测序及更多信息： https://pan.baidu.com/s/1FM6iku2JAhKW3QAmE9Hzmw?pwd=hedg
采购地址：淘宝店铺地址
pDD162 (Peft-3::Cas9 + Empty sgRNA)
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骨架载体
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pCFJ601
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拷贝数
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高拷贝
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载体名称:
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pDD162 (Peft-3::Cas9 + Empty sgRNA)，蠕虫表达载体CRISPR相关载体
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质粒类型:
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CRISPR/Cas9载体；基因敲除载体
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克隆方法:
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限制性内切酶，多克隆位点
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启动子:
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eef-1A.1 (eft-3)
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载体大小:
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8113 bp
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5' 测序引物及序列:
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根据载体序列
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3' 测序引物及序列:
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根据载体序列
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载体标签:
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HA，NLS
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载体抗性:
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Amp
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克隆菌株:
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Stbl3
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备注:
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Worm Expression
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产品目录号:
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#47549
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全序列
1 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA
61 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC
121 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC
181 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA
241 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG
301 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA
361 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA
421 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC
481 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA
541 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA
601 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG
661 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA
721 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT
781 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT
841 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG
901 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA
961 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC
1021 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
1081 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
1141 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC
1201 GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG
1261 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA
1321 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
1381 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC
1441 TTTTTACGGT TCCTGGTCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC
1501 CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCAG CGGATAACAA
1561 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC TATCAACTTT GTATAGAAAA
1621 GTTGGCACCT TTGGTCTTTT ATTGTCAACT TCCATTGGTT CTTCCATTGT TTCTGTTAAA
1681 TTAATGAATT TTTCATAAAA TAAAGACATT ATACAATATA AAAATGAAGA ATTTATTGAA
1741 AATAAACTGC CAGAGAGAAA AAGTATGCAA CACTCCCGCC GAGAGTGTTT GAAATGGTGT
1801 ACGGTACATT TTCGTGCTAG GAGTTAGATG TGCAGGCAGC AACGAGAGGG GGAGAGATTT
1861 TTTTGGGCCT TGTGAAATTA ACGTGAGTTT TCTGGTCATC TGACTAATCA TGTTGGTTTT
1921 TTGTTGGTTT ATTTTGTTTT TATCTTTGTT TTTATCCAGA TTAGGAAATT TAAATTTTAT
1981 GAATTTATAA TGAGGTCAAA CATTCAGTCC CAGCGTTTTT CCTGTTCTCA CTGTTTAGTC
2041 GAATTTTTAT TTTAGGCTTT CAACAAATGT TCTAACTGTC TTATTTGTGA CCTCACTTTT
2101 TATATTTTTT TAATTTTTAA AAATATTAGA AGTTTCTAGG ATAATTTTTT CGACTTTTAT
2161 TCTCTCTACC GTCCGCACTC TTCTTACTTT TAAATTAAAT TGTTTTTTTT TCAGTTGGGA
2221 AACACTTTGC TCTAGAAAAA ATGGACAAAA AATACAGCAT CGGCCTTGAC ATCGGAACGA
2281 ACTCCGTCGG ATGGGCCGTC ATCACCGACG AGTACAAGGT CCCATCCAAG AAGTTCAAGG
2341 TCCTCGGAAA CACCGACCGT CACTCCATCA AGAAGAACCT CATCGGAGCC CTCCTCTTCG
2401 ACTCCGGAGA GACCGCCGAG GCCACCCGTC TCAAGCGTAC CGCCCGTCGT CGTTACACCC
2461 GTCGTAAGAA CCGTATCTGC TACCTCCAAG AGATCTTCTC CAACGAGATG GCCAAGGTCG
2521 ACGACTCCTT CTTCCACCGT CTCGAGGAGT CCTTCCTCGT CGAGGAGGAC AAGAAGCACG
2581 AGCGTCACCC AATCTTCGGA AACATCGTCG ACGAGGTCGC CTACCACGAG AAGTACCCAA
2641 CCATCTACCA CCTCCGTAAG AAGCTCGTCG ACTCCACCGA CAAGGCCGAC CTCCGTCTCA
2701 TCTACCTCGC CCTCGCCCAC ATGATCAAGT TCCGTGGACA CTTCCTCATC GAGGGAGACC
2761 TCAACCCAGA CAACTCCGAC GTCGACAAGC TCTTCATCCA ACTCGTCCAA ACCTACAACC
2821 AACTCTTCGA GGAGAACCCA ATCAACGCCT CCGGAGTCGA CGCCAAGGCC ATCCTCTCCG
2881 CCCGTCTCTC CAAGTCCCGT CGTCTCGAGA ACCTCATCGC CCAACTCCCA GGAGAGAAGA
2941 AGAACGGACT CTTCGGAAAC CTCATCGCCC TCTCCCTCGG ACTCACCCCA AACTTCAAGT
3001 CCAACTTCGA CCTCGCCGAG GACGCCAAGC TCCAACTCTC CAAGGACACC TACGACGACG
3061 ACCTCGACAA CCTCCTCGCC CAAATCGGAG ACCAATACGC CGACCTCTTC CTCGCCGCCA
3121 AGAACCTCTC CGACGCCATC CTCCTCTCCG ACATCCTCCG TGTCAACACC GAGATCACCA
3181 AGGCCCCACT CTCCGCCTCC ATGATCAAGC GTTACGACGA GCACCACCAA GACCTCACCC
3241 TCCTCAAGGC CCTCGTCCGT CAACAACTCC CAGAGAAGTA CAAGGTAAGT TTAAACATAT
3301 ATATACTAAC TAACCCTGAT TATTTAAATT TTCAGGAGAT CTTCTTCGAC CAATCCAAGA
3361 ACGGATACGC CGGATACATC GACGGAGGAG CCTCCCAAGA GGAGTTCTAC AAGTTCATCA
3421 AGCCAATCCT CGAGAAGATG GACGGAACCG AGGAGCTCCT CGTCAAGCTC AACCGTGAGG
3481 ACCTCCTCCG TAAGCAACGT ACCTTCGACA ACGGATCCAT CCCACACCAA ATCCACCTCG
3541 GAGAGCTCCA CGCCATCCTC CGTCGTCAAG AGGACTTCTA CCCATTCCTC AAGGACAACC
3601 GTGAGAAGAT CGAGAAGATC CTCACCTTCC GTATCCCATA CTACGTCGGA CCACTCGCCC
3661 GTGGAAACTC CCGTTTCGCC TGGATGACCC GTAAGTCCGA GGAGACCATC ACCCCATGGA
3721 ACTTCGAGGA GGTCGTCGAC AAGGGAGCCT CCGCCCAATC CTTCATCGAG CGTATGACCA
3781 ACTTCGACAA GAACCTCCCA AACGAGAAGG TCCTCCCAAA GCACTCCCTC CTCTACGAGT
3841 ACTTCACCGT CTACAACGAG CTCACCAAGG TCAAGTACGT CACCGAGGGA ATGCGTAAGC
3901 CAGCCTTCCT CTCCGGAGAG CAAAAGAAGG CCATCGTCGA CCTCCTCTTC AAGACCAACC
3961 GTAAGGTCAC CGTCAAGCAA CTCAAGGAGG ACTACTTCAA GAAGATCGAG TGCTTCGACT
4021 CCGTCGAGAT CTCCGGAGTC GAGGACCGTT TCAACGCCTC CCTCGGAACC TACCACGACC
4081 TCCTCAAGAT CATCAAGGAC AAGGACTTCC TCGACAACGA GGAGAACGAG GACATCCTCG
4141 AGGACATCGT CCTCACCCTC ACCCTCTTCG AGGACCGTGA GATGATCGAG GAGCGTCTCA
4201 AGACCTACGC CCACCTCTTC GACGACAAGG TCATGAAGCA GCTTAAGCGT CGTCGTTACA
4261 CCGGATGGGG ACGTCTCTCC CGTAAGCTCA TCAACGGAAT CCGTGACAAG CAATCCGGAA
4321 AGACCATCCT CGACTTCCTC AAGTCCGACG GATTCGCCAA CCGTAACTTC ATGCAACTCA
4381 TCCACGACGA CTCCCTCACC TTCAAGGTAA GTTTAAACAG TTCGGTACTA ACTAACCATA
4441 CATATTTAAA TTTTCAGGAG GACATCCAAA AGGCCCAAGT CTCCGGACAA GGAGACTCCC
4501 TCCACGAGCA CATCGCCAAC CTCGCCGGAT CCCCAGCCAT CAAGAAGGGA ATCCTCCAAA
4561 CCGTCAAGGT CGTCGACGAG CTCGTCAAGG TCATGGGACG TCACAAGCCA GAGAACATCG
4621 TCATCGAGAT GGCCCGTGAG AACCAAACCA CCCAAAAGGG ACAAAAGAAC TCCCGTGAGC
4681 GTATGAAGCG TATCGAGGAG GGAATCAAGG AGCTCGGATC CCAAATCCTC AAGGAGCACC
4741 CAGTCGAGAA CACCCAACTC CAAAACGAGA AGCTCTACCT CTACTACCTC CAAAACGGAC
4801 GTGACATGTA CGTCGACCAA GAGCTCGACA TCAACCGTCT CTCCGACTAC GACGTCGACC
4861 ACATCGTCCC ACAATCCTTC CTCAAGGACG ACTCCATCGA CAACAAGGTC CTCACCCGTT
4921 CCGACAAGAA CCGTGGAAAG TCCGACAACG TCCCATCCGA GGAGGTCGTC AAGAAGATGA
4981 AGAACTACTG GCGTCAACTC CTCAACGCCA AGCTCATCAC CCAACGTAAG TTCGACAACC
5041 TCACCAAGGC TGAGCGTGGA GGACTCTCCG AGCTCGACAA GGCCGGATTC ATCAAGCGTC
5101 AACTCGTCGA GACCCGTCAA ATCACCAAGC ACGTCGCCCA AATCCTCGAC TCCCGTATGA
5161 ACACCAAGTA CGACGAGAAC GACAAGCTCA TCCGTGAGGT CAAGGTCATC ACCCTCAAGT
5221 CCAAGCTCGT CTCCGACTTC CGTAAGGACT TCCAATTCTA CAAGGTCCGT GAGATCAACA
5281 ACTACCACCA CGCCCACGAC GCCTACCTCA ACGCCGTAGT AGGAACCGCC CTCATCAAGA
5341 AGTACCCAAA GCTCGAGTCC GAGTTCGTCT ACGGAGACTA CAAGGTCTAC GACGTCCGTA
5401 AGATGATCGC CAAGTCCGAG CAAGAGATCG GAAAGGCCAC CGCCAAGTAC TTCTTCTACT
5461 CCAACATCAT GAACTTCTTC AAGGTAAGTT TAAACATGAT TTTACTAACT AACTAATCTG
5521 ATTTAAATTT TCAGACCGAG ATCACCCTCG CCAACGGAGA GATCCGTAAG CGTCCACTCA
5581 TCGAGACCAA CGGAGAGACC GGAGAGATCG TCTGGGACAA GGGACGTGAC TTCGCCACAG
5641 TCCGTAAGGT CCTCTCCATG CCACAAGTCA ACATCGTCAA GAAGACCGAG GTCCAAACCG
5701 GAGGATTCTC CAAGGAGTCC ATCCTCCCAA AGCGTAACTC CGACAAGCTC ATCGCCCGTA
5761 AGAAGGACTG GGACCCAAAG AAGTACGGAG GATTCGACTC CCCAACCGTC GCCTACTCCG
5821 TCCTCGTCGT CGCCAAGGTC GAGAAGGGAA AGTCCAAGAA GCTCAAGTCC GTCAAGGAGC
5881 TCCTCGGAAT CACCATCATG GAGCGTTCCT CCTTCGAGAA GAACCCAATC GACTTCCTCG
5941 AGGCCAAGGG ATACAAGGAG GTCAAGAAGG ACCTCATCAT CAAGCTCCCA AAGTACTCCC
6001 TCTTCGAGCT CGAGAACGGA CGTAAGCGTA TGCTCGCCTC CGCCGGAGAG CTCCAAAAGG
6061 GAAACGAGCT CGCCCTCCCA TCCAAGTACG TCAACTTCCT CTACCTCGCC TCCCACTACG
6121 AGAAGCTCAA GGGATCCCCA GAGGACAACG AGCAAAAGCA ACTCTTCGTC GAGCAACACA
6181 AGCACTACCT CGACGAGATC ATCGAGCAAA TCTCCGAGTT CTCCAAGCGT GTCATCCTCG
6241 CCGACGCCAA CCTCGACAAG GTCCTCTCCG CCTACAACAA GCACCGTGAC AAGCCAATCC
6301 GTGAGCAAGC CGAGAACATC ATCCACCTCT TCACCCTCAC CAACCTCGGA GCCCCAGCCG
6361 CCTTCAAGTA CTTCGACACC ACCATCGACC GTAAGCGTTA CACCTCCACC AAGGAGGTCC
6421 TCGACGCCAC CCTCATCCAC CAATCCATCA CCGGACTCTA CGAGACCCGT ATCGACCTCT
6481 CCCAACTCGG AGGAGACGGA GGATCCGGAC CACCAAAGAA GAAGCGTAAG GTCTACCCAT
6541 ACGACGTCCC AGACTACGCC TAAACTAGTG ATAAATGCAA AATCCTTTCA AGCATTCCCT
6601 TCTTCTCTAT CACTCTTCTT TCTTTTTGTC AAAAAATTCT CTCGCTAATT TATTTGCTTT
6661 TTTAATGTTA TTATTTTATG ACTTTTTATA GTCACTGAAA AGTTTGCATC TGAGTGAAGT
6721 GAATGCTATC AAAATGTGAT TCTGTCTGAT GTACTTTCAC AATCTCTCTT CAATTCCATT
6781 TTGAAGTGCT TTAAACCCGA AAGGTTGAGA AAAATGCGAG CGCTCAAATA TTTGTATTGT
6841 GTTCGTTGAG TGACCCAACA AAAAGAGGAA ACTTTATTGT GCCGCCAAGA AAAAAGTCTC
6901 ACAACTTTAT TATACATAGT TGATAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG
6961 GAAAACCAAA AAAAACTAGC AATAAAGGAA TAAAAAACTG TACACCTTAA AGGCGCACAC
7021 TCTGTTTTGC AAATTTTATT TTTAGTTGTG AATTTTCTGC TGAGACCTGA AAATAGCAAC
7081 TTTAGTACTA CTATAATTTG TCAACCTTTT CAAAAAAAGC ATGCAATTTT TGAGAAACTC
7141 TTATAAAAGC TATTATTAAA AAAACACCTT TTTTCCAAAA TTATTCCACA AAAAATATGT
7201 TATGAAATGC CTACACCCTC TCACACACAC TCTTTATACT ACTCTGTCAA ACTCACGAGA
7261 TGTCTGCCGC CTCTTGTGTT GCCCCTATAT AAACACCTCC TATTGCGAGA TGTCTTGGTT
7321 TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC
7381 ACCGAGTCGG TGCTTTTTGT GAAATTTCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG
7441 CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC CTGATGCGGT ATTTTCTCCT
7501 TACGCATCTG TGCGGTATTT CACACCGCAT ATGGTGCACT CTCAGTGCAA TCTGCTCTGA
7561 TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC
7621 TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG
7681 TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT
7741 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG
7801 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC
7861 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG
7921 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT
7981 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT
8041 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA
8101 ACGTTTTCCA ATG
pDD162 (Peft-3 Cas9 + Empty sgRNA)(Plasmid #47549)
pDD162 (Peft-3 Cas9 + Empty sgRNA)(Plasmid #47549)
pDD162 (Peft-3 Cas9 + Empty sgRNA)(Plasmid #47549)
