测序及更多信息：https://pan.baidu.com/s/1_7-3TUn91by9k6D94Y4pbw 
提取码：xd4d 

采购地址：淘宝店铺地址

Super PiggyBac Transposase (PB200PA-1)载体简介：  The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors andchromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposonspecific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the piggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes.The Super PiggyBac transposase expression vector features a 5’ HS4 insulator to support robust transcription from the rPolr2A promoter. The PiggyBac transposase coding sequence has been optimized for high expression, stability and activity in mammalian cells.   Super PiggyBac Transposase (PB200PA-1)

全序列

1        GCGGCCGCGA GCTCACGGGG ACAGCCCCCC CCCAAAGCCC CCAGGGATGT AATTACGTCC
61       CTCCCCCGCT AGGGGGCAGC AGCGAGCCGC CCGGGGCTCC GCTCCGGTCC GGCGCTCCCC
121      CCGCATCCCC GAGCCGGCAG CGTGCGGGGA CAGCCCGGGC ACGGGGAAGG TGGCACGGGA
181      TCGCTTTCCT CTGAACGCTT CTCGCTGCTC TTTGAGCCTG CAGACACCTG GGGGGATACG
241      GGGAAAAAGC TTTAGGCTGA AAGAGAGATT TAGAATGACA GAATCATAGA ACGGCCTGGG
301      TTGCAAAGGA GCACAGTGCT CATCCAGATC CAACCCCCTG CTATGTGCAG GGTCATCAAC
361      CAGCAGCCCA GGCTGCCCAG AGCCACATCC AGCCTGGCCT TGAATGCCTG CAGGGATGGG
421      GCATCCACAG CCTCCTTGGG CAACCTGTTC AGTGCGTCAC CACCCTCTGG GGGAAAAACT
481      GCCTCCTCAT ATCCAACCCA AACCTCCCCT GTCTCAGTGT AAAGCCATTC CCCCTTGTCC
541      TATCAAGGGG GAGTTTGCTG TGACATTGTT GGTCTGGGGT GACACATGTT TGCCAATTCA
601      GTGCATCACG GAGAGGCAGA TCTTGGGGAT AAGGAAGTGC AGGACAGCAT GGACGTGGGA
661      CATGCAGGTG TTGAGGGCTC TGGGACACTC TCCAAGTCAC AGCGTTCAGA ACAGCCTTAA
721      GGATAAGAAG ATAGGATAGA AGGACAAAGA GCAAGTTAAA ACCCAGCATG GAGAGGAGCA
781      CAAAAAGGCC ACAGACACTG CTGGTCCCTG TGTCTGAGCC TGCATGTTTG ATGGTGTCTG
841      GATGCAAGCA GAAGGGGTGG AAGAGCTTGC CTGGAGAGAT ACAGCTGGGT CAGTAGGACT
901      GGGACAGGCA GCTGGAGAAT TGCCATGTAG ATGTTCATAC AATCGTCAAA TCATGAAGGC
961      TGGAAAAGCC CTCCAAGATC CCCAAGACCA ACCCCAACCC ACCCACCGTG CCCACTGGCC
1021     ATGTCCCTCA GTGCCACATC CCCACAGTTC TTCATCACCT CCAGGGACGG TGACCCCCCC
1081     ACCTCCGTGG GCAGCTGTGC CACTGCAGCA CCGCTCTTTG GAGAAGGTAA ATCTTGCTAA
1141     ATCCAGCCCG ACCCTCCCCT GGCACAACGT AAGGCCATTA TCTCTCATCC AACTCCAGGA
1201     CGGAGTCAGT GAGAATATTG CCTCCCTGGC GAGCTCACGG GGACAGCCCC CCCCCAAAGC
1261     CCCCAGGGAT GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT
1321     CCGCTCCGGT CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG
1381     GCACGGGGAA GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC
1441     TGCAGACACC TGGGGGGATA CGGGGAAAAA GCTTTAGGCT GAAAGAGAGA TTTAGAATGA
1501     CAGAATCATA GAACGGCCTG GGTTGCAAAG GAGCACAGTG CTCATCCAGA TCCAACCCCC
1561     TGCTATGTGC AGGGTCATCA ACCAGCAGCC CAGGCTGCCC AGAGCCACAT CCAGCCTGGC
1621     CTTGAATGCC TGCAGGGATG GGGCATCCAC AGCCTCCTTG GGCAACCTGT TCAGTGCGTC
1681     ACCACCCTCT GGGGGAAAAA CTGCCTCCTC ATATCCAACC CAAACCTCCC CTGTCTCAGT
1741     GTAAAGCCAT TCCCCCTTGT CCTATCAAGG GGGAGTTTGC TGTGACATTG TTGGTCTGGG
1801     GTGACACATG TTTGCCAATT CAGTGCATCA CGGAGAGGCA GATCTTGGGG ATAAGGAAGT
1861     GCAGGACAGC ATGGACGTGG GACATGCAGG TGTTGAGGGC TCTGGGACAC TCTCCAAGTC
1921     ACAGCGTTCA GAACAGCCTT AAGGATAAGA AGATAGGATA GAAGGACAAA GAGCAAGTTA
1981     AAACCCAGCA TGGAGAGGAG CACAAAAAGG CCACAGACAC TGCTGGTCCC TGTGTCTGAG
2041     CCTGCATGTT TGATGGTGTC TGGATGCAAG CAGAAGGGGT GGAAGAGCTT GCCTGGAGAG
2101     ATACAGCTGG GTCAGTAGGA CTGGGACAGG CAGCTGGAGA ATTGCCATGT AGATGTTCAT
2161     ACAATCGTCA AATCATGAAG GCTGGAAAAG CCCTCCAAGA TCCCCAAGAC CAACCCCAAC
2221     CCACCCACCG TGCCCACTGG CCATGTCCCT CAGTGCCACA TCCCCACAGT TCTTCATCAC
2281     CTCCAGGGAC GGTGACCCCC CCACCTCCGT GGGCAGCTGT GCCACTGCAG CACCGCTCTT
2341     TGGAGAAGGT AAATCTTGCT AAATCCAGCC CGACCCTCCC CTGGCACAAC GTAAGGCCAT
2401     TATCTCTCAT CCAACTCCAG GACGGAGTCA GTGAGAATAT TCGGCCGCTC TAGAACTAGT
2461     GGATCCCCCG GGCTGCAGGA ATTCGATGGC CGCTTGGGCG GGATATCTAG CGCCAAGGTT
2521     CCGTAAAGCT CTCTATTAGA GGACTGGCGG TGCCCCATCA CCCTGGGAGG CCTCTCCACA
2581     AATATCGGCT ACTTCCAATT GATTGGACGC GCCATCTTGT CTGCTTATGC ATATTCAGAG
2641     GATCCTGAAT ATTCATGAGC GAGGGCCGTG CGGCCCCTCC CTCCAACCCT CCCCCGGAAC
2701     AAGCTCCGGA GAACCCGACA GGCCCCGCCT TCTTTACCGA TGCGTAGAAC AAACCATTTT
2761     CCGGGTTGGG GGGGGAAATT AATGAGAGAC TTTAGCTGAA AATGAGCATG GACGCCAAAG
2821     CTGAGTAAAG ATGGCTTAAC TTTATCCTCC ATTCTGTAAT CCGTCAGCTT GAGTGTACGG
2881     GAAGTCGGCG AGGGGGGCGG CAGGCTCAGA AACATTCTCC TCCTCCTGTC GCGTCAGAAA
2941     GAACACCCAA CCAGGGAGCC GGAGCCCTGG CGTCAACATC TCTGGCGCGC GCGCTCCATG
3001     TAGGCCGGTG CGGGCGGCCC CGTAGCGCAA GGGAGGGCGG GAAAGGAAGG GGCGGGACAC
3061     AAGGGCGAAT CTATAAAGGG CGTCATTCAG CCAGTTCTCT CCTCAGAAGC GCCGAGAGCG
3121     CGACCGGGAC GGTTGGAGAA GAAGGTGGCT CCCGGAAGGG GGAGAGACAA ACTGCCGTAA
3181     CCTCTGCCGT TCAGGATCCC GGCCATCAAG CTTATCGATG CTGCCGCAGC AAAAGCAGGA
3241     GCAGATGCCG CCGTCGCAGG CGAAGATGTC GCAGACGGAG GAGGCGATGC TGCCGGCGGA
3301     GGAGGCGAAG TAAGTAGAGG GCTGGGCTGG GCTGTGGGGG GTGTGGGGTG CGGGACTGGG
3361     CAGTCTGGGA GTCCCTCTCA CCACTTTTCT TACCTTTCTA GGATGCTGCC TCGAGGAACT
3421     GAAAAACCAG AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA
3481     TCCGGTGGTG GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC
3541     CTGTACGGAA GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCAAT TCGTTAAGGC
3601     CAAATTGGCC ACCATGGGCT CTAGCCTGGA CGACGAGCAC ATCCTGAGCG CCCTGCTGCA
3661     GAGCGACGAC GAACTGGTGG GCGAGGACAG CGACAGCGAG GTCAGCGACC ACGTGTCCGA
3721     GGACGACGTG CAGTCCGACA CCGAGGAAGC CTTCATCGAC GAGGTGCACG AAGTGCAGCC
3781     TACCAGCAGC GGCTCCGAGA TCCTGGACGA GCAGAACGTG ATCGAGCAGC CTGGCAGCTC
3841     CCTGGCCAGC AACAGAATCC TGACCCTGCC CCAGAGAACC ATCAGAGGCA AGAACAAGCA
3901     CTGCTGGTCC ACCTCCAAGA GCACCAGGCG GAGCAGAGTG TCCGCCCTGA ACATCGTGCG
3961     GAGCCAGAGG GGCCCCACCA GAATGTGCAG AAACATCTAC GACCCCCTGC TGTGCTTCAA
4021     GCTGTTCTTC ACCGACGAGA TCATCAGCGA GATCGTGAAG TGGACCAACG CCGAGATCAG
4081     CCTGAAGAGG CGGGAGAGCA TGACCAGCGC CACCTTCAGA GACACCAACG AGGACGAGAT
4141     CTACGCCTTC TTCGGCATCC TGGTGATGAC CGCCGTGAGA AAGGACAACC ACATGAGCAC
4201     CGACGACCTG TTCGACAGAT CCCTGAGCAT GGTGTACGTG TCCGTGATGA GCAGAGACAG
4261     ATTCGACTTC CTGATCAGAT GCCTGAGAAT GGACGACAAG AGCATCAGAC CCACCCTGCG
4321     GGAGAACGAC GTGTTCACCC CCGTGCGGAA GATCTGGGAC CTGTTCATCC ACCAGTGCAT
4381     CCAGAACTAC ACCCCTGGCG CCCACCTGAC CATCGATGAG CAGCTGCTGG GCTTCAGAGG
4441     CAGATGCCCC TTCAGAGTGT ACATCCCCAA CAAGCCCAGC AAGTACGGCA TCAAGATCCT
4501     GATGATGTGC GACAGCGGCA CCAAGTACAT GATCAACGGC ATGCCCTACC TGGGCAGAGG
4561     CACCCAGACA AACGGCGTGC CCCTGGGCGA GTACTACGTG AAAGAACTGA GCAAGCCTGT
4621     GCATGGCAGC TGCAGGAACA TCACCTGCGA CAACTGGTTC ACCAGCATCC CCCTGGCCAA
4681     GAACCTGCTG CAGGAACCCT ACAAGCTGAC CATCGTGGGC ACCGTGCGGA GCAACAAGCG
4741     GGAGATCCCA GAGGTGCTGA AGAACAGCAG ATCCAGACCT GTGGGAACAA GCATGTTCTG
4801     CTTCGACGGC CCCCTGACCC TGGTGTCCTA CAAGCCCAAG CCCGCCAAGA TGGTGTACCT
4861     GCTGTCCAGC TGCGACGAGG ACGCCAGCAT CAACGAGAGC ACCGGCAAGC CCCAGATGGT
4921     GATGTACTAC AACCAGACCA AGGGCGGCGT GGACACCCTG GACCAGATGT GCAGCGTGAT
4981     GACCTGCAGC AGAAAGACCA ACAGATGGCC CATGGCCCTG CTGTACGGCA TGATCAATAT
5041     CGCCTGCATC AACAGCTTCA TCATCTACAG CCACAACGTG TCCAGCAAGG GCGAGAAGGT
5101     GCAGAGCCGG AAGAAATTCA TGCGGAACCT GTACATGAGC CTGACCTCCA GCTTCATGAG
5161     AAAGAGACTG GAAGCCCCCA CCCTGAAGAG ATACCTGCGG GACAACATCA GCAACATCCT
5221     GCCCAAGGAA GTGCCAGGAA CAAGCGACGA CAGCACCGAG GAACCCGTGA TGAAGAAGAG
5281     GACCTACTGC ACCTACTGTC CCAGCAAGAT CAGAAGAAAG GCCAACGCCA GCTGCAAGAA
5341     ATGCAAAAAA GTGATCTGCC GGGAGCACAA CATCGACATG TGCCAGAGCT GTTTCTGAGG
5401     CCGTAACGGC CGCCAGAATT GGGGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC
5461     AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG
5521     CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT
5581     TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTC GGATCCTCTA GAGTCGACCT
5641     GCAGGCATGC AAGCTCGGTA CCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT
5701     TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC
5761     ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC
5821     TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC
5881     TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
5941     CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
6001     ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
6061     GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
6121     ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
6181     ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
6241     CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
6301     CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
6361     TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
6421     GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
6481     GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
6541     ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
6601     GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
6661     TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
6721     TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
6781     GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
6841     TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
6901     CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
6961     TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC
7021     CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
7081     GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
7141     GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
7201     TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
7261     GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
7321     TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
7381     CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
7441     CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
7501     ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
7561     GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
7621     CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
7681     GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
7741     TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
7801     TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
7861     CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG
7921     TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC
7981     TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC
8041     GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA
8101     GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA
8161     ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG
8221     ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA
8281     AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCATTCGC CATTCAGGCT
8341     GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA
8401     AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG
8461     TTGTAAAACG ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT
8521     CCACCGCGGT G