测序及更多信息： 链接：https://pan.baidu.com/s/1fq1bjWeTuVxllGZOMKskqA
提取码：x3uv
采购地址：淘宝店铺地址
载体基本信息
|
出品公司:
|
HedgehogBio
|
|
载体名称:
|
pCDNA3.1(+)-h-STAT3
|
|
质粒类型:
|
人源STAT3(NM_139276.2)表达载体
|
|
启动子:
|
CMV
|
|
表达水平:
|
高
|
|
克隆方法:
|
多克隆位点，限制性内切酶
|
|
载体大小:
|
7697 bp
|
|
5' 测序引物:
|
pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
|
|
3' 测序引物:
|
pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
|
|
载体抗性:
|
氨苄
|
|
筛选标记:
|
Neomycin / G418
|
|
备注:
|
Homo sapiens signal transducer and activator of
transcription 3 (STAT3), transcript variant 1, mRNA. NCBI Reference Sequence:
NM_139276.2
|
|
稳定性:
|
瞬表达
|
|
组成型:
|
组成型
|
|
病毒/非病毒:
|
非病毒
|
载体质粒图谱和多克隆位点信息
全序列
1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG
61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG
121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC
181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT
241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA
301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC
361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC
421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT
481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT
541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA
601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG
661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC
721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG
781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA
841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC
901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCAACAGG ATGGCCCAAT GGAATCAGCT
961 ACAGCAGCTT GACACACGGT ACCTGGAGCA GCTCCATCAG CTCTACAGTG ACAGCTTCCC
1021 AATGGAGCTG CGGCAGTTTC TGGCCCCTTG GATTGAGAGT CAAGATTGGG CATATGCGGC
1081 CAGCAAAGAA TCACATGCCA CTTTGGTGTT TCATAATCTC CTGGGAGAGA TTGACCAGCA
1141 GTATAGCCGC TTCCTGCAAG AGTCGAATGT TCTCTATCAG CACAATCTAC GAAGAATCAA
1201 GCAGTTTCTT CAGAGCAGGT ATCTTGAGAA GCCAATGGAG ATTGCCCGGA TTGTGGCCCG
1261 GTGCCTGTGG GAAGAATCAC GCCTTCTACA GACTGCAGCC ACTGCGGCCC AGCAAGGGGG
1321 CCAGGCCAAC CACCCCACAG CAGCCGTGGT GACGGAGAAG CAGCAGATGC TGGAGCAGCA
1381 CCTTCAGGAT GTCCGGAAGA GAGTGCAGGA TCTAGAACAG AAAATGAAAG TGGTAGAGAA
1441 TCTCCAGGAT GACTTTGATT TCAACTATAA AACCCTCAAG AGTCAAGGAG ACATGCAAGA
1501 TCTGAATGGA AACAACCAGT CAGTGACCAG GCAGAAGATG CAGCAGCTGG AACAGATGCT
1561 CACTGCGCTG GACCAGATGC GGAGAAGCAT CGTGAGTGAG CTGGCGGGGC TTTTGTCAGC
1621 GATGGAGTAC GTGCAGAAAA CTCTCACGGA CGAGGAGCTG GCTGACTGGA AGAGGCGGCA
1681 ACAGATTGCC TGCATTGGAG GCCCGCCCAA CATCTGCCTA GATCGGCTAG AAAACTGGAT
1741 AACGTCATTA GCAGAATCTC AACTTCAGAC CCGTCAACAA ATTAAGAAAC TGGAGGAGTT
1801 GCAGCAAAAA GTTTCCTACA AAGGGGACCC CATTGTACAG CACCGGCCGA TGCTGGAGGA
1861 GAGAATCGTG GAGCTGTTTA GAAACTTAAT GAAAAGTGCC TTTGTGGTGG AGCGGCAGCC
1921 CTGCATGCCC ATGCATCCTG ACCGGCCCCT CGTCATCAAG ACCGGCGTCC AGTTCACTAC
1981 TAAAGTCAGG TTGCTGGTCA AATTCCCTGA GTTGAATTAT CAGCTTAAAA TTAAAGTGTG
2041 CATTGACAAA GACTCTGGGG ACGTTGCAGC TCTCAGAGGA TCCCGGAAAT TTAACATTCT
2101 GGGCACAAAC ACAAAAGTGA TGAACATGGA AGAATCCAAC AACGGCAGCC TCTCTGCAGA
2161 ATTCAAACAC TTGACCCTGA GGGAGCAGAG ATGTGGGAAT GGGGGCCGAG CCAATTGTGA
2221 TGCTTCCCTG ATTGTGACTG AGGAGCTGCA CCTGATCACC TTTGAGACCG AGGTGTATCA
2281 CCAAGGCCTC AAGATTGACC TAGAGACCCA CTCCTTGCCA GTTGTGGTGA TCTCCAACAT
2341 CTGTCAGATG CCAAATGCCT GGGCGTCCAT CCTGTGGTAC AACATGCTGA CCAACAATCC
2401 CAAGAATGTA AACTTTTTTA CCAAGCCCCC AATTGGAACC TGGGATCAAG TGGCCGAGGT
2461 CCTGAGCTGG CAGTTCTCCT CCACCACCAA GCGAGGACTG AGCATCGAGC AGCTGACTAC
2521 ACTGGCAGAG AAACTCTTGG GACCTGGTGT GAATTATTCA GGGTGTCAGA TCACATGGGC
2581 TAAATTTTGC AAAGAAAACA TGGCTGGCAA GGGCTTCTCC TTCTGGGTCT GGCTGGACAA
2641 TATCATTGAC CTTGTGAAAA AGTACATCCT GGCCCTTTGG AACGAAGGGT ACATCATGGG
2701 CTTTATCAGT AAGGAGCGGG AGCGGGCCAT CTTGAGCACT AAGCCTCCAG GCACCTTCCT
2761 GCTAAGATTC AGTGAAAGCA GCAAAGAAGG AGGCGTCACT TTCACTTGGG TGGAGAAGGA
2821 CATCAGCGGT AAGACCCAGA TCCAGTCCGT GGAACCATAC ACAAAGCAGC AGCTGAACAA
2881 CATGTCATTT GCTGAAATCA TCATGGGCTA TAAGATCATG GATGCTACCA ATATCCTGGT
2941 GTCTCCACTG GTCTATCTCT ATCCTGACAT TCCCAAGGAG GAGGCATTCG GAAAGTATTG
3001 TCGGCCAGAG AGCCAGGAGC ATCCTGAAGC TGACCCAGGT AGCGCTGCCC CATACCTGAA
3061 GACCAAGTTT ATCTGTGTGA CACCAACGAC CTGCAGCAAT ACCATTGACC TGCCGATGTC
3121 CCCCCGCACT TTAGATTCAT TGATGCAGTT TGGAAATAAT GGTGAAGGTG CTGAACCCTC
3181 AGCAGGAGGG CAGTTTGAGT CCCTCACCTT TGACATGGAG TTGACCTCGG AGTGCGCTAC
3241 CTCCCCCATG TGACTCGAGT CTAGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT
3301 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA
3361 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG
3421 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA
3481 AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC
3541 CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG
3601 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT
3661 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG
3721 GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA
3781 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC
3841 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC
3901 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA
3961 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA
4021 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT
4081 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC
4141 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
4201 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA
4261 GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA
4321 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA
4381 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG
4441 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT
4501 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC
4561 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG
4621 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG
4681 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA
4741 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA
4801 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT
4861 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC
4921 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC
4981 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG
5041 GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT
5101 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG
5161 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA
5221 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT
5281 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG
5341 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT
5401 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA
5461 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA
5521 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA
5581 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG
5641 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT
5701 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
5761 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG
5821 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA
5881 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
5941 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
6001 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
6061 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
6121 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
6181 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
6241 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
6301 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
6361 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
6421 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
6481 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT
6541 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG
6601 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA
6661 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG
6721 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG
6781 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC
6841 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG
6901 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG
6961 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG
7021 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT
7081 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC
7141 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC
7201 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA
7261 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC
7321 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT
7381 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC
7441 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA
7501 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA
7561 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT
7621 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA
7681 AAGTGCCACC TGACGTC
