测序及更多信息：腺病毒：https://pan.baidu.com/s/1ZoaupemWEX30JG1r88bj8Q 密码：4t4r
采购地址：淘宝店铺地址
名称：
货号、类型：
规格:：
别名：
启动子：
复制子：
克隆菌株：
培养条件：
质粒宿主：
质粒用途：
插入类型：
物种：
原核抗性：Kan
筛选标记：
荧光标记：
载体基本信息
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载体名称:
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pShuttle-IRES-hrGFP-1
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质粒类型:
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腺病毒表达载体，哺乳动物细胞表达载体
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高拷贝/低拷贝:
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低拷贝
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启动子:
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CMV
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克隆方法:
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多克隆位点，限制性内切酶
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载体大小:
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8.9 kb
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5' 测序引物及序列:
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pShuttle-IRES-hrGFP-1-F:
CTCACGGGGATTTCCAAGTC
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3' 测序引物及序列:
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pShuttle-IRES-hrGFP-1-R:
ATGCAGTCGTCGAGGAATTG
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载体标签:
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--
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载体抗性:
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卡那
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备注:
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载体内包含有IRES序列和hrGFP序列。
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稳定性:
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/
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组成型:
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--
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病毒/非病毒:
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腺病毒
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全序列
1 CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG GGGGTGGAGT
61 TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG TAGTAGTGTG GCGGAAGTGT
121 GATGTTGCAA GTGTGGCGGA ACACATGTAA GCGACGGATG TGGCAAAAGT GACGTTTTTG
181 GTGTGCGCCG GTGTACACAG GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG
241 TAAATTTGGG CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
301 AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA GTAATCAATT
361 ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT
421 GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT
481 CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA
541 ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
601 AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT
661 ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG
721 TACATCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT
781 GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC
841 AACTCCGCCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
901 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG CGGCCGCAGT ACTGCTAGCA
961 CTAGTGATAT CCGATCGGTC GACGCCCGGG CCTCGAGGAC TACAAGGATG ACGATGACAA
1021 GGATTACAAA GACGACGATG ATAAGGACTA TAAGGATGAT GACGACAAAT AATAGCAATT
1081 CCTCGACGAC TGCATAGGGT TACCCCCCTC TCCCTCCCCC CCCCCTAACG TTACTGGCCG
1141 AAGCCGCTTG GAATAAGGCC GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC
1201 GTCTTTTGGC AATGTGAGGG CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG
1261 GGGTCTTTCC CCTCTCGCCA AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT
1321 TCCTCTGGAA GCTTCTTGAA GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA
1381 CCCCCCACCT GGCGACAGGT GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC
1441 AAAGGCGGCA CAACCCCAGT GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG
1501 GCTCTCCTCA AGCGTATTCA ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT
1561 GGGATCTGAT CTGGGGCCTC GGTGCACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA
1621 CGTCTAGGCC CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATGG
1681 CCACAACCAT GGTGAGCAAG CAGATCCTGA AGAACACCGG CCTGCAGGAG ATCATGAGCT
1741 TCAAGGTGAA CCTGGAGGGC GTGGTGAACA ACCACGTGTT CACCATGGAG GGCTGCGGCA
1801 AGGGCAACAT CCTGTTCGGC AACCAGCTGG TGCAGATCCG CGTGACCAAG GGCGCCCCCC
1861 TGCCCTTCGC CTTCGACATC CTGAGCCCCG CCTTCCAGTA CGGCAACCGC ACCTTCACCA
1921 AGTACCCCGA GGACATCAGC GACTTCTTCA TCCAGAGCTT CCCCGCCGGC TTCGTGTACG
1981 AGCGCACCCT GCGCTACGAG GACGGCGGCC TGGTGGAGAT CCGCAGCGAC ATCAACCTGA
2041 TCGAGGAGAT GTTCGTGTAC CGCGTGGAGT ACAAGGGCCG CAACTTCCCC AACGACGGCC
2101 CCGTGATGAA GAAGACCATC ACCGGCCTGC AGCCCAGCTT CGAGGTGGTG TACATGAACG
2161 ACGGCGTGCT GGTGGGCCAG GTGATCCTGG TGTACCGCCT GAACAGCGGC AAGTTCTACA
2221 GCTGCCACAT GCGCACCCTG ATGAAGAGCA AGGGCGTGGT GAAGGACTTC CCCGAGTACC
2281 ACTTCATCCA GCACCGCCTG GAGAAGACCT ACGTGGAGGA CGGCGGCTTC GTGGAGCAGC
2341 ACGAGACCGC CATCGCCCAG CTGACCAGCC TGGGCAAGCC CCTGGGCAGC CTGCACGAGT
2401 GGGTGTAATA GGCGGCCATC CGATCCACCG GATCTAGATA ACTGATCATA ATCAGCCATA
2461 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
2521 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
2581 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
2641 GTGGTTTGTC CAAACTCATC AATGTATCTT AACGCGGATC TGGGCGTGGT TAAGGGTGGG
2701 AAAGAATATA TAAGGTGGGG GTCTTATGTA GTTTTGTATC TGTTTTGCAG CAGCCGCCGC
2761 CGCCATGAGC ACCAACTCGT TTGATGGAAG CATTGTGAGC TCATATTTGA CAACGCGCAT
2821 GCCCCCATGG GCCGGGGTGC GTCAGAATGT GATGGGCTCC AGCATTGATG GTCGCCCCGT
2881 CCTGCCCGCA AACTCTACTA CCTTGACCTA CGAGACCGTG TCTGGAACGC CGTTGGAGAC
2941 TGCAGCCTCC GCCGCCGCTT CAGCCGCTGC AGCCACCGCC CGCGGGATTG TGACTGACTT
3001 TGCTTTCCTG AGCCCGCTTG CAAGCAGTGC AGCTTCCCGT TCATCCGCCC GCGATGACAA
3061 GTTGACGGCT CTTTTGGCAC AATTGGATTC TTTGACCCGG GAACTTAATG TCGTTTCTCA
3121 GCAGCTGTTG GATCTGCGCC AGCAGGTTTC TGCCCTGAAG GCTTCCTCCC CTCCCAATGC
3181 GGTTTAAAAC ATAAATAAAA AACCAGACTC TGTTTGGATT TGGATCAAGC AAGTGTCTTG
3241 CTGTCTTTAT TTAGGGGTTT TGCGCGCGCG GTAGGCCCGG GACCAGCGGT CTCGGTCGTT
3301 GAGGGTCCTG TGTATTTTTT CCAGGACGTG GTAAAGGTGA CTCTGGATGT TCAGATACAT
3361 GGGCATAAGC CCGTCTCTGG GGTGGAGGTA GCACCACTGC AGAGCTTCAT GCTGCGGGGT
3421 GGTGTTGTAG ATGATCCAGT CGTAGCAGGA GCGCTGGGCG TGGTGCCTAA AAATGTCTTT
3481 CAGTAGCAAG CTGATTGCCA GGGGCAGGCC CTTGGTGTAA GTGTTTACAA AGCGGTTAAG
3541 CTGGGATGGG TGCATACGTG GGGATATGAG ATGCATCTTG GACTGTATTT TTAGGTTGGC
3601 TATGTTCCCA GCCATATCCC TCCGGGGATT CATGTTGTGC AGAACCACCA GCACAGTGTA
3661 TCCGGTGCAC TTGGGAAATT TGTCATGTAG CTTAGAAGGA AATGCGTGGA AGAACTTGGA
3721 GACGCCCTTG TGACCTCCAA GATTTTCCAT GCATTCGTCC ATAATGATGG CAATGGGCCC
3781 ACGGGCGGCG GCCTGGGCGA AGATATTTCT GGGATCACTA ACGTCATAGT TGTGTTCCAG
3841 GATGAGATCG TCATAGGCCA TTTTTACAAA GCGCGGGCGG AGGGTGCCAG ACTGCGGTAT
3901 AATGGTTCCA TCCGGCCCAG GGGCGTAGTT ACCCTCACAG ATTTGCATTT CCCACGCTTT
3961 GAGTTCAGAT GGGGGGATCA TGTCTACCTG CGGGGCGATG AAGAAAACGG TTTCCGGGGT
4021 AGGGGAGATC AGCTGGGAAG AAAGCAGGTT CCTGAGCAGC TGCGACTTAC CGCAGCCGGT
4081 GGGCCCGTAA ATCACACCTA TTACCGGCTG CAACTGGTAG TTAAGAGAGC TGCAGCTGCC
4141 GTCATCCCTG AGCAGGGGGG CCACTTCGTT AAGCATGTCC CTGACTCGCA TGTTTTCCCT
4201 GACCAAATCC GCCAGAAGGC GCTCGCCGCC CAGCGATAGC AGTTCTTGCA AGGAAGCAAA
4261 GTTTTTCAAC GGTTTGAGAC CGTCCGCCGT AGGCATGCTT TTGAGCGTTT GACCAAGCAG
4321 TTCCAGGCGG TCCCACAGCT CGGTCACCTG CTCTACGGCA TCTCGATCCA GCATATCTCC
4381 TCGTTTCGCG GGTTGGGGCG GCTTTCGCTG TACGGCAGTA GTCGGTGCTC GTCCAGACGG
4441 GCCAGGGTCA TGTCTTTCCA CGGGCGCAGG GTCCTCGTCA GCGTAGTCTG GGTCACGGTG
4501 AAGGGGTGCG CTCCGGGCTG CGCGCTGGCC AGGGTGCGCT TGAGGCTGGT CCTGCTGGTG
4561 CTGAAGCGCT GCCGGTCTTC GCCCTGCGCG TCGGCCAGGT AGCATTTGAC CATGGTGTCA
4621 TAGTCCAGCC CCTCCGCGGC GTGGCCCTTG GCGCGCAGCT TGCCCTTGGA GGAGGCGCCG
4681 CACGAGGGGC AGTGCAGACT TTTGAGGGCG TAGAGCTTGG GCGCGAGAAA TACCGATTCC
4741 GGGGAGTAGG CATCCGCGCC GCAGGCCCCG CAGACGGTCT CGCATTCCAC GAGCCAGGTG
4801 AGCTCTGGCC GTTCGGGGTC AAAAACCAGG TTTCCCCCAT GCTTTTTGAT GCGTTTCTTA
4861 CCTCTGGTTT CCATGAGCCG GTGTCCACGC TCGGTGACGA AAAGGCTGTC CGTGTCCCCG
4921 TATACAGACT TGAGAGGGAG TTTAAACGAA TTCAATAGCT TGTTGCATGG GCGGCGATAT
4981 AAAATGCAAG GTGCTGCTCA AAAAATCAGG CAAAGCCTCG CGCAAAAAAG AAAGCACATC
5041 GTAGTCATGC TCATGCAGAT AAAGGCAGGT AAGCTCCGGA ACCACCACAG AAAAAGACAC
5101 CATTTTTCTC TCAAACATGT CTGCGGGTTT CTGCATAAAC ACAAAATAAA ATAACAAAAA
5161 AACATTTAAA CATTAGAAGC CTGTCTTACA ACAGGAAAAA CAACCCTTAT AAGCATAAGA
5221 CGGACTACGG CCATGCCGGC GTGACCGTAA AAAAACTGGT CACCGTGATT AAAAAGCACC
5281 ACCGACAGCT CCTCGGTCAT GTCCGGAGTC ATAATGTAAG ACTCGGTAAA CACATCAGGT
5341 TGATTCACAT CGGTCAGTGC TAAAAAGCGA CCGAAATAGC CCGGGGGAAT ACATACCCGC
5401 AGGCGTAGAG ACAACATTAC AGCCCCCATA GGAGGTATAA CAAAATTAAT AGGAGAGAAA
5461 AACACATAAA CACCTGAAAA ACCCTCCTGC CTAGGCAAAA TAGCACCCTC CCGCTCCAGA
5521 ACAACATACA GCGCTTCCAC AGCGGCAGCC ATAACAGTCA GCCTTACCAG TAAAAAAGAA
5581 AACCTATTAA AAAAACACCA CTCGACACGG CACCAGCTCA ATCAGTCACA GTGTAAAAAA
5641 GGGCCAAGTG CAGAGCGAGT ATATATAGGA CTAAAAAATG ACGTAACGGT TAAAGTCCAC
5701 AAAAAACACC CAGAAAACCG CACGCGAACC TACGCCCAGA AACGAAAGCC AAAAAACCCA
5761 CAACTTCCTC AAATCGTCAC TTCCGTTTTC CCACGTTACG TCACTTCCCA TTTTAAGAAA
5821 ACTACAATTC CCAACACATA CAAGTTACTC CGCCCTAAAA CCTACGTCAC CCGCCCCGTT
5881 CCCACGCCCC GCGCCACGTC ACAAACTCCA CCCCCTCATT ATCATATTGG CTTCAATCCA
5941 AAATAAGGTA TATTATTGAT GATGTTAATT AACATGCATG GATCCATATG CGGTGTGAAA
6001 TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA
6061 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
6121 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
6181 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
6241 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
6301 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
6361 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
6421 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
6481 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
6541 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG
6601 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA
6661 GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG
6721 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC
6781 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT
6841 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA
6901 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT
6961 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA
7021 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC
7081 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG
7141 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG
7201 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT
7261 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTGC AGCCATGAGA TTATCAAAAA
7321 GGATCTTCAC CTAGATCCTT TTCACGTAGA AAGCCAGTCC GCAGAAACGG TGCTGACCCC
7381 GGATGAATGT CAGCTACTGG GCTATCTGGA CAAGGGAAAA CGCAAGCGCA AAGAGAAAGC
7441 AGGTAGCTTG CAGTGGGCTT ACATGGCGAT AGCTAGACTG GGCGGTTTTA TGGACAGCAA
7501 GCGAACCGGA ATTGCCAGCT GGGGCGCCCT CTGGTAAGGT TGGGAAGCCC TGCAAAGTAA
7561 ACTGGATGGC TTTCTTGCCG CCAAGGATCT GATGGCGCAG GGGATCAAGC TCTGATCAAG
7621 AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG
7681 CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG
7741 ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC
7801 TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA
7861 CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC
7921 TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG
7981 TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT
8041 TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG
8101 TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA
8161 GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT
8221 TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG
8281 GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG
8341 GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC
8401 GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAAT TTTGTTAAAA TTTTTGTTAA
8461 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCACCA TCCCTTATAA ATCAAAAGAA
8521 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
8581 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
8641 CCATCACCCT AATCAAGTTT TTTGTGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT
8701 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA
8761 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC
8821 GTAACCACCA CACCCGCGCG CTTAATGCGC CGCTACAGGG CGCGTCCATT CGCCATTCAG
8881 GATCGAATTA ATTCTTAATT AA
