGCGCCCAAG TGCCCGGTCA GAATCAGCCT GGCTTTGATT ACGTGCTAAA
2821 TGGTTTATAT AATGACTCGG GCTTAAGCGG TTCTCTTCCC CATTGAGGGC AAGGCTAGAC
2881 GGGACTTACC GAAAGAAACC ATCAATGATG GTTTCTTTTT TGTTCATAAA TCAGACAAAA
2941 CTTTTCTCTT GCAAAAGTTT GTGAAGTGTT GCACAATATA AATGTGAAAT ACTTCACAAA
3001 CAAAAAGACA TCAAAGAGAA ACATACCCTG CAAGGATGCT GATATTGTCT GCATTTGCGC
3061 CGGAGCAAAC CAAAAACCTG GTGAGACACG CCTTGAATTA GTAGAAAAGA ACTTGAAGAT
3121 TTTCAAAGGC ATCGTTAGTG AAGTCATGGC GAGCGGATTT GACGGCATTT TCTTAGTCGC
3181 GACGCGAGGC TGGATGGCCT TCCCCATTAT GATTCTTCTC GCTTCCGGCG GCATCGGGAT
3241 GCCCGCGTTG CAGGCCATGC TGTCCAGGCA GGTAGATGAC GACCATCAGG GACAGCTTCA
3301 AGGATCGCTC GCGGCTCTTA CCAGCCTAAC TTCGATCACT GGACCGCTGA TCGTCACGGC
3361 GATTTATGCC GCCTCGGCGA GCACATGGAA CGGGTTGGCA TGGATTGTAG GCGCCGCCCT
3421 ATACCTTGTC TGCCTCCCCG CGTTGCGTCG CGGTGCATGG AGCCGGGCCA CCTACTGAAG
3481 TGGATTTCTT TAAGAGCTCC TTTAACTTCC TCACCAGTAG TTGTATCGGT ACCATAAGTA
3541 GAAGCAGCAA CCCAAGTAGC TTTACCAGCA TCCGGTTCAA CCAGCATAGT AAGAATCTTA
3601 CTGGACATCG GCAGTTCTTC GAACAGTGCG CCAACTACCA GCTCTTTCTG CAGTTCATTC
3661 AGGGCACCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC
3721 CTGTCTCTTG ATCCATGGAT TACGCGTTAA CCCGGGCCCG CGGATGCATA TGATCAGATC
3781 CTTTAACTCT GGCAACCCTC AAAATTGAAT GAGACATGCT ACACCTCCGG ATAATAAATA
3841 TATATAAACG TATATAGATT TCATAAAGTC TAACACACTA GACTTATTTA CTTCGTAATT
3901 AAGTCGTTAA ACCGTGTGCT CTACGACCAA AACTATAAAA CCTTTAAGAA CTTTCTTTTT
3961 TTACAAGAAA AAAGAAATTA GATAAATCTC TCATATCTTT TATTCAATAA TCGCATCCGA
4021 TTGCAGTATA AATTTAACGA TCACTCATCA TGTTCATATT TATCAGAGCT CGTGCTATAA
4081 TTATACTAAT TTTATAAGGA GGAAAAAATA TGGGCATTTT TAGTATTTTT GTAATCAGCA
4141 CAGTTCATTA TCAACCAAAC AAAAAATAAG TGGTTATAAT GAATCGTTAA TAAGCAAAAT
4201 TCATATAACC AAATTAAAGA GGGTTATAAT GAACGAGAAA AATATAAAAC ACAGTCAAAA
4261 CTTTATTACT TCAAAACATA ATATAGATAA AATAATGACA AATATAAGAT TAAATGAACA
4321 TGATAATATC TTTGAAATCG GCTCAGGAAA AGGCCATTTT ACCCTTGAAT TAGTAAAGAG
4381 GTGTAATTTC GTAACTGCCA TTGAAATAGA CCATAAATTA TGCAAAACTA CAGAAAATAA
4441 ACTTGTTGAT CACGATAATT TCCAAGTTTT AAACAAGGAT ATATTGCAGT TTAAATTTCC
4501 TAAAAACCAA TCCTATAAAA TATATGGTAA TATACCTTAT AACATAAGTA CGGATATAAT
4561 ACGCAAAATT GTTTTTGATA GTATAGCTAA TGAGATTTAT TTAATCGTGG AATACGGGTT
4621 TGCTAAAAGA TTATTAAATA CAAAACGCTC ATTGGCATTA CTTTTAATGG CAGAAGTTGA
4681 TATTTCTATA TTAAGTATGG TTCCAAGAGA ATATTTTCAT CCTAAACCTA AAGTGAATAG
4741 CTCACTTATC AGATTAAGTA GAAAAAAATC AAGAATATCA CACAAAGATA AACAAAAGTA
4801 TAATTATTTC GTTATGAAAT GGGTTAACAA AGAATACAAG AAAATATTTA CAAAAAATCA
4861 ATTTAACAAT TCCTTAAAAC ATGCAGGAAT TGACGATTTA AACAATATTA GCTTTGAACA
4921 ATTCTTATCT CTTTTCAATA GCTATAAATT ATTTAATAAG TAAGTTAAGG GATGCATAAA
4981 CTGCATCCCT TAACTTGTTT TTCGTGTGCC TATTTTTTGT GAATCGATTA TGTCTTTTGC
5041 GCAGTCGGCT TAAACCAGTT TTCGCTGGTG CGAAAAAAGA GTGTCTTGTG ACACCTAAAT
5101 TCAAAATCTA TCGGTCAGAT TTATACCGAT TTGATTTTAT ATATTCTTGA ATAACATACG
5161 CCGAGTTATC ACATAAAAGC GGGAACCAAT CATCAAATTT AAACTTCATT GCATAATCCA
5221 TTAAACTCTT AAATTCTACG ATTCCTTGTT CATCAATAAA CTCAATCATT TCTTTAATTA
5281 ATTTATATCT ATCTGTTGTT GTTTTCTTTA ATAATTCATC AACATCTACA CCGCCATAAA
5341 CTATCATATC TTCTTTTTGA TATTTAAATT TATTAGGATC TTAAGGCCTA GGTCTAGAGT
5401 CTTTGTTTTG ACGCCATTAG CGTACGTAAC AATCCTCGTT AAAGGACAAG GACCTGAGCG
5461 GAAGTGTATC GTACAGTAGA CGGAGTATAC TAGTATAGTC TATAGTCCGT GGAATTATTA
5521 TATTTATCTC CGACGATATT CTCATCAGTG AAATCCAGCT GGAGTTCTTT AGCAAATTTT
5581 TTTATTAGCT GAACTTAGTA TTAGTGGCCA TACTCCTCCA ATCCAAAGCT ATTTAGAAAG
5641 ATTACTATAT CCTCAAACAG GCGGTAACCG GCCTCTTCAT CGGGAATGCG CGCGACCTTC
5701 AGCATCGCCG GCATGTCCCC CTGGCGGACG GGAAGTATCC AGCTCGAGGT CGGGCCGCGT
5761 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
5821 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT
5881 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
5941 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG
6001 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT
6061 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
6121 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA
6181 AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA
6241 AGCCAGTTAC CTTCGGAAAA AGAGTTGATA GCTCTTGATC CGGCAAACAA ACCACCGCTG
6301 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG
6361 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG
6421 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT
6481 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT
6541 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC
6601 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA
6661 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG
6721 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT
6781 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA
6841 TTGCTGCAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT
6901 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT
6961 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG
7021 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG
7081 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG
7141 CGTCAACACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA
7201 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT
7261 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT
7321 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT
7381 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA
7441 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT
7501 TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA
7561 AAAATAGGCG TATCACGAGG CCCTTTCGTC TTCAAGAATT
