BACKBONE

• Vector backbone
  pEGFP-C1
• Backbone manufacturer
  Clontech
• Backbone size w/o insert (bp)4700
• Vector type
  Mammalian Expression
• Selectable markers
  Neomycin (select with G418)

GENE/INSERT

• Gene/Insert name
  Lactadherin-C2 Domain
• Species
  B. taurus (bovine)
• Insert Size (bp)
  475
• Entrez Gene
  MFGE8
• Tag / Fusion Protein
  • GFP (N terminal on backbone)

参考文献

Membrane phosphatidylserine regulates surface charge and protein localization. Yeung T, Gilbert GE, Shi J, Silvius J, Kapus A, Grinstein S. Science. 2008 Jan 11. 319(5860):210-3. 10.1126/science.1152066 PubMed 18187657