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慢病毒相关质粒

pMD2.G pMD2G 慢病毒包装质粒 (搭配质粒pSPAX2)
发布时间:2018-05-18 22:32:02 | 浏览次数:


测序及更多信息: https://pan.baidu.com/s/1Y8L1PLhPGl3S96R4ZyT1FQ?pwd=hedg

采购地址:淘宝店铺地址





质粒类型:        慢病毒载体包装载体

搭配质粒:pSPAX2

高拷贝/低拷贝:      高拷贝

启动子:   CMV

克隆方法:        多克隆位点,限制性内切酶

载体大小:        5824 bp

5' 测序引物及序列:       CMV Fwd: CGCAAATGGGCGGTAGGCGTG

载体抗性:        Ampicillin (氨苄青霉素)

备注:        Envelope plasmid. Known to work with most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system


全序列

1        GGATCCCCTG AGGGGGCCCC CATGGGCTAG AGGATCCGGC CTCGGCCTCT GCATAAATAA
61       AAAAAATTAG TCAGCCATGA GCTTGGCCCA TTGCATACGT TGTATCCATA TCATAATATG
121      TACATTTATA TTGGCTCATG TCCAACATTA CCGCCATGTT GACATTGATT ATTGACTAGT
181      TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT
241      ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG
301      TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG
361      GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT
421      ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG
481      ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG
541      GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT
601      CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC
661      TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG
721      TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT
781      CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCC CTCGAAGCTT
841      ACATGTGGTA CCGAGCTCGG ATCCTGAGAA CTTCAGGGTG AGTCTATGGG ACCCTTGATG
901      TTTTCTTTCC CCTTCTTTTC TATGGTTAAG TTCATGTCAT AGGAAGGGGA GAAGTAACAG
961      GGTACACATA TTGACCAAAT CAGGGTAATT TTGCATTTGT AATTTTAAAA AATGCTTTCT
1021     TCTTTTAATA TACTTTTTTG TTTATCTTAT TTCTAATACT TTCCCTAATC TCTTTCTTTC
1081     AGGGCAATAA TGATACAATG TATCATGCCT CTTTGCACCA TTCTAAAGAA TAACAGTGAT
1141     AATTTCTGGG TTAAGGCAAT AGCAATATTT CTGCATATAA ATATTTCTGC ATATAAATTG
1201     TAACTGATGT AAGAGGTTTC ATATTGCTAA TAGCAGCTAC AATCCAGCTA CCATTCTGCT
1261     TTTATTTTAT GGTTGGGATA AGGCTGGATT ATTCTGAGTC CAAGCTAGGC CCTTTTGCTA
1321     ATCATGTTCA TACCTCTTAT CTTCCTCCCA CAGCTCCTGG GCAACGTGCT GGTCTGTGTG
1381     CTGGCCCATC ACTTTGGCAA AGCACGTGAG ATCTGAATTC TGACACTATG AAGTGCCTTT
1441     TGTACTTAGC CTTTTTATTC ATTGGGGTGA ATTGCAAGTT CACCATAGTT TTTCCACACA
1501     ACCAAAAAGG AAACTGGAAA AATGTTCCTT CTAATTACCA TTATTGCCCG TCAAGCTCAG
1561     ATTTAAATTG GCATAATGAC TTAATAGGCA CAGCCATACA AGTCAAAATG CCCAAGAGTC
1621     ACAAGGCTAT TCAAGCAGAC GGTTGGATGT GTCATGCTTC CAAATGGGTC ACTACTTGTG
1681     ATTTCCGCTG GTATGGACCG AAGTATATAA CACAGTCCAT CCGATCCTTC ACTCCATCTG
1741     TAGAACAATG CAAGGAAAGC ATTGAACAAA CGAAACAAGG AACTTGGCTG AATCCAGGCT
1801     TCCCTCCTCA AAGTTGTGGA TATGCAACTG TGACGGATGC CGAAGCAGTG ATTGTCCAGG
1861     TGACTCCTCA CCATGTGCTG GTTGATGAAT ACACAGGAGA ATGGGTTGAT TCACAGTTCA
1921     TCAACGGAAA ATGCAGCAAT TACATATGCC CCACTGTCCA TAACTCTACA ACCTGGCATT
1981     CTGACTATAA GGTCAAAGGG CTATGTGATT CTAACCTCAT TTCCATGGAC ATCACCTTCT
2041     TCTCAGAGGA CGGAGAGCTA TCATCCCTGG GAAAGGAGGG CACAGGGTTC AGAAGTAACT
2101     ACTTTGCTTA TGAAACTGGA GGCAAGGCCT GCAAAATGCA ATACTGCAAG CATTGGGGAG
2161     TCAGACTCCC ATCAGGTGTC TGGTTCGAGA TGGCTGATAA GGATCTCTTT GCTGCAGCCA
2221     GATTCCCTGA ATGCCCAGAA GGGTCAAGTA TCTCTGCTCC ATCTCAGACC TCAGTGGATG
2281     TAAGTCTAAT TCAGGACGTT GAGAGGATCT TGGATTATTC CCTCTGCCAA GAAACCTGGA
2341     GCAAAATCAG AGCGGGTCTT CCAATCTCTC CAGTGGATCT CAGCTATCTT GCTCCTAAAA
2401     ACCCAGGAAC CGGTCCTGCT TTCACCATAA TCAATGGTAC CCTAAAATAC TTTGAGACCA
2461     GATACATCAG AGTCGATATT GCTGCTCCAA TCCTCTCAAG AATGGTCGGA ATGATCAGTG
2521     GAACTACCAC AGAAAGGGAA CTGTGGGATG ACTGGGCACC ATATGAAGAC GTGGAAATTG
2581     GACCCAATGG AGTTCTGAGG ACCAGTTCAG GATATAAGTT TCCTTTATAC ATGATTGGAC
2641     ATGGTATGTT GGACTCCGAT CTTCATCTTA GCTCAAAGGC TCAGGTGTTC GAACATCCTC
2701     ACATTCAAGA CGCTGCTTCG CAACTTCCTG ATGATGAGAG TTTATTTTTT GGTGATACTG
2761     GGCTATCCAA AAATCCAATC GAGCTTGTAG AAGGTTGGTT CAGTAGTTGG AAAAGCTCTA
2821     TTGCCTCTTT TTTCTTTATC ATAGGGTTAA TCATTGGACT ATTCTTGGTT CTCCGAGTTG
2881     GTATCCATCT TTGCATTAAA TTAAAGCACA CCAAGAAAAG ACAGATTTAT ACAGACATAG
2941     AGATGAACCG ACTTGGAAAG TAACTCAAAT CCTGCACAAC AGATTCTTCA TGTTTGGACC
3001     AAATCAACTT GTGATACCAT GCTCAAAGAG GCCTCAATTA TATTTGAGTT TTTAATTTTT
3061     ATGGAATTCA CCCCACCAGT GCAGGCTGCC TATCAGAAAG TGGTGGCTGG TGTGGCTAAT
3121     GCCCTGGCCC ACAAGTTTCA CTAAGCTCGC TTCCTTGCTG TCCAATTTCT ATTAAAGGTT
3181     CCTTGGTTCC CTAAGTCCAA CTACTAAACT GGGGGATATT ATGAAGGGCC TTGAGCATCT
3241     GGATTCTGCC TAATAAAAAA CATTTATTTT CATTGCAATG ATGTATTTAA ATTATTTCTG
3301     AATATTTTAC TAAAAAGGGA ATGTGGGAGG TCAGTGCATT TAAAACATAA AGAAATGAAG
3361     AGCTAGTTCA AACCTTGGGA AAATACACTA TATCTTAAAC TCCATGAAAG AAGGTGAGGC
3421     TGCAAACAGC TAATGCACAT TGGCAACAGC CCTGATGCCT ATGCCTTATT CATCCCTCAG
3481     AAAAGGATTC AAGTAGAGGC TTGATTTGGA GGTTAAAGTT TGGCTATGCT GTATTTTACA
3541     TTACTTATTG TTTTAGCTGT CCTCATGAAT GTCTTTTCAC TACCCATTTG CTTATCCTGC
3601     ATCTCTCAGC CTTGACTCCA CTCAGTTCTC TTGCTTAGAG ATACCACCTT TCCCCTGAAG
3661     TGTTCCTTCC ATGTTTTACG GCGAGATGGT TTCTCCTCGC CTGGCCACTC AGCCTTAGTT
3721     GTCTCTGTTG TCTTATAGAG GTCTACTTGA AGAAGGAAAA ACAGGGGGCA TGGTTTGACT
3781     GTCCTGTGAG CCCTTCTTCC CTGCCTCCCC CACTCACAGT GACCCGGAAT CCCTCGACAT
3841     GGCAGTCTAG CACTAGTGCG GCCGCAGATC TGCTTCCTCG CTCACTGACT CGCTGCGCTC
3901     GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
3961     AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
4021     CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
4081     CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
4141     GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
4201     CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA
4261     TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
4321     GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
4381     CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
4441     TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
4501     TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
4561     CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
4621     AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
4681     CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
4741     CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
4801     TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
4861     ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
4921     TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC
4981     AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
5041     CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
5101     GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
5161     TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
5221     AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
5281     ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
5341     CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
5401     GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
5461     AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
5521     GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
5581     CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
5641     GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
5701     TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
5761     AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GT






 
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