测序及更多信息：

https://pan.baidu.com/s/1RiMb6b-8ovA9YyE3w9Oz8g 密码：m8ea

采购地址：淘宝店铺地址

载体名称:	pK18mobSacB
质粒类型:
高拷贝/低拷贝:	高拷贝
启动子:	--
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5721kb
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	Kanamycin
筛选标记:	--
备注:	--
稳定性:	--
组成型:	--
病毒/非病毒:	非病毒

Cloning vector allowing mobilization into a wide range of Gramand Gram+ bacteria. After mobilization, the plasmid can be maintained by integration into the host chromosome via homologous recombination. Excision of the intervening plasmid sequence by a double crossover event can be facilitated by selection on medium containing 10% sucrose.The sacB gene has been modified to eliminate the HindIII and EcoRI sites in the coding region. Designation: pK18mobsacB

全序列

1        GCAATTCCGG TTCGCTTGCT GTCCATAAAA CCGCCCAGTC TAGCTATCGC CATGTAAGCC
61       CACTGCAAGC TACCTGCTTT CTCTTTGCGC TTGCGTTTTC CCTTGTCCAG ATAGCCCAGT
121      AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC GTGTTCCGCT
181      TCCTTTAGCA GCCCTTGCGC CCTGAGTGCT TGCGGCAGCG TGAAGCTAGC TTATCGCGCC
241      ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
301      TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
361      TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTTGCATGC CTGCAGGTCG
421      ACTCTAGAGG ATCCCCGGGT ACCGAGCTCG AATTCGTAAT CATGGTCATA GCTGTTTCCT
481      GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT
541      AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC
601      GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
661      AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
721      GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
781      GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
841      CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
901      AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
961      TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
1021     CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT
1081     CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
1141     CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
1201     TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
1261     GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT
1321     ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
1381     AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
1441     AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
1501     GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
1561     CTTTTGGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCC
1621     TGATAGAAAC AGAAGCCACT GGAGCACCTC AAAAACACCA TCATACACTA AATCAGTAAG
1681     TTGGCAGCAT CACCCGACGC ACTTTGCGCC GAATAAATAC CTGTGACGGA AGATCACTTC
1741     GCAGAATAAA TAAATCCTGG TGTCCCTGTT GATACCGGGA AGCCCTGGGC CAACTTTTGG
1801     CGAAAATGAG ACGTTGATCG GCACGTAAGA GGTTCCAACT TTCACCATAA TGAAATAAGA
1861     TCACTACCGG GCGTATTTTT TGAGTTATCG AGATTTTCAG GAGCTGATAG AAACAGAAGC
1921     CACTGGAGCA CCTCAAAAAC ACCATCATAC ACTAAATCAG TAAGTTGGCA GCATCACCCG
1981     ACGCACTTTG CGCCGAATAA ATACCTGTGA CGGAAGATCA CTTCGCAGAA TAAATAAATC
2041     CTGGTGTCCC TGTTGATACC GGGAAGCCCT GGGCCAACTT TTGGCGAAAA TGAGACGTTG
2101     ATCGGCACGT AAGAGGTTCC AACTTTCACC ATAATGAAAT AAGATCACTA CCGGGCGTAT
2161     TTTTTGAGTT ATCGAGATTT TCAGGAGCTC TTTGGCATCG TCTCTCGCCT GTCCCCTCAG
2221     TTCAGTAATT TCCTGCATTT GCCTGTTTCC AGTCGGTAGA TATTCCACAA AACAGCAGGG
2281     AAGCAGCGCT TTTCCGCTGC ATAACCCTGC TTCGGGGTCA TTATAGCGAT TTTTTCGGTA
2341     TATCCATCCT TTTTCGCACG ATATACAGGA TTTTGCCAAA GGGTTCGTGT AGACTTTCCT
2401     TGGTGTATCC AACGGCGTCA GCGGGGCAGG ATAGGTGAAG TAGGCCCACC CGCGAGCGGG
2461     TGTTCCTTCT TCACTGTCCC TTATTCGCAC CTGGCGGTGC TCAACGGGAA TCCTGCTCTG
2521     CGAGGCTGGC CGGCTACCGC CGGCGTAACA GATGAGGGCA AGCGGATGGC TGATGAAACC
2581     AAGCCAACCA GGAAGGGCAG CCCACCTATC AAGGTGTACT GCCTTCCAGA CGAACGAAGA
2641     GCGATTGAGG AAAAGGCGGC GGCGGCCGGC ATGAGCCTGT CGGCCTACCT GCTGGCCGTC
2701     GGCCAGGGCT ACAAAATCAC GGGCGTCGTG GACTATGAGC ACGTCCGCGA GGGCGTCCCG
2761     GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA
2821     TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGCTCGGT ACCATCGGCA TTTTCTTTTG
2881     CGTTTTTATT TGTTAACTGT TAATTGTCCT TGTTCAAGGA TGCTGTCTTT GACAACAGAT
2941     GTTTTCTTGC CTTTGATGTT CAGCAGGAAG CTCGGCGCAA ACGTTGATTG TTTGTCTGCG
3001     TAGAATCCTC TGTTTGTCAT ATAGCTTGTA ATCACGACAT TGTTTCCTTT CGCTTGAGGT
3061     ACAGCGAAGT GTGAGTAAGT AAAGGTTACA TCGTTAGGAT CAAGATCCAT TTTTAACACA
3121     AGGCCAGTTT TGTTCAGCGG CTTGTATGGG CCAGTTAAAG AATTAGAAAC ATAACCAAGC
3181     ATGTAAATAT CGTTAGACGT AATGCCGTCA ATCGTCATTT TTGATCCGCG GGAGTCAGTG
3241     AACAGGTACC ATTTGCCGTT CATTTTAAAG ACGTTCGCGC GTTCAATTTC ATCTGTTACT
3301     GTGTTAGATG CAATCAGCGG TTTCATCACT TTTTTCAGTG TGTAATCATC GTTTAGCTCA
3361     ATCATACCGA GAGCGCCGTT TGCTAACTCA GCCGTGCGTT TTTTATCGCT TTGCAGAAGT
3421     TTTTGACTTT CTTGACGGAA GAATGATGTG CTTTTGCCAT AGTATGCTTT GTTAAATAAA
3481     GATTCTTCGC CTTGGTAGCC ATCTTCAGTT CCAGTGTTTG CTTCAAATAC TAAGTATTTG
3541     TGGCCTTTAT CTTCTACGTA GTGAGGATCT CTCAGCGTAT GGTTGTCGCC TGAGCTGTAG
3601     TTGCCTTCAT CGATGAACTG CTGTACATTT TGATACGTTT TTCCGTCACC GTCAAAGATT
3661     GATTTATAAT CCTCTACACC GTTGATGTTC AAAGAGCTGT CTGATGCTGA TACGTTAACT
3721     TGTGCAGTTG TCAGTGTTTG TTTGCCGTAA TGTTTACCGG AGAAATCAGT GTAGAATAAA
3781     CGGATTTTTC CGTCAGATGT AAATGTGGCT GAACCTGACC ATTCTTGTGT TTGGTCTTTT
3841     AGGATAGAAT CATTTGCATC GAATTTGTCG CTGTCTTTAA AGACGCGGCC AGCGTTTTTC
3901     CAGCTGTCAA TAGAAGTTTC GCCGACTTTT TGATAGAACA TGTAAATCGA TGTGTCATCC
3961     GCATTTTTAG GATCTCCGGC TAATGCAAAG ACGATGTGGT AGCCGTGATA GTTTGCGACA
4021     GTGCCGTCAG CGTTTTGTAA TGGCCAGCTG TCCCAAACGT CCAGGCCTTT TGCAGAAGAG
4081     ATATTTTTAA TTGTGGACGA ATCAAATTCA GAAACTTGAT ATTTTTCATT TTTTTGCTGT
4141     TCAGGGATTT GCAGCATATC ATGGCGTGTA ATATGGGAAA TGCCGTATGT TTCCTTATAT
4201     GGCTTTTGGT TCGTTTCTTT CGCAAACGCT TGAGTTGCGC CTCCTGCCAG CAGTGCGGTA
4261     GTAAAGGTTA ATACTGTTGC TTGTTTTGCA AACTTTTTGA TGTTCATCGT TCATGTCTCC
4321     TTTTTTATGT ACTGTGTTAG CGGTCTGCTT CTTCCAGCCC TCCTGTTTGA AGATGGCAAG
4381     TTAGTTACGC ACAATAAAAA AAGACCTAAA ATATGTAAGG GGTGACGCCA AAGTATACAC
4441     TTTGCCCTTT ACACATTTTA GGTCTTGCCT GCTTTATCAG TAACAAACCC GCGCGATTTA
4501     CTTTTCGACC TCATTCTATT AGACTCTCGT TTGGATTGCA ACTGGTCTAT TTTCCTCTTT
4561     TGTTTGATAG AAAATCATAA AAGGATTTGC AGACTACGGG CCTAAAGAAC TAAAAAATCT
4621     ATCTGTTTCT TTTCATTCTC TGTATTTTTT ATAGTTTCTG TTGCATGGGC ATAAAGTTGC
4681     CTTTTTAATC ACAATTCAGA AAATATCATA ATATCTCATT TCACTAAATA ATAGTGAACG
4741     GCAGGTATAT GTGATGGGTT AAAAAGGATC GATCCTCTAG CGAACCCCAG AGTCCCGCTC
4801     AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC
4861     CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG
4921     TAGCCAACGC TATGTCCTGA TAGCGGTCCG CCACACCCAG CCGGCCACAG TCGATGAATC
4981     CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA
5041     CGAGATCCTC GCCGTCGGGC ATCCGCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA
5101     GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC
5161     GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG
5221     TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG
5281     ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG
5341     TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG
5401     CTGCCTCGTC TTGGAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG
5461     GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG
5521     CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT
5581     CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCAGATCTT GATCCCCTGC
5641     GCCATCAGAT CCTTGGCGGC AAGAAAGCCA TCCAGTTTAC TTTGCAGGGC TTCCCAACCT
5701     TACCAGAGGG CGCCCCAGCT G