测序及更多信息： https://pan.baidu.com/s/1VW0oamMj0hG8i2nBMeLj1w 密码：s7zl
采购地址：淘宝店铺地址
pET-4CDS载体基本信息
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出品公司:
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海吉浩格生物 HedgehogBio
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别名:
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pET-4CDS
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质粒类型:
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大肠杆菌蛋白四表达载体，有四个多克隆位点
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表达水平:
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高
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克隆方法:
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多克隆位点，限制性内切酶
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载体大小:
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5912
bp
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MCS1 5' 测序引物及序列:
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pET
Upstream, GATCCCGCGAAATTAATACG
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MCS2 5' 测序引物及序列:
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M13F, GTAAAACGACGGCCAGT
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MCS3 5' 测序引物及序列:
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M13R, CAGGAAACAGCTATGAC
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MCS4 5' 测序引物及序列:
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PCMV-F,
TCTAAAAGCTGCGGAATTGT
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载体标签:
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N-Flag，N-Strep-Tag II,N-S-Tag,C-6xHis
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载体抗性:
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卡那
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表达宿主菌:
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BL21(DE3) 、 Rosetta2(DE3)、C43(DE3)
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备注:
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pET-4CDS是设计用来同时表达四个目的基因。
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稳定性:
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瞬时表达
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组成型:
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组成型
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病毒/非病毒:
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非病毒
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全序列
1 GCCCCAGCAG GCGAAAATCC TGTTTGATGG TGGTTAACGG CGGGATATAA CATGAGCTGT
61 CTTCGGTATC GTCGTATCCC ACTACCGAGA TATCCGCACC AACGCGCAGC CCGGACTCGG
121 TAATGGCGCG CATTGCGCCC AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA
181 CGATGCCCTC ATTCAGCATT TGCATGGTTT GTTGAAAACC GGACATGGCA CTCCAGTCGC
241 CTTCCCGTTC CGCTATCGGC TGAATTTGAT TGCGAGTGAG ATATTTATGC CAGCCAGCCA
301 GACGCAGACG CGCCGAGACA GAACTTAATG GGCCCGCTAA CAGCGCGATT TGCTGGTGAC
361 CCAATGCGAC CAGATGCTCC ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC
421 TGTTGATGGG TGTCTGGTCA GAGACATCAA GAAATAACGC CGGAACATTA GTGCAGGCAG
481 CTTCCACAGC AATGGCATCC TGGTCATCCA GCGGATAGTT AATGATCAGC CCACTGACGC
541 GTTGCGCGAG AAGATTGTGC ACCGCCGCTT TACAGGCTTC GACGCCGCTT CGTTCTACCA
601 TCGACACCAC CACGCTGGCA CCCAGTTGAT CGGCGCGAGA TTTAATCGCC GCGACAATTT
661 GCGACGGCGC GTGCAGGGCC AGACTGGAGG TGGCAACGCC AATCAGCAAC GACTGTTTGC
721 CCGCCAGTTG TTGTGCCACG CGGTTGGGAA TGTAATTCAG CTCCGCCATC GCCGCTTCCA
781 CTTTTTCCCG CGTTTTCGCA GAAACGTGGC TGGCCTGGTT CACCACGCGG GAAACGGTCT
841 GATAAGAGAC ACCGGCATAC TCTGCGACAT CGTATAACGT TACTGGTTTC ACATTCACCA
901 CCCTGAATTG ACTCTCTTCC GGGCGCTATC ATGCCATACC GCGAAAGGTT TTGCGCCATT
961 CGATGGTGTC CGGGATCTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC
1021 AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG
1081 GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC
1141 ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA
1201 GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG
1261 ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG GATAACAATT
1321 CCCCTCTAGC AATAATTTTG TTTAACTTTA AGAAGGAGAC CGCGGATGGG ATCCGAATTC
1381 GTCGACGAGC TCGATTATAA GGATGATGAC GATAAATAAC TAGCATAACC CCTTGGGGCC
1441 TCTAAACGGG TCTTGAGGGG TTTTTTGTGA TGTAAAACGA CGGCCAGTTA ATACGACTCA
1501 CTATAGGGGA ATTGTGAGCG GATAACAATT CCCCTCGAAA TAATTTTGTT TAACTTTAAG
1561 AAGGAGATAT ACTATGGGTA CCAAGCTTAG TACTACCGGT TGGAGCCACC CGCAGTTCGA
1621 AAAGTAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGTGACA
1681 GGAAACAGCT ATGACTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC
1741 CTCGAAATAA TTTTGTTTAA CTTTAAGAAG GAGAGATCAG ATGTCTAGAA CTAGTTACGT
1801 AGCGGCCGCT AAAGAAACCG CTGCTGCGAA ATTTGAACGC CAGCACATGG ACTCGTCTAC
1861 TAGCGCAGCT TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT
1921 GTCTAAAAGC TGCGGAATTG TTAATACGAC TCACTATAGG GGAATTGTGA GCGGATAACA
1981 ATTCCCCTCG AAATAATTTT GTTTAACTTT AAGAAGGAGA TATACCATGG TCCATATGCT
2041 GCAGCTCGAG CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA
2101 AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA
2161 ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATTGG CGAATGGGAC
2221 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT
2281 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG
2341 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT
2401 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA
2461 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
2521 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA
2581 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC
2641 GCGAATTTTA ACAAAATATT AACGTTTACA ATTTCAGGTG GCACTTTTCG GGGAAATGTG
2701 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAAT
2761 TAATTCTTAG AAAAACTCAT CGAGCATCAA ATGAAACTGC AATTTATTCA TATCAGGATT
2821 ATCAATACCA TATTTTTGAA AAAGCCGTTT CTGTAATGAA GGAGAAAACT CACCGAGGCA
2881 GTTCCATAGG ATGGCAAGAT CCTGGTATCG GTCTGCGATT CCGACTCGTC CAACATCAAT
2941 ACAACCTATT AATTTCCCCT CGTCAAAAAT AAGGTTATCA AGTGAGAAAT CACCATGAGT
3001 GACGACTGAA TCCGGTGAGA ATGGCAAAAG TTTATGCATT TCTTTCCAGA CTTGTTCAAC
3061 AGGCCAGCCA TTACGCTCGT CATCAAAATC ACTCGCATCA ACCAAACCGT TATTCATTCG
3121 TGATTGCGCC TGAGCGAGAC GAAATACGCG ATCGCTGTTA AAAGGACAAT TACAAACAGG
3181 AATCGAATGC AACCGGCGCA GGAACACTGC CAGCGCATCA ACAATATTTT CACCTGAATC
3241 AGGATATTCT TCTAATACCT GGAATGCTGT TTTCCCGGGG ATCGCAGTGG TGAGTAACCA
3301 TGCATCATCA GGAGTACGGA TAAAATGCTT GATGGTCGGA AGAGGCATAA ATTCCGTCAG
3361 CCAGTTTAGT CTGACCATCT CATCTGTAAC ATCATTGGCA ACGCTACCTT TGCCATGTTT
3421 CAGAAACAAC TCTGGCGCAT CGGGCTTCCC ATACAATCGA TAGATTGTCG CACCTGATTG
3481 CCCGACATTA TCGCGAGCCC ATTTATACCC ATATAAATCA GCATCCATGT TGGAATTTAA
3541 TCGCGGCCTA GAGCAAGACG TTTCCCGTTG AATATGGCTC ATAACACCCC TTGTATTACT
3601 GTTTATGTAA GCAGACAGTT TTATTGTTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT
3661 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC
3721 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
3781 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
3841 CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
3901 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
3961 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
4021 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
4081 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
4141 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
4201 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
4261 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
4321 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG
4381 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG
4441 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA
4501 CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA
4561 TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG
4621 CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC
4681 GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA
4741 TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA
4801 CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC
4861 TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC
4921 TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG
4981 CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA
5041 CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC
5101 TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC
5161 CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA
5221 CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC
5281 GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC
5341 CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GGGCCGCCAT GCCGGCGATA
5401 ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG
5461 GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG
5521 CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA
5581 AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG
5641 ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGAGATCCCG GTGCCTAATG AGTGAGCTAA
5701 CTTACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG
5761 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCCAGGGT
5821 GGTTTTTCTT TTCACCAGTG AGACGGGCAA CAGCTGATTG CCCTTCACCG CCTGGCCCTG
5881 AGAGAGTTGC AGCAAGCGGT CCACGCTGGT TT
