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原核编辑质粒

pTargetF 大肠杆菌编辑质粒 CRISPR/Cas9载体 需配合pCAS使用
发布时间:2019-02-09 13:04:42 | 浏览次数:


测序及更多信息:https://pan.baidu.com/s/16daYQrz_iCaF_gwgj9MhJw 密码:228a

采购地址:淘宝店铺地址

PURPOSE

Constitutive expression of sgRNA without donor editing template DNA

BACKBONE

  • Vector backbone

Trc99a

 (Search Vector Database)

  • Backbone size w/o insert (bp)2000
  • Modifications to backbone

Replaced Amp with aadA

  • Vector type

Bacterial Expression, CRISPR

GROWTH IN BACTERIA

  • Bacterial Resistance(s)

Spectinomycin

  • Growth Temperature

37°C

  • Growth Strain(s)

DH5alpha

  • Copy number

High Copy

 

  • Gene/Insert name

sgRNA

  • gRNA/shRNA sequence

GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTG

  • Species

Synthetic

  • Promoterpij23119

CLONING INFORMATION

  • Cloning methodRestriction Enzyme
  • 5′ cloning siteunknown (unknown if destroyed)
  • 3′ cloning siteunknown (unknown if destroyed)
  • 5′ sequencing primerMoClo-F (agcgaggaagcggaagagcg)


全序列

1        TCGAGTTCAT GTGCAGCTCC ATAAGCAAAA GGGGATGATA AGTTTATCAC CACCGACTAT
61       TTGCAACAGT GCCGTTGATC GTGCTATGAT CGACTGATGT CATCAGCGGT GGAGTGCAAT
121      GTCATGAGGG AAGCGGTGAT CGCCGAAGTA TCGACTCAAC TATCAGAGGT AGTTGGCGTC
181      ATCGAGCGCC ATCTCGAACC GACGTTGCTG GCCGTACATT TGTACGGCTC CGCAGTGGAT
241      GGCGGCCTGA AGCCACACAG TGATATTGAT TTGCTGGTTA CGGTGACCGT AAGGCTTGAT
301      GAAACAACGC GGCGAGCTTT GATCAACGAC CTTTTGGAAA CTTCGGCTTC CCCTGGAGAG
361      AGCGAGATTC TCCGCGCTGT AGAAGTCACC ATTGTTGTGC ACGACGACAT CATTCCGTGG
421      CGTTATCCAG CTAAGCGCGA ACTGCAATTT GGAGAATGGC AGCGCAATGA CATTCTTGCA
481      GGTATCTTCG AGCCAGCCAC GATCGACATT GATCTGGCTA TCTTGCTGAC AAAAGCAAGA
541      GAACATAGCG TTGCCTTGGT AGGTCCAGCG GCGGAGGAAC TCTTTGATCC GGTTCCTGAA
601      CAGGATCTAT TTGAGGCGCT AAATGAAACC TTAACGCTAT GGAACTCGCC GCCCGACTGG
661      GCTGGCGATG AGCGAAATGT AGTGCTTACG TTGTCCCGCA TTTGGTACAG CGCAGTAACC
721      GGCAAAATCG CGCCGAAGGA TGTCGCTGCC GACTGGGCAA TGGAGCGCCT GCCGGCCCAG
781      TATCAGCCCG TCATACTTGA AGCTAGACAG GCTTATCTTG GACAAGAAGA AGATCGCTTG
841      GCCTCGCGCG CAGATCAGTT GGAAGAATTT GTCCACTACG TGAAAGGCGA GATCACCAAG
901      GTAGTCGGCA AATAAGATGC CGCTCGCCAG TCGATTGGCT GAGCTCATGA AGTTCCTATT
961      CCGAAGTTCC GCGAACGCGT AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC
1021     CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA
1081     AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC
1141     ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT
1201     AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG
1261     CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC
1321     AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT
1381     ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA
1441     GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT
1501     TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG
1561     CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA
1621     CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA
1681     CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT
1741     CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
1801     TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
1861     GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGCTG
1921     GATCCTTGAC AGCTAGCTCA GTCCTAGGTA TAATACTAGT CATCGCCGCA GCGGTTTCAG
1981     GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC CGTTATCAAC TTGAAAAAGT
2041     GGCACCGAGT CGGTGCTTTT TTTGAATTCT CTAGAGTCGA CCTGCAGAAG CTTAGATCTA
2101     TTACCCTGTT ATCCCTAC





 
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