抗体表达质粒

pFUSE-hIgG1e1-Fc2 哺乳细胞抗体表达载体人源

发布时间:2018-05-27 21:01:24 | 浏览次数：

测序及更多信息：https://pan.baidu.com/s/14eIwhRgVzoq35FQQ9eT5Qw 密码：oh99

采购地址：淘宝店铺地址

载体基本信息

出品公司:	Invitrogen
载体名称:	pFUSE-hIgG1e1-Fc2
质粒类型:	哺乳细胞，抗体表达载体
启动子:	hEF1-HTLV prom
表达水平:	高
克隆方法:	多克隆位点，限制性内切酶
载体大小:	4194 bp
5' 测序引物:	--
3' 测序引物:	EBV-Reverse: 5'-CAGCTTATAATGGTTACAAAT-3'
载体标签:	/
载体抗性:	Zeo 博来霉素
稳定性:	瞬表达 Transient
组成型:	组成型 Constitutive
病毒/非病毒:	非病毒

全序列

1 GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG
61 AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA
121 ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT
181 ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC
241 AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT ACCTGAGGCC
301 GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC TCCTGAACTG
361 CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT CCGGCGCTCC
421 CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG CTTGCTCAAC
481 TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG TGACCGGCGC
541 CTACCTGAGA TCACCGGCGA AGGAGGGCCA CCATGTACAG GATGCAACTC CTGTCTTGCA
601 TTGCACTAAG TCTTGCACTT GTCACGAATT CGATATCGGC CATGGTTAGA TCTGACAAAA
661 CTCACACATG CCCACCGTGC CCAGCACCTG AACTCCTGGG GGGACCGTCA GTCTTCCTCT
721 TCCCCCCAAA ACCCAAGGAC CAACTGATGA TCTCCCGGAC CCCTGAGGTC ACATGCGTGG
781 TGGTGGACGT GAGCCACGAA GACCCTGAGG TCAAGTTCAA CTGGTACGTG GACGGCGTGG
841 AGGTGCATAA TGCCAAGACA AAGCCGCGGG AGGAGCAGTA CAACAGCACG TACCGTGTGG
901 TCAGCGTCCT CACCGTCCTG CACCAGGACT GGCTGAATGG CAAGGAGTAC AAGTGCAAGG
961 TCTCCAACAA AGCCCTCCCA GCCCCCATCG AGAAAACCAT CTCCAAAGCC AAAGGGCAGC
1021 CCCGAGAACC ACAGGTGTAC ACCCTGCCCC CATCCCGGGA GGAGATGACC AAGAACCAGG
1081 TCAGCCTGAC CTGCCTGGTC AAAGGCTTCT ATCCCAGCGA CATCGCCGTG GAGTGGGAGA
1141 GCAATGGGCA GCCGGAGAAC AACTACAAGA CCACGCCTCC CGTGCTGGAC TCCGACGGCT
1201 CCTTCTTCCT CTACAGCAAG CTCACCGTGG ACAAGAGCAG GTGGCAGCAG GGGAACGTCT
1261 TCTCATGCTC CGTGCTGCAT GAGGCTCTGC ACAACCACTA CACGCAGAAG AGCCTCTCCC
1321 TGTCTCCGGG TAAATGAGTG CTAGCTGGCC AGACATGATA AGATACATTG ATGAGTTTGG
1381 ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT
1441 TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA
1501 TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA
1561 CAAATGTGGT ATGGAATTAA TTCTAAAATA CAGCATAGCA AAACTTTAAC CTCCAAATCA
1621 AGCCTCTACT TGAATCCTTT TCTGAGGGAT GAATAAGGCA TAGGCATCAG GGGCTGTTGC
1681 CAATGTGCAT TAGCTGTTTG CAGCCTCACC TTCTTTCATG GAGTTTAAGA TATAGTGTAT
1741 TTTCCCAAGG TTTGAACTAG CTCTTCATTT CTTTATGTTT TAAATGCACT GACCTCCCAC
1801 ATTCCCTTTT TAGTAAAATA TTCAGAAATA ATTTAAATAC ATCATTGCAA TGAAAATAAA
1861 TGTTTTTTAT TAGGCAGAAT CCAGATGCTC AAGGCCCTTC ATAATATCCC CCAGTTTAGT
1921 AGTTGGACTT AGGGAACAAA GGAACCTTTA ATAGAAATTG GACAGCAAGA AAGCGAGCTT
1981 CTAGCTTATC CTCAGTCCTG CTCCTCTGCC ACAAAGTGCA CGCAGTTGCC GGCCGGGTCG
2041 CGCAGGGCGA ACTCCCGCCC CCACGGCTGC TCGCCGATCT CGGTCATGGC CGGCCCGGAG
2101 GCGTCCCGGA AGTTCGTGGA CACGACCTCC GACCACTCGG CGTACAGCTC GTCCAGGCCG
2161 CGCACCCACA CCCAGGCCAG GGTGTTGTCC GGCACCACCT GGTCCTGGAC CGCGCTGATG
2221 AACAGGGTCA CGTCGTCCCG GACCACACCG GCGAAGTCGT CCTCCACGAA GTCCCGGGAG
2281 AACCCGAGCC GGTCGGTCCA GAACTCGACC GCTCCGGCGA CGTCGCGCGC GGTGAGCACC
2341 GGAACGGCAC TGGTCAACTT GGCCATGATG GCTCCTCCTG TCAGGAGAGG AAAGAGAAGA
2401 AGGTTAGTAC AATTGCTATA GTGAGTTGTA TTATACTATG CAGATATACT ATGCCAATGA
2461 TTAATTGTCA AACTAGGGCT GCAGGGTTCA TAGTGCCACT TTTCCTGCAC TGCCCCATCT
2521 CCTGCCCACC CTTTCCCAGG CATAGACAGT CAGTGACTTA CCAAACTCAC AGGAGGGAGA
2581 AGGCAGAAGC TTGAGACAGA CCCGCGGGAC CGCCGAACTG CGAGGGGACG TGGCTAGGGC
2641 GGCTTCTTTT ATGGTGCGCC GGCCCTCGGA GGCAGGGCGC TCGGGGAGGC CTAGCGGCCA
2701 ATCTGCGGTG GCAGGAGGCG GGGCCGAAGG CCGTGCCTGA CCAATCCGGA GCACATAGGA
2761 GTCTCAGCCC CCCGCCCCAA AGCAAGGGGA AGTCACGCGC CTGTAGCGCC AGCGTGTTGT
2821 GAAATGGGGG CTTGGGGGGG TTGGGGCCCT GACTAGTCAA AACAAACTCC CATTGACGTC
2881 AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA
2941 CTGCCAAAAC CGCATCATCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT
3001 AGGAAAGTCC CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT
3061 GACGTCAATA GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT
3121 TTACCGTAAA TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG TTACTATGGG
3181 AACATACGTC ATTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC
3241 ATTTACCGTA AGTTATGTAA CGCCTGCAGG TTAATTAAGA ACATGTGAGC AAAAGGCCAG
3301 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
3361 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
3421 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
3481 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
3541 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
3601 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
3661 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
3721 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
3781 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
3841 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
3901 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
3961 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGGCTAG TTAATTAACA
4021 TTTAAATCAG CGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT
4081 TGTGTGAATC GTAACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA AACAAACTAG
4141 CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC ATTTCTCTAT CGAA