• PURPOSE

  expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

BACKBONE

• Vector backbone
  T7-VEE-IRES-puro (Addgene plasmid 58970)
• Backbone manufacturer
  Dowdy lab
• Vector type
  Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon
• Selectable markers
  Puromycin

参考文献

Efficient generation of human iPSCs by a synthetic self-replicative RNA. Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001. 10.1016/j.stem.2013.06.001 PubMed 23910086