shRNA质粒

pSilencer 2.1-U6 hygro RNAi系统载体 shRNA

发布时间:2018-07-31 12:20:15 | 浏览次数：

测序及更多信息：https://pan.baidu.com/s/1t74JrJqXuPd5LD_x72W9Lg
提取码：8zq7

pSilencer 2.1-U6 hygro

pSilencer2.1-U6-hygro载体基本信息

载体名称:	pSilencer 2.1-U6 hygro
质粒类型:	RNAi系统载体
高拷贝/低拷贝:	高
启动子:	Human U6
载体大小:	4886bp
5' 测序引物及序列:	Human-U6： GGGCAGGAAGAGGGCCTA,
3' 测序引物及序列:	PMAL-C2X-R ：CTGCAAGGCGATTAAGTTGG
载体抗性:	Ampicillin
筛选标记:	hygro
稳定性:	瞬表达 Transient
组成型:	组成型 Constitutive
病毒/非病毒:	非病毒

全序列

1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTTTTCCAA AAAAACGTGA
421 GAGTGTCTAA CGGTCTCTTG AACCGTTAGA CACTCTCACG TGGATCCCGC GTCCTTTCCA
481 CAAGATATAT AAACCCAAGA AATCGAAATA CTTTCAAGTT ACGGTAAGCA TATGATAGTC
541 CATTTTAAAA CATAATTTTA AAACTGCAAA CTACCCAAGA AATTATTACT TTCTACGTCA
601 CGTATTTTGT ACTAATATCT TTGTGTTTAC AGTCAAATTA ATTCTAATTA TCTCTCTAAC
661 AGCCTTGTAT CGTATATGCA AATATGAAGG AATCATGGGA AATAGGCCCT CTTCCTGCCC
721 GACCTTGGCG CGCGCTCGGC GCGCGGTCAC GCTCCGTCAC GTGGTGCGTT TTGCCTGCGC
781 GTCTTTCCAC TGGGGAATTC ATGCTTCTCC TCCCTTTAGT GAGGGTAATT CTCTCTCTCT
841 CCCTATAGTG AGTCGTATTA ATTCCTTCTC TTCTATAGTG TCACCTAAAT CGTTGCAATT
901 CGTAATCATG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA
961 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC
1021 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA
1081 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC
1141 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC
1201 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
1261 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
1321 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
1381 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
1441 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
1501 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
1561 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
1621 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
1681 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
1741 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
1801 AAGAGTTGGT AGCTCTTGAT CCGGCAAAAA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
1861 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
1921 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
1981 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
2041 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
2101 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
2161 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG
2221 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
2281 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
2341 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
2401 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
2461 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
2521 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
2581 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
2641 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
2701 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
2761 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
2821 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
2881 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
2941 TTTTCAATAT TCAGGCTTAC CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC
3001 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT CTAGCTAGAG
3061 GTCGACGGTA TACAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA
3121 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA
3181 TTATAAGCTG CAATAAACAA GTTGGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC
3241 TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG
3301 GCGGCGGTGG AATCGAAATC TCGTAGCACG TGCTATTCCT TTGCCCTCGG ACGAGTGCTG
3361 GGGCGTCGGT TTCCACTATC GGCGAGTACT TCTACACAGC CATCGGTCCA GACGGCCGCG
3421 CTTCTGCGGG CGATTTGTGT ACGCCCGACA GTCCCGGCTC CGGATCGGAC GATTGCGTCG
3481 CATCGACCCT GCGCCCAAGC TGCATCATCG AAATTGCCGT CAACCAAGCT CTGATAGAGT
3541 TGGTCAAGAC CAATGCGGAG CATATACGCC CGGAGCCGCG GCGATCCTGC AAGCTCCGGA
3601 TGCCTCCGCT CGAAGTAGCG CGTCTGCTGC TCCATACAAG CCAACCACGG CCTCCAGAAG
3661 AAGATGTTGG CGACCTCGTA TTGGGAATCC CCGAACATCG CCTCGCTCCA GTCAATGACC
3721 GCTGTTATGC GGCCATTGTC CGTCAGGACA TTGTTGGAGC CGAAATCCGC GTGCACGAGG
3781 TGCCGGACTT CGGGGCAGTC CTCGGCCCAA AGCATCAGCT CATCGAGAGC CTGCGCGACG
3841 GACGCACTGA CGGTGTCGTC CATCACAGTT TGCCAGTGAT ACACATGGGG ATCAGCAATC
3901 GCGCATATGA AATCACGCCA TGTAGTGTAT TGACCGATTC CTTGCGGTCC GAATGGGCCG
3961 AACCCGCTCG TCTGGCTAAG ATCGGCCGCA GCGATCGCAT CCATGGCCTC CGCGACCGGC
4021 TGCAGAACAG CGGGCAGTTC GGTTTCAGGC AGGTCTTGCA ACGTGACACC CTGTGCACGG
4081 CGGGAGATGC AATAGGTCAG GCTCTCGCTG AATTCCCCAA TGTCAAGCAC TTCCGGAATC
4141 GGGAGCGCGG CCGATGCAAA GTGCCGATAA ACATAACGAT CTTTGTAGAA ACCATCGGCG
4201 CAGCTATTTA CCCGCAGGAC ATATCCACGC CCTCCTACAT CGAAGCTGAA AGCACGAGAT
4261 TCTTCGCCCT CCGAGAGCTG CATCAGGTCG GAGACGCTGT CGAACTTTTC GATCAGAAAC
4321 TTCTCGACAG ACGTCGCGGT GAGTTCAGGC TTTTTCATCA CGTGCTGATC AGATCCGAAA
4381 ATGGATATAC AAGCTCCCGG GAGCTTTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCC
4441 CACTACTTCT GGAATAGCTC AGAGGCAGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA
4501 ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC
4561 GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG
4621 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTA ACTAATTGAG ATGCATGCTT
4681 TGCATACTTC TGCCTGCTGG AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
4741 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC
4801 CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA
4861 TAGGCGTATC ACGAGGCCCT TTCGTC