测序及更多信息：https://pan.baidu.com/s/10BOkc3kZcJ_mMQGfxlBGJA 密码：gdjs
采购地址：淘宝店铺地址
pMy-BirA-T2A-eGFP
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载体名称:
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pMy-BirA-T2A-eGFP
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质粒类型:
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哺乳动物细胞逆病毒，表达载体,生物素连接酶BirA
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高拷贝/低拷贝:
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低拷贝
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启动子:
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CMV
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克隆方法:
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多克隆位点，限制性内切酶
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载体大小:
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载体标签:
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EGFP
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载体抗性:
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氨苄
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筛选标记:
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EFGP
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备注:
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逆病毒基因沉默载体。
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稳定性:
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稳表达
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组成型:
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组成型
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病毒/非病毒:
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逆病毒
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文献
A genome-wide map
of CTCF multivalency redefines the CTCF code. Nakahashi H, Kwon KR, Resch W, Vian L, Dose M,
Stavreva D, Hakim O, Pruett N, Nelson S, Yamane A, Qian J, Dubois W, Welsh S, Phair
RD, Pugh BF, Lobanenkov V, Hager GL, Casellas R. Cell Rep. 2013 May 30;3(5):1678-89. doi:
10.1016/j.celrep.2013.04.024. Epub 2013 May 23.10.1016/j.celrep.2013.04.024 PubMed 23707059
全序列
1 CTGCATAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG
61 CAAGGCATGG AAAAATACAT AACTGAGAAT AGAAAAGTTC AGATCAAGGT CAGGAACAGA
121 TGGAACAGCT GAATATGGGC CAAAGCGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC
181 AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG
241 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA
301 GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC
361 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG
421 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT
481 CGCCCGGGTA CCCGTATTCC CAATAAAGCC TCTTGCTGTT TGCATCCGAA TCGTGGACTC
541 GCTGATCCTT GGGAGGGTCT CCTCAGATTG ATTGACTGCC CACCTCGGGG GTCTTTCATT
601 TGGAGGTTCC ACCGAGATTT GGAGACCCCA GCCCAGGGAC CACCGACCCC CCCGCCGGGA
661 GGTAAGCTGG CCAGCGGTCG TTTCGTGTCT GTCTCTGTCT TTGTGCGTGT TTGTGCCGGC
721 ATCTAATGTT TGCGCCTGCG TCTGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC
781 CGTGGTGGAA CTGACGAGTT CGGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGTCG
841 GGGGCCGTTT TTGTGGCCCG ACCTGAGTCC AAAAAGCCCG ATCGTTTTGG ACTCTTTGGT
901 GCACCCCCCT TAGAGGAGGG ATATGTGGTT CTGGTAGGAG ACGAGAACCT AAAACAGTTC
961 CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGGACCGAA GCCGCGCCGC GCGTCTTGTC
1021 TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT CTGACTGTGT TTCTGTATTT GTCTGAAAAT
1081 ATGGGCCCGG GCCAGACTGT TACCACTCCC TTAAGTTTGA CCTTAGGTCA CTGGAAAGAT
1141 GTCGAGCGGA TCGCTCACAA CCAGTCGGTA GATGTCAAGA AGAGACGTTG GGTTACCTTC
1201 TGCTCTGCAG AATGGCCAAC CTTTAACGTC GGATGGCCGC GAGACGGCAC CTTTAACCGA
1261 GACCTCATCA CCCAGGTTAA GATCAAGGTC TTTTCACCTG GCCCGCATGG ACACCCAGAC
1321 CAGGTCCCCT ACATCGTGAC CTGGGAAGCC TTGGCTTTTG ACCCCCCTCC CTGGGTCAAG
1381 CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT CTTCCTCCAT CCGCCCCGTC TCTCCCCCTT
1441 GAACCTCCTC GTTCGACCCC GCCTCGATCC TCCCTTTATC CAGCCCTCAC TCCTTCTCTA
1501 GGCGCCCCCA TATGGCCATA TGAGATCTTA TATGGGGCAC CCCCGCCCCT TGTAAACTTC
1561 CCTGACCCTG ACATGACAAG AGTTACTAAC AGCCCCTCTC TCCAAGCTCA CTTACAGGCT
1621 CTCTACTTAG TCCAGCACGA AGTCTGGAGA CCTCTGGCGG CAGCCTACCA AGAACAACTG
1681 GACCGACCGG TGGTACCTCA CCCTTACCGA GTCGGCGACA CAGTGTGGGT CCGCCGACAC
1741 CAGACTAAGA ACCTAGAACC TCGCTGGAAA GGACCTTACA CAGTCCTGCT GACCACCCCC
1801 ACCGCCCTCA AAGTAGACGG CATCGCAGCT TGGATACACG CCGCCCACGT GAAGGCTGCC
1861 GACCCCGGGG GTGGACCATC CTCTAGACTG CCGGATCCGC CACCATGAAG GATAACACCG
1921 TGCCACTGAA ATTGATTGCC CTGTTAGCGA ACGGTGAATT TCACTCTGGC GAGCAGTTGG
1981 GTGAAACGCT GGGAATGAGC CGGGCGGCTA TTAATAAACA CATTCAGACA CTGCGTGACT
2041 GGGGCGTTGA TGTCTTTACC GTTCCGGGTA AAGGATACAG CCTGCCTGAG CCTATCCAGT
2101 TACTTAATGC TAAACAGATA TTGGGTCAGC TGGATGGCGG TAGTGTAGCC GTGCTGCCAG
2161 TGATTGACTC CACGAATCAG TACCTTCTTG ATCGTATCGG AGAGCTTAAA TCGGGCGATG
2221 CTTGCATTGC AGAATACCAG CAGGCTGGCC GTGGTCGCCG GGGTCGGAAA TGGTTTTCGC
2281 CTTTTGGCGC AAACTTATAT TTGTCGATGT TCTGGCGTCT GGAACAAGGC CCGGCGGCGG
2341 CGATTGGTTT AAGTCTGGTT ATCGGTATCG TGATGGCGGA AGTATTACGC AAGCTGGGTG
2401 CAGATAAAGT TCGTGTTAAA TGGCCTAATG ACCTCTATCT GCAGGATCGC AAGCTGGCAG
2461 GCATTCTGGT GGAGCTGACT GGCAAAACTG GCGATGCGGC GCAAATAGTC ATTGGAGCCG
2521 GGATCAACAT GGCAATGCGC CGTGTTGAAG AGAGTGTCGT TAATCAGGGG TGGATCACGC
2581 TGCAGGAAGC GGGGATCAAT CTCGATCGTA ATACGTTGGC GGCCATGCTA ATACGTGAAT
2641 TACGTGCTGC GTTGGAACTC TTCGAACAAG AAGGATTGGC ACCTTATCTG TCGCGCTGGG
2701 AAAAGCTGGA TAATTTTATT AATCGCCCAG TGAAACTTAT CATTGGTGAT AAAGAAATAT
2761 TTGGCATTTC ACGCGGAATA GACAAACAGG GGGCTTTATT ACTTGAGCAG GATGGAATAA
2821 TAAAACCCTG GATGGGCGGT GAAATATCCT TGCGTAGTGC AGAAAAAGAG GGCAGAGGAA
2881 GTCTTCTAAC ATGCGGTGAC GTGGAGGAGA ATCCCGGCCC TATGGTGAGC AAGGGCGAGG
2941 AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA
3001 AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT
3061 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT
3121 ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT
3181 CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT
3241 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA
3301 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA
3361 ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA
3421 AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA
3481 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG
3541 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG
3601 CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTA AACTCGACAT TTCGACCGGG
3661 CCGCCAGCAC AGTGGTCGAT CGAGGGGGGG CCCGCGATTA GTCCAATTTG TTAAAGACAG
3721 GATATCAGTG GTCCAGGCTC TAGTTTTGAC TCAACAATAT CACCAGCTGA AGCCTATAGA
3781 GTACGAGCCA TAGATAGAAT AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAATGAA
3841 AGACCCCACC TGTAGGTTTG GCAAGCTAGC TTAAGTAACG CCATTTTGCA AGGCATGGAA
3901 AATACATAAC TGAGAATAGA GAAGTTCAGA TCAAGGTTAG GAACAGAGAG ACAGCAGAAT
3961 ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG
4021 TTGGAACAGC AGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
4081 CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCCGCCC TCAGCAGTTT CTAGAGAACC
4141 ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA
4201 CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA
4261 GCCCACAACC CCTCACTCGG CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG
4321 TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG
4381 GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT TTCACATGCA GCATGTATCA
4441 AAATTAATTT GGTTTTTTTT CTTAAGTATT TACATTAAAT GGCCATAGTT GCATTAATGA
4501 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC
4561 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
4621 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC
4681 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
4741 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
4801 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
4861 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
4921 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
4981 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
5041 AACCCGGTAA GACACGACTT ATAGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
5101 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
5161 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
5221 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
5281 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
5341 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
5401 AGGATCTTCA CCTAGATCCT TTTGCGGCCG GCCGCAAATC AATCTAAAGT ATATATGAGT
5461 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
5521 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
5581 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
5641 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT
5701 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
5761 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
5821 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA
5881 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
5941 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT
6001 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA
6061 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAACACGGGA TAATACCGCG CCACATAGCA
6121 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
6181 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
6241 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA
6301 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT
6361 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
6421 ATAAACAAAT AGGGGTTCCG CGCACATTTC
