pBUE411
(Plasmid #62200)
测序和更多信息: https://pan.baidu.com/s/1FsvFhY2rY8C9dqKpsHzo_g 密码: u54s
采购地址：淘宝店铺地址
载体作用
CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
载体骨架
pCambia
构建方法
载体类型
植物表达载体 plant binary vector
筛选标记
Basta
插入基因信息
1，gRNA scaffold；
2，玉米密码子优化的S. pyogenes zCas9基因。
参考文献
A CRISPR/Cas9 toolkit for multiplex genome editing in plants. Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ. BMC Plant Biol. 2014 Nov 29;14(1):327. 10.1186/s12870-014-0327-y PubMed 25432517
全序列
1 TAAACGCTCT TTTCTCTTAG GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA
61 AACTGAAGGC GGGAAACGAC AATCTGATCC AAGCTCAAGC TGCTCTAGCA TTCGCCATTC
121 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG
181 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
241 CGACGTTGTA AAACGACGGC CAGTGCCAAG CTTAGTAATT CATCCAGGTC ACCAAGTTCT
301 AGGATTTTCA GAACTGCAAC TTATTTTATC AAGGAATCTT TAAACATACG AACAGATCAC
361 TTAAAGTTCT TCTGAAGCAA CTTAAAGTTA TCAGGCATGC ATGGATCTTG GAGGAATCAG
421 ATGTGCAGTC AGGGACCATA GCACAAGACA GGCGTCTTCT ACTGGTGCTA CCAGCAAATG
481 CTGGAAGCCG GGAACACTGG GTACGTTGGA AACCACGTGA TGTGAAGAAG TAAGATAAAC
541 TGTAGGAGAA AAGCATTTCG TAGTGGGCCA TGAAGCCTTT CAGGACATGT ATTGCAGTAT
601 GGGCCGGCCC ATTACGCAAT TGGACGACAA CAAAGACTAG TATTAGTACC ACCTCGGCTA
661 TCCACATAGA TCAAAGCTGA TTTAAAAGAG TTGTGCAGAT GATCCGTGGC GTGAGACCAA
721 CCCAGTGGAC ATAAGCCTGT TCGGTTCGTA AGCTGTAATG CAAGTAGCGT ATGCGCTCAC
781 GCAACTGGTC CAGAACCTTG ACCGAACGCA GCGGTGGTAA CGGCGCAGTG GCGGTTTTCA
841 TGGCTTGTTA TGACTGTTTT TTTGGGGTAC AGTCTATGCC TCGGGCATCC AAGCAGCAAG
901 CGCGTTACGC CGTGGGTCGA TGTTTGATGT TATGGAGCAG CAACGATGTT ACGCAGCAGG
961 GCAGTCGCCC TAAAACAAAG TTAAACATCA TGGGGGAAGC GGTGATCGCC GAAGTATCGA
1021 CTCAACTATC AGAGGTAGTT GGCGTCATCG AGCGCCATCT CGAACCGACG TTGCTGGCCG
1081 TACATTTGTA CGGCTCCGCA GTGGATGGCG GCCTGAAGCC ACACAGTGAT ATTGATTTGC
1141 TGGTTACGGT GACCGTAAGG CTTGATGAAA CAACGCGGCG AGCTTTGATC AACGACCTTT
1201 TGGAAACTTC GGCTTCCCCT GGAGAGAGCG AGATTCTCCG CGCTGTAGAA GTCACCATTG
1261 TTGTGCACGA CGACATCATT CCGTGGCGTT ATCCAGCTAA GCGCGAACTG CAATTTGGAG
1321 AATGGCAGCG CAATGACATT CTTGCAGGTA TCTTCGAGCC AGCCACGATC GACATTGATC
1381 TGGCTATCTT GCTGACAAAA GCAAGAGAAC ATAGCGTTGC CTTGGTAGGT CCAGCGGCGG
1441 AGGAACTCTT TGATCCGGTT CCTGAACAGG ATCTATTTGA GGCGCTAAAT GAAACCTTAA
1501 CGCTATGGAA CTCGCCGCCC GACTGGGCTG GCGATGAGCG AAATGTAGTG CTTACGTTGT
1561 CCCGCATTTG GTACAGCGCA GTAACCGGCA AAATCGCGCC GAAGGATGTC GCTGCCGACT
1621 GGGCAATGGA GCGCCTGCCG GCCCAGTATC AGCCCGTCAT ACTTGAAGCT AGACAGGCTT
1681 ATCTTGGACA AGAAGAAGAT CGCTTGGCCT CGCGCGCAGA TCAGTTGGAA GAATTTGTCC
1741 ACTACGTGAA AGGCGAGATC ACCAAGGTAG TCGGCAAATA ATGTCTAGCT AGAAATTCGT
1801 TCAAGCCGAC GCCGCTTCGC GGCGCGGCTT AACTCAAGCG TTAGATGCAC TAAGCACATA
1861 ATTGCTCACA GCCAAACTAT CAGGTCAAGT CTGCTTTTAT TATTTTTAAG CGTGCATAAT
1921 AAGCCGGTCT CGGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTATCA
1981 ACTTGAAAAA GTGGCACCGA GTCGGTGCTT TTTTTTTTCG TTTTGCATTG AGTTTTCTCC
2041 GTCGCATGTT TGCAGTTTTA TTTTCCGTTT TGCATTGAAA TTTCTCCGTC TCATGTTTGC
2101 AGCGTGTTCA AAAAGTACGC AGCTGTATTT CACTTATTTA CGGCGCCACA TTTTCATGCC
2161 GTTTGTGCCA ACTATCCCGA GCTAGTGAAT ACAGCTTGGC TTCACACAAC ACTGGTGACC
2221 CGCTGACCTG CTCGTACCTC GTACCGTCGT ACGGCACAGC ATTTGGAATT AAAGGGTGTG
2281 ATCGATACTG CTTGCTGCTA AGCTTGCATG CCTGCAGTGC AGCGTGACCC GGTCGTGCCC
2341 CTCTCTAGAG ATAATGAGCA TTGCATGTCT AAGTTATAAA AAATTACCAC ATATTTTTTT
2401 TGTCACACTT GTTTGAAGTG CAGTTTATCT ATCTTTATAC ATATATTTAA ACTTTACTCT
2461 ACGAATAATA TAATCTATAG TACTACAATA ATATCAGTGT TTTAGAGAAT CATATAAATG
2521 AACAGTTAGA CATGGTCTAA AGGACAATTG AGTATTTTGA CAACAGGACT CTACAGTTTT
2581 ATCTTTTTAG TGTGCATGTG TTCTCCTTTT TTTTTGCAAA TAGCTTCACC TATATAATAC
2641 TTCATCCATT TTATTAGTAC ATCCATTTAG GGTTTAGGGT TAATGGTTTT TATAGACTAA
2701 TTTTTTTAGT ACATCTATTT TATTCTATTT TAGCCTCTAA ATTAAGAAAA CTAAAACTCT
2761 ATTTTAGTTT TTTTATTTAA TAATTTAGAT ATAAAATAGA ATAAAATAAA GTGACTAAAA
2821 ATTAAACAAA TACCCTTTAA GAAATTAAAA AAACTAAGGA AACATTTTTC TTGTTTCGAG
2881 TAGATAATGC CAGCCTGTTA AACGCCGTCG ACGAGTCTAA CGGACACCAA CCAGCGAACC
2941 AGCAGCGTCG CGTCGGGCCA AGCGAAGCAG ACGGCACGGC ATCTCTGTCG CTGCCTCTGG
3001 ACCCCTCTCG AGAGTTCCGC TCCACCGTTG GACTTGCTCC GCTGTCGGCA TCCAGAAATT
3061 GCGTGGCGGA GCGGCAGACG TGAGCCGGCA CGGCAGGCGG CCTCCTCCTC CTCTCACGGC
3121 ACGGCAGCTA CGGGGGATTC CTTTCCCACC GCTCCTTCGC TTTCCCTTCC TCGCCCGCCG
3181 TAATAAATAG ACACCCCCTC CACACCCTCT TTCCCCAACC TCGTGTTGTT CGGAGCGCAC
3241 ACACACACAA CCAGATCTCC CCCAAATCCA CCCGTCGGCA CCTCCGCTTC AAGGTACGCC
3301 GCTCGTCCTC CCCCCCCCCC CCTCTCTACC TTCTCTAGAT CGGCGTTCCG GTCCATGGTT
3361 AGGGCCCGGT AGTTCTACTT CTGTTCATGT TTGTGTTAGA TCCGTGTTTG TGTTAGATCC
3421 GTGCTGCTAG CGTTCGTACA CGGATGCGAC CTGTACGTCA GACACGTTCT GATTGCTAAC
3481 TTGCCAGTGT TTCTCTTTGG GGAATCCTGG GATGGCTCTA GCCGTTCCGC AGACGGGATC
3541 GATTTCATGA TTTTTTTTGT TTCGTTGCAT AGGGTTTGGT TTGCCCTTTT CCTTTATTTC
3601 AATATATGCC GTGCACTTGT TTGTCGGGTC ATCTTTTCAT GCTTTTTTTT GTCTTGGTTG
3661 TGATGATGTG GTCTGGTTGG GCGGTCGTTC TAGATCGGAG TAGAATTCTG TTTCAAACTA
3721 CCTGGTGGAT TTATTAATTT TGGATCTGTA TGTGTGTGCC ATACATATTC ATAGTTACGA
3781 ATTGAAGATG ATGGATGGAA ATATCGATCT AGGATAGGTA TACATGTTGA TGCGGGTTTT
3841 ACTGATGCAT ATACAGAGAT GCTTTTTGTT CGCTTGGTTG TGATGATGTG GTGTGGTTGG
3901 GCGGTCGTTC ATTCGTTCTA GATCGGAGTA GAATACTGTT TCAAACTACC TGGTGTATTT
3961 ATTAATTTTG GAACTGTATG TGTGTGTCAT ACATCTTCAT AGTTACGAGT TTAAGATGGA
4021 TGGAAATATC GATCTAGGAT AGGTATACAT GTTGATGTGG GTTTTACTGA TGCATATACA
4081 TGATGGCATA TGCAGCATCT ATTCATATGC TCTAACCTTG AGTACCTATC TATTATAATA
4141 AACAAGTATG TTTTATAATT ATTTTGATCT TGATATACTT GGATGATGGC ATATGCAGCA
4201 GCTATATGTG GATTTTTTTA GCCCTGCCTT CATACGCTAT TTATTTGCTT GGTACTGTTT
4261 CTTTTGTCGA TGCTCACCCT GTTGTTTGGT GTTACTTCTG CAGCCCTAGG CCTACTAGAT
4321 GGATTACAAG GACCACGACG GGGATTACAA GGACCACGAC ATTGATTACA AGGATGATGA
4381 TGACAAGATG GCTCCGAAGA AGAAGAGGAA GGTTGGCATC CACGGGGTGC CAGCTGCTGA
4441 CAAGAAGTAC TCGATCGGCC TCGATATTGG GACTAACTCT GTTGGCTGGG CCGTGATCAC
4501 CGACGAGTAC AAGGTGCCCT CAAAGAAGTT CAAGGTCCTG GGCAACACCG ATCGGCATTC
4561 CATCAAGAAG AATCTCATTG GCGCTCTCCT GTTCGACAGC GGCGAGACGG CTGAGGCTAC
4621 GCGGCTCAAG CGCACCGCCC GCAGGCGGTA CACGCGCAGG AAGAATCGCA TCTGCTACCT
4681 GCAGGAGATT TTCTCCAACG AGATGGCGAA GGTTGACGAT TCTTTCTTCC ACAGGCTGGA
4741 GGAGTCATTC CTCGTGGAGG AGGATAAGAA GCACGAGCGG CATCCAATCT TCGGCAACAT
4801 TGTCGACGAG GTTGCCTACC ACGAGAAGTA CCCTACGATC TACCATCTGC GGAAGAAGCT
4861 CGTGGACTCC ACAGATAAGG CGGACCTCCG CCTGATCTAC CTCGCTCTGG CCCACATGAT
4921 TAAGTTCAGG GGCCATTTCC TGATCGAGGG GGATCTCAAC CCGGACAATA GCGATGTTGA
4981 CAAGCTGTTC ATCCAGCTCG TGCAGACGTA CAACCAGCTC TTCGAGGAGA ACCCCATTAA
5041 TGCGTCAGGC GTCGACGCGA AGGCTATCCT GTCCGCTAGG CTCTCGAAGT CTCGGCGCCT
5101 CGAGAACCTG ATCGCCCAGC TGCCGGGCGA GAAGAAGAAC GGCCTGTTCG GGAATCTCAT
5161 TGCGCTCAGC CTGGGGCTCA CGCCCAACTT CAAGTCGAAT TTCGATCTCG CTGAGGACGC
5221 CAAGCTGCAG CTCTCCAAGG ACACATACGA CGATGACCTG GATAACCTCC TGGCCCAGAT
5281 CGGCGATCAG TACGCGGACC TGTTCCTCGC TGCCAAGAAT CTGTCGGACG CCATCCTCCT
5341 GTCTGATATT CTCAGGGTGA ACACCGAGAT TACGAAGGCT CCGCTCTCAG CCTCCATGAT
5401 CAAGCGCTAC GACGAGCACC ATCAGGATCT GACCCTCCTG AAGGCGCTGG TCAGGCAGCA
5461 GCTCCCCGAG AAGTACAAGG AGATCTTCTT CGATCAGTCG AAGAACGGCT ACGCTGGGTA
5521 CATTGACGGC GGGGCCTCTC AGGAGGAGTT CTACAAGTTC ATCAAGCCGA TTCTGGAGAA
5581 GATGGACGGC ACGGAGGAGC TGCTGGTGAA GCTCAATCGC GAGGACCTCC TGAGGAAGCA
5641 GCGGACATTC GATAACGGCA GCATCCCACA CCAGATTCAT CTCGGGGAGC TGCACGCTAT
5701 CCTGAGGAGG CAGGAGGACT TCTACCCTTT CCTCAAGGAT AACCGCGAGA AGATCGAGAA
5761 GATTCTGACT TTCAGGATCC CGTACTACGT CGGCCCACTC GCTAGGGGCA ACTCCCGCTT
5821 CGCTTGGATG ACCCGCAAGT CAGAGGAGAC GATCACGCCG TGGAACTTCG AGGAGGTGGT
5881 CGACAAGGGC GCTAGCGCTC AGTCGTTCAT CGAGAGGATG ACGAATTTCG ACAAGAACCT
5941 GCCAAATGAG AAGGTGCTCC CTAAGCACTC GCTCCTGTAC GAGTACTTCA CAGTCTACAA
6001 CGAGCTGACT AAGGTGAAGT ATGTGACCGA GGGCATGAGG AAGCCGGCTT TCCTGTCTGG
6061 GGAGCAGAAG AAGGCCATCG TGGACCTCCT GTTCAAGACC AACCGGAAGG TCACGGTTAA
6121 GCAGCTCAAG GAGGACTACT TCAAGAAGAT TGAGTGCTTC GATTCGGTCG AGATCTCTGG
6181 CGTTGAGGAC CGCTTCAACG CCTCCCTGGG GACCTACCAC GATCTCCTGA AGATCATTAA
6241 GGATAAGGAC TTCCTGGACA ACGAGGAGAA TGAGGATATC CTCGAGGACA TTGTGCTGAC
6301 ACTCACTCTG TTCGAGGACC GGGAGATGAT CGAGGAGCGC CTGAAGACTT ACGCCCATCT
6361 CTTCGATGAC AAGGTCATGA AGCAGCTCAA GAGGAGGAGG TACACCGGCT GGGGGAGGCT
6421 GAGCAGGAAG CTCATCAACG GCATTCGGGA CAAGCAGTCC GGGAAGACGA TCCTCGACTT
6481 CCTGAAGAGC GATGGCTTCG CGAACCGCAA TTTCATGCAG CTGATTCACG ATGACAGCCT
6541 CACATTCAAG GAGGATATCC AGAAGGCTCA GGTGAGCGGC CAGGGGGACT CGCTGCACGA
6601 GCATATCGCG AACCTCGCTG GCTCGCCAGC TATCAAGAAG GGGATTCTGC AGACCGTGAA
6661 GGTTGTGGAC GAGCTGGTGA AGGTCATGGG CAGGCACAAG CCTGAGAACA TCGTCATTGA
6721 GATGGCCCGG GAGAATCAGA CCACGCAGAA GGGCCAGAAG AACTCACGCG AGAGGATGAA
6781 GAGGATCGAG GAGGGCATTA AGGAGCTGGG GTCCCAGATC CTCAAGGAGC ACCCGGTGGA
6841 GAACACGCAG CTGCAGAATG AGAAGCTCTA CCTGTACTAC CTCCAGAATG GCCGCGATAT
6901 GTATGTGGAC CAGGAGCTGG ATATTAACAG GCTCAGCGAT TACGACGTCG ATCATATCGT
6961 TCCACAGTCA TTCCTGAAGG ATGACTCCAT TGACAACAAG GTCCTCACCA GGTCGGACAA
7021 GAACCGGGGC AAGTCTGATA ATGTTCCTTC AGAGGAGGTC GTTAAGAAGA TGAAGAACTA
7081 CTGGCGCCAG CTCCTGAATG CCAAGCTGAT CACGCAGCGG AAGTTCGATA ACCTCACAAA
7141 GGCTGAGAGG GGCGGGCTCT CTGAGCTGGA CAAGGCGGGC TTCATCAAGA GGCAGCTGGT
7201 CGAGACACGG CAGATCACTA AGCACGTTGC GCAGATTCTC GACTCACGGA TGAACACTAA
7261 GTACGATGAG AATGACAAGC TGATCCGCGA GGTGAAGGTC ATCACCCTGA AGTCAAAGCT
7321 CGTCTCCGAC TTCAGGAAGG ATTTCCAGTT CTACAAGGTT CGGGAGATCA ACAATTACCA
7381 CCATGCCCAT GACGCGTACC TGAACGCGGT GGTCGGCACA GCTCTGATCA AGAAGTACCC
7441 AAAGCTCGAG AGCGAGTTCG TGTACGGGGA CTACAAGGTT TACGATGTGA GGAAGATGAT
7501 CGCCAAGTCG GAGCAGGAGA TTGGCAAGGC TACCGCCAAG TACTTCTTCT ACTCTAACAT
7561 TATGAATTTC TTCAAGACAG AGATCACTCT GGCCAATGGC GAGATCCGGA AGCGCCCCCT
7621 CATCGAGACG AACGGCGAGA CGGGGGAGAT CGTGTGGGAC AAGGGCAGGG ATTTCGCGAC
7681 CGTCAGGAAG GTTCTCTCCA TGCCACAAGT GAATATCGTC AAGAAGACAG AGGTCCAGAC
7741 TGGCGGGTTC TCTAAGGAGT CAATTCTGCC TAAGCGGAAC AGCGACAAGC TCATCGCCCG
7801 CAAGAAGGAC TGGGATCCGA AGAAGTACGG CGGGTTCGAC AGCCCCACTG TGGCCTACTC
7861 GGTCCTGGTT GTGGCGAAGG TTGAGAAGGG CAAGTCCAAG AAGCTCAAGA GCGTGAAGGA
7921 GCTGCTGGGG ATCACGATTA TGGAGCGCTC CAGCTTCGAG AAGAACCCGA TCGATTTCCT
7981 GGAGGCGAAG GGCTACAAGG AGGTGAAGAA GGACCTGATC ATTAAGCTCC CCAAGTACTC
8041 ACTCTTCGAG CTGGAGAACG GCAGGAAGCG GATGCTGGCT TCCGCTGGCG AGCTGCAGAA
8101 GGGGAACGAG CTGGCTCTGC CGTCCAAGTA TGTGAACTTC CTCTACCTGG CCTCCCACTA
8161 CGAGAAGCTC AAGGGCAGCC CCGAGGACAA CGAGCAGAAG CAGCTGTTCG TCGAGCAGCA
8221 CAAGCATTAC CTCGACGAGA TCATTGAGCA GATTTCCGAG TTCTCCAAGC GCGTGATCCT
8281 GGCCGACGCG AATCTGGATA AGGTCCTCTC CGCGTACAAC AAGCACCGCG ACAAGCCAAT
8341 CAGGGAGCAG GCTGAGAATA TCATTCATCT CTTCACCCTG ACGAACCTCG GCGCCCCTGC
8401 TGCTTTCAAG TACTTCGACA CAACTATCGA TCGCAAGAGG TACACAAGCA CTAAGGAGGT
8461 CCTGGACGCG ACCCTCATCC ACCAGTCGAT TACCGGCCTC TACGAGACGC GCATCGACCT
8521 GTCTCAGCTC GGGGGCGACA AGCGGCCAGC GGCGACGAAG AAGGCGGGGC AGGCGAAGAA
8581 GAAGAAGTGA GCTCAGAGCT TTCGTTCGTA TCATCGGTTT CGACAACGTT CGTCAAGTTC
8641 AATGCATCAG TTTCATTGCG CACACACCAG AATCCTACTG AGTTTGAGTA TTATGGCATT
8701 GGGAAAACTG TTTTTCTTGT ACCATTTGTT GTGCTTGTAA TTTACTGTGT TTTTTATTCG
8761 GTTTTCGCTA TCGAACTGTG AAATGGAAAT GGATGGAGAA GAGTTAATGA ATGATATGGT
8821 CCTTTTGTTC ATTCTCAAAT TAATATTATT TGTTTTTTCT CTTATTTGTT GTGTGTTGAA
8881 TTTGAAATTA TAAGAGATAT GCAAACATTT TGTTTTGAGT AAAAATGTGT CAAATCGTGG
8941 CCTCTAATGA CCGAAGTTAA TATGAGGAGT AAAACACTTG TAGTTGTACC ATTATGCTTA
9001 TTCACTAGGC AACAAATATA TTTTCAGACC TAGAAAAGCT GCAAATGTTA CTGAATACAA
9061 GTATGTCCTC TTGTGTTTTA GACATTTATG AACTTTCCTT TATGTAATTT TCCAGAATCC
9121 TTGTCAGATT CTAATCATTG CTTTATAATT ATAGTTATAC TCATGGATTT GTAGTTGAGT
9181 ATGAAAATAT TTTTTAATGC ATTTTATGAC TTGCCAATTG ATTGACAACG AATTCGTAAT
9241 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC
9301 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA
9361 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT
9421 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG CTAGAGCAGC TTGCCAACAT
9481 GGTGGAGCAC GACACTCTCG TCTACTCCAA GAATATCAAA GATACAGTCT CAGAAGACCA
9541 AAGGGCTATT GAGACTTTTC AACAAAGGGT AATATCGGGA AACCTCCTCG GATTCCATTG
9601 CCCAGCTATC TGTCACTTCA TCAAAAGGAC AGTAGAAAAG GAAGGTGGCA CCTACAAATG
9661 CCATCATTGC GATAAAGGAA AGGCTATCGT TCAAGATGCC TCTGCCGACA GTGGTCCCAA
9721 AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC
9781 AAAGCAAGTG GATTGATGTG ATAACATGGT GGAGCACGAC ACTCTCGTCT ACTCCAAGAA
9841 TATCAAAGAT ACAGTCTCAG AAGACCAAAG GGCTATTGAG ACTTTTCAAC AAAGGGTAAT
9901 ATCGGGAAAC CTCCTCGGAT TCCATTGCCC AGCTATCTGT CACTTCATCA AAAGGACAGT
9961 AGAAAAGGAA GGTGGCACCT ACAAATGCCA TCATTGCGAT AAAGGAAAGG CTATCGTTCA
10021 AGATGCCTCT GCCGACAGTG GTCCCAAAGA TGGACCCCCA CCCACGAGGA GCATCGTGGA
10081 AAAAGAAGAC GTTCCAACCA CGTCTTCAAA GCAAGTGGAT TGATGTGATA TCTCCACTGA
10141 CGTAAGGGAT GACGCACAAT CCCACTATCC TTCGCAAGAC CTTCCTCTAT ATAAGGAAGT
10201 TCATTTCATT TGGAGAGGAC ACGCTGAAAT CACCAGTCTC TCTCTACAAA TCTATCTCTC
10261 TCGAGTCTAC CATGAGCCCA GAACGACGCC CGGCCGACAT CCGCCGTGCC ACCGAGGCGG
10321 ACATGCCGGC GGTCTGCACC ATCGTCAACC ACTACATCGA GACAAGCACG GTCAACTTCC
10381 GTACCGAGCC GCAGGAACCG CAGGAGTGGA CGGACGACCT CGTCCGTCTG CGGGAGCGCT
10441 ATCCCTGGCT CGTCGCCGAG GTGGACGGCG AGGTCGCCGG CATCGCCTAC GCGGGCCCCT
10501 GGAAGGCACG CAACGCCTAC GACTGGACGG CCGAGTCGAC CGTGTACGTC TCCCCCCGCC
10561 ACCAGCGGAC GGGACTGGGC TCCACGCTCT ACACCCACCT GCTGAAGTCC CTGGAGGCAC
10621 AGGGCTTCAA GAGCGTGGTC GCTGTCATCG GGCTGCCCAA CGACCCGAGC GTGCGCATGC
10681 ACGAGGCGCT CGGATATGCC CCCCGCGGCA TGCTGCGGGC GGCCGGCTTC AAGCACGGGA
10741 ACTGGCATGA CGTGGGTTTC TGGCAGCTGG ACTTCAGCCT GCCGGTACCG CCCCGTCCGG
10801 TCCTGCCCGT CACCGAGATT TGACTCGAGT TTCTCCATAA TAATGTGTGA GTAGTTCCCA
10861 GATAAGGGAA TTAGGGTTCC TATAGGGTTT CGCTCATGTG TTGAGCATAT AAGAAACCCT
10921 TAGTATGTAT TTGTATTTGT AAAATACTTC TATCAATAAA ATTTCTAATT CCTAAAACCA
10981 AAATCCAGTA CTAAAATCCA GATCCCCCGA ATTAATTCGG CGTTAATTCA GTACATTAAA
11041 AACGTCCGCA ATGTGTTATT AAGTTGTCTA AGCGTCAATT TGTTTACACC ACAATATATC
11101 CTGCCACCAG CCAGCCAACA GCTCCCCGAC CGGCAGCTCG GCACAAAATC ACCACTCGAT
11161 ACAGGCAGCC CATCAGTCCG GGACGGCGTC AGCGGGAGAG CCGTTGTAAG GCGGCAGACT
11221 TTGCTCATGT TACCGATGCT ATTCGGAAGA ACGGCAACTA AGCTGCCGGG TTTGAAACAC
11281 GGATGATCTC GCGGAGGGTA GCATGTTGAT TGTAACGATG ACAGAGCGTT GCTGCCTGTG
11341 ATCACCGCGG TTTCAAAATC GGCTCCGTCG ATACTATGTT ATACGCCAAC TTTGAAAACA
11401 ACTTTGAAAA AGCTGTTTTC TGGTATTTAA GGTTTTAGAA TGCAAGGAAC AGTGAATTGG
11461 AGTTCGTCTT GTTATAATTA GCTTCTTGGG GTATCTTTAA ATACTGTAGA AAAGAGGAAG
11521 GAAATAATAA ATGGCTAAAA TGAGAATATC ACCGGAATTG AAAAAACTGA TCGAAAAATA
11581 CCGCTGCGTA AAAGATACGG AAGGAATGTC TCCTGCTAAG GTATATAAGC TGGTGGGAGA
11641 AAATGAAAAC CTATATTTAA AAATGACGGA CAGCCGGTAT AAAGGGACCA CCTATGATGT
11701 GGAACGGGAA AAGGACATGA TGCTATGGCT GGAAGGAAAG CTGCCTGTTC CAAAGGTCCT
11761 GCACTTTGAA CGGCATGATG GCTGGAGCAA TCTGCTCATG AGTGAGGCCG ATGGCGTCCT
11821 TTGCTCGGAA GAGTATGAAG ATGAACAAAG CCCTGAAAAG ATTATCGAGC TGTATGCGGA
11881 GTGCATCAGG CTCTTTCACT CCATCGACAT ATCGGATTGT CCCTATACGA ATAGCTTAGA
11941 CAGCCGCTTA GCCGAATTGG ATTACTTACT GAATAACGAT CTGGCCGATG TGGATTGCGA
12001 AAACTGGGAA GAAGACACTC CATTTAAAGA TCCGCGCGAG CTGTATGATT TTTTAAAGAC
12061 GGAAAAGCCC GAAGAGGAAC TTGTCTTTTC CCACGGCGAC CTGGGAGACA GCAACATCTT
12121 TGTGAAAGAT GGCAAAGTAA GTGGCTTTAT TGATCTTGGG AGAAGCGGCA GGGCGGACAA
12181 GTGGTATGAC ATTGCCTTCT GCGTCCGGTC GATCAGGGAG GATATCGGGG AAGAACAGTA
12241 TGTCGAGCTA TTTTTTGACT TACTGGGGAT CAAGCCTGAT TGGGAGAAAA TAAAATATTA
12301 TATTTTACTG GATGAATTGT TTTAGTACCT AGAATGCATG ACCAAAATCC CTTAACGTGA
12361 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
12421 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
12481 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
12541 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
12601 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
12661 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
12721 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
12781 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
12841 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
12901 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
12961 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
13021 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC
13081 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG
13141 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT
13201 TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG
13261 CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG
13321 GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG
13381 GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA
13441 CCGTCATCAC CGAAACGCGC GAGGCAGGGT GCCTTGATGT GGGCGCCGGC GGTCGAGTGG
13501 CGACGGCGCG GCTTGTCCGC GCCCTGGTAG ATTGCCTGGC CGTAGGCCAG CCATTTTTGA
13561 GCGGCCAGCG GCCGCGATAG GCCGACGCGA AGCGGCGGGG CGTAGGGAGC GCAGCGACCG
13621 AAGGGTAGGC GCTTTTTGCA GCTCTTCGGC TGTGCGCTGG CCAGACAGTT ATGCACAGGC
13681 CAGGCGGGTT TTAAGAGTTT TAATAAGTTT TAAAGAGTTT TAGGCGGAAA AATCGCCTTT
13741 TTTCTCTTTT ATATCAGTCA CTTACATGTG TGACCGGTTC CCAATGTACG GCTTTGGGTT
13801 CCCAATGTAC GGGTTCCGGT TCCCAATGTA CGGCTTTGGG TTCCCAATGT ACGTGCTATC
13861 CACAGGAAAC AGACCTTTTC GACCTTTTTC CCCTGCTAGG GCAATTTGCC CTAGCATCTG
13921 CTCCGTACAT TAGGAACCGG CGGATGCTTC GCCCTCGATC AGGTTGCGGT AGCGCATGAC
13981 TAGGATCGGG CCAGCCTGCC CCGCCTCCTC CTTCAAATCG TACTCCGGCA GGTCATTTGA
14041 CCCGATCAGC TTGCGCACGG TGAAACAGAA CTTCTTGAAC TCTCCGGCGC TGCCACTGCG
14101 TTCGTAGATC GTCTTGAACA ACCATCTGGC TTCTGCCTTG CCTGCGGCGC GGCGTGCCAG
14161 GCGGTAGAGA AAACGGCCGA TGCCGGGATC GATCAAAAAG TAATCGGGGT GAACCGTCAG
14221 CACGTCCGGG TTCTTGCCTT CTGTGATCTC GCGGTACATC CAATCAGCTA GCTCGATCTC
14281 GATGTACTCC GGCCGCCCGG TTTCGCTCTT TACGATCTTG TAGCGGCTAA TCAAGGCTTC
14341 ACCCTCGGAT ACCGTCACCA GGCGGCCGTT CTTGGCCTTC TTCGTACGCT GCATGGCAAC
14401 GTGCGTGGTG TTTAACCGAA TGCAGGTTTC TACCAGGTCG TCTTTCTGCT TTCCGCCATC
14461 GGCTCGCCGG CAGAACTTGA GTACGTCCGC AACGTGTGGA CGGAACACGC GGCCGGGCTT
14521 GTCTCCCTTC CCTTCCCGGT ATCGGTTCAT GGATTCGGTT AGATGGGAAA CCGCCATCAG
14581 TACCAGGTCG TAATCCCACA CACTGGCCAT GCCGGCCGGC CCTGCGGAAA CCTCTACGTG
14641 CCCGTCTGGA AGCTCGTAGC GGATCACCTC GCCAGCTCGT CGGTCACGCT TCGACAGACG
14701 GAAAACGGCC ACGTCCATGA TGCTGCGACT ATCGCGGGTG CCCACGTCAT AGAGCATCGG
14761 AACGAAAAAA TCTGGTTGCT CGTCGCCCTT GGGCGGCTTC CTAATCGACG GCGCACCGGC
14821 TGCCGGCGGT TGCCGGGATT CTTTGCGGAT TCGATCAGCG GCCGCTTGCC ACGATTCACC
14881 GGGGCGTGCT TCTGCCTCGA TGCGTTGCCG CTGGGCGGCC TGCGCGGCCT TCAACTTCTC
14941 CACCAGGTCA TCACCCAGCG CCGCGCCGAT TTGTACCGGG CCGGATGGTT TGCGACCGCT
15001 CACGCCGATT CCTCGGGCTT GGGGGTTCCA GTGCCATTGC AGGGCCGGCA GACAACCCAG
15061 CCGCTTACGC CTGGCCAACC GCCCGTTCCT CCACACATGG GGCATTCCAC GGCGTCGGTG
15121 CCTGGTTGTT CTTGATTTTC CATGCCGCCT CCTTTAGCCG CTAAAATTCA TCTACTCATT
15181 TATTCATTTG CTCATTTACT CTGGTAGCTG CGCGATGTAT TCAGATAGCA GCTCGGTAAT
15241 GGTCTTGCCT TGGCGTACCG CGTACATCTT CAGCTTGGTG TGATCCTCCG CCGGCAACTG
15301 AAAGTTGACC CGCTTCATGG CTGGCGTGTC TGCCAGGCTG GCCAACGTTG CAGCCTTGCT
15361 GCTGCGTGCG CTCGGACGGC CGGCACTTAG CGTGTTTGTG CTTTTGCTCA TTTTCTCTTT
15421 ACCTCATTAA CTCAAATGAG TTTTGATTTA ATTTCAGCGG CCAGCGCCTG GACCTCGCGG
15481 GCAGCGTCGC CCTCGGGTTC TGATTCAAGA ACGGTTGTGC CGGCGGCGGC AGTGCCTGGG
15541 TAGCTCACGC GCTGCGTGAT ACGGGACTCA AGAATGGGCA GCTCGTACCC GGCCAGCGCC
15601 TCGGCAACCT CACCGCCGAT GCGCGTGCCT TTGATCGCCC GCGACACGAC AAAGGCCGCT
15661 TGTAGCCTTC CATCCGTGAC CTCAATGCGC TGCTTAACCA GCTCCACCAG GTCGGCGGTG
15721 GCCCATATGT CGTAAGGGCT TGGCTGCACC GGAATCAGCA CGAAGTCGGC TGCCTTGATC
15781 GCGGACACAG CCAAGTCCGC CGCCTGGGGC GCTCCGTCGA TCACTACGAA GTCGCGCCGG
15841 CCGATGGCCT TCACGTCGCG GTCAATCGTC GGGCGGTCGA TGCCGACAAC GGTTAGCGGT
15901 TGATCTTCCC GCACGGCCGC CCAATCGCGG GCACTGCCCT GGGGATCGGA ATCGACTAAC
15961 AGAACATCGG CCCCGGCGAG TTGCAGGGCG CGGGCTAGAT GGGTTGCGAT GGTCGTCTTG
16021 CCTGACCCGC CTTTCTGGTT AAGTACAGCG ATAACCTTCA TGCGTTCCCC TTGCGTATTT
16081 GTTTATTTAC TCATCGCATC ATATACGCAG CGACCGCATG ACGCAAGCTG TTTTACTCAA
16141 ATACACATCA CCTTTTTAGA CGGCGGCGCT CGGTTTCTTC AGCGGCCAAG CTGGCCGGCC
16201 AGGCCGCCAG CTTGGCATCA GACAAACCGG CCAGGATTTC ATGCAGCCGC ACGGTTGAGA
16261 CGTGCGCGGG CGGCTCGAAC ACGTACCCGG CCGCGATCAT CTCCGCCTCG ATCTCTTCGG
16321 TAATGAAAAA CGGTTCGTCC TGGCCGTCCT GGTGCGGTTT CATGCTTGTT CCTCTTGGCG
16381 TTCATTCTCG GCGGCCGCCA GGGCGTCGGC CTCGGTCAAT GCGTCCTCAC GGAAGGCACC
16441 GCGCCGCCTG GCCTCGGTGG GCGTCACTTC CTCGCTGCGC TCAAGTGCGC GGTACAGGGT
16501 CGAGCGATGC ACGCCAAGCA GTGCAGCCGC CTCTTTCACG GTGCGGCCTT CCTGGTCGAT
16561 CAGCTCGCGG GCGTGCGCGA TCTGTGCCGG GGTGAGGGTA GGGCGGGGGC CAAACTTCAC
16621 GCCTCGGGCC TTGGCGGCCT CGCGCCCGCT CCGGGTGCGG TCGATGATTA GGGAACGCTC
16681 GAACTCGGCA ATGCCGGCGA ACACGGTCAA CACCATGCGG CCGGCCGGCG TGGTGGTGTC
16741 GGCCCACGGC TCTGCCAGGC TACGCAGGCC CGCGCCGGCC TCCTGGATGC GCTCGGCAAT
16801 GTCCAGTAGG TCGCGGGTGC TGCGGGCCAG GCGGTCTAGC CTGGTCACTG TCACAACGTC
16861 GCCAGGGCGT AGGTGGTCAA GCATCCTGGC CAGCTCCGGG CGGTCGCGCC TGGTGCCGGT
16921 GATCTTCTCG GAAAACAGCT TGGTGCAGCC GGCCGCGTGC AGTTCGGCCC GTTGGTTGGT
16981 CAAGTCCTGG TCGTCGGTGC TGACGCGGGC ATAGCCCAGC AGGCCAGCGG CGGCGCTCTT
17041 GTTCATGGCG TAATGTCTCC GGTTCTAGTC GCAAGTATTC TACTTTATGC GACTAAAACA
17101 CGCGACAAGA AAACGCCAGG AAAAGGGCAG GGCGGCAGCC TGTCGCGTAA CTTAGGACTT
17161 GTGCGACATG TCGTTTTCAG AAGACGGCTG CACTGAACGT CAGAAGCCGA CTGCACTATA
17221 GCAGCGGAGG GGTTGGATCA AAGTACTTTG ATCCCGAGGG GAACCCTGTG GTTGGCATGC
17281 ACATACAAAT GGACGAACGG ATAAACCTTT TCACGCCCTT TTAAATATCC GTTATTCTAA
