测序及更多信息： https://pan.baidu.com/s/10gVnVd-JFoGehSZoa5fATA
提取码：nl97
采购地址：淘宝店铺地址
载体基本信息
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载体名称:
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pSH47
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质粒类型:
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酵母基因敲除载体
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高拷贝/低拷贝:
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高拷贝
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启动子:
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GAL
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克隆方法:
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多克隆位点，限制性内切酶
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载体大小:
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6979
bp
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5' 测序引物及序列:
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M13-F:TGTAAAACGACGGCCAGT
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3' 测序引物及序列:
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M13-R:CAGGAAACAGCTATGAC
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载体筛选标记:
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URA3
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载体抗性:
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氨苄
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Genbank No. :
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AF298782
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备注:
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Cre酶酵母表达质粒
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稳定性:
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稳定
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组成型:
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诱导型
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病毒/非病毒:
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非病毒
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载体质粒图谱和多克隆位点信息
载体简介
pSH47质粒转化到大肠杆菌中时抗性是氨苄。质粒包含有cre重组基因，该基因位于GAL启动子调控下。同时，pSH47载体质粒上面含有ura3酿酒酵母筛选标记。pSH47载体质粒可以用于半乳糖诱导的cre酶表达。
全序列
1 AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG
61 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT
121 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG
181 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT
241 GCACCATACC ACAGCTTTTC AATTCAATTC ATCATTTTTT TTTTATTCTT TTTTTTGATT
301 TCGGTTTCTT TGAAATTTTT TTGATTCGGT AATCTCCGAA CAGAAGGAAG AACGAAGGAA
361 GGAGCACAGA CTTAGATTGG TATATATACG CATATGTAGT GTTGAAGAAA CATGAAATTG
421 CCCAGTATTC TTAACCCAAC TGCACAGAAC AAAAACCTGC AGGAAACGAA GATAAATCAT
481 GTCGAAAGCT ACATATAAGG AACGTGCTGC TACTCATCCT AGTCCTGTTG CTGCCAAGCT
541 ATTTAATATC ATGCACGAAA AGCAAACAAA CTTGTGTGCT TCATTGGATG TTCGTACCAC
601 CAAGGAATTA CTGGAGTTAG TTGAAGCATT AGGTCCCAAA ATTTGTTTAC TAAAAACACA
661 TGTGGATATC TTGACTGATT TTTCCATGGA GGGCACAGTT AAGCCGCTAA AGGCATTATC
721 CGCCAAGTAC AATTTTTTAC TCTTCGAAGA CAGAAAATTT GCTGACATTG GTAATACAGT
781 CAAATTGCAG TACTCTGCGG GTGTATACAG AATAGCAGAA TGGGCAGACA TTACGAATGC
841 ACACGGTGTG GTGGGCCCAG GTATTGTTAG CGGTTTGAAG CAGGCGGCAG AAGAAGTAAC
901 AAAGGAACCT AGAGGCCTTT TGATGTTAGC AGAATTGTCA TGCAAGGGCT CCCTATCTAC
961 TGGAGAATAT ACTAAGGGTA CTGTTGACAT TGCGAAGAGC GACAAAGATT TTGTTATCGG
1021 CTTTATTGCT CAAAGAGACA TGGGTGGAAG AGATGAAGGT TACGATTGGT TGATTATGAC
1081 ACCCGGTGTG GGTTTAGATG ACAAGGGAGA CGCATTGGGT CAACAGTATA GAACCGTGGA
1141 TGATGTGGTC TCTACAGGAT CTGACATTAT TATTGTTGGA AGAGGACTAT TTGCAAAGGG
1201 AAGGGATGCT AAGGTAGAGG GTGAACGTTA CAGAAAAGCA GGCTGGGAAG CATATTTGAG
1261 AAGATGCGGC CAGCAAAACT AAAAAACTGT ATTATAAGTA AATGCATGTA TACTAAACTC
1321 ACAAATTAGA GCTTCAATTT AATTATATCA GTTATTACCC TATGCGGTGT GAAATACCGC
1381 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGAAATTGTA AACGTTAATA TTTTGTTAAA
1441 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA
1501 AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA
1561 CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA
1621 GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG
1681 TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC
1741 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC
1801 AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA
1861 GGGCGCGTCG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG
1921 GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG
1981 GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AGCGCGCGTA
2041 ATACGACTCA CTATAGGGCG AATTGGGTAC CGGCCGCAAA TTAAAGCCTT CGAGCGTCCC
2101 AAAACCTTCT CAAGCAAGGT TTTCAGTATA ATGTTACATG CGTACACGCG TCTGTACAGA
2161 AAAAAAAGAA AAATTTGAAA TATAAATAAC GTTCTTAATA CTAACATAAC TATAAAAAAA
2221 TAAATAGGGA CCTAGACTTC AGGTTGTCTA ACTCCTTCCT TTTCGGTTAG AGCGGATGTG
2281 GGGGGAGGGC GTGAATGTAA GCGTGACATA ACTAATTACA TGACTCGAGG TCGACAATTC
2341 CAACCTTACC CAAGAGTTCG CCAAACTCAG ACATCACTTT AGCAAAACCG CGCCGTGCTT
2401 CTTCCTCGGT GGCATTCATC ACGAAATGTT CAGCACTACG CATACTTTTG ACAGGAAACG
2461 CAACGGATAT TGAGTCAATA TCAGGCATTC TATCGCTCAG CTTTACAGTG ACAATGACGG
2521 CTGGCGACTG AATATTAGTG CTTACAGACA GCACTACATA TTTTCCGTCG ATGTTGAAAT
2581 CCTTTCTCAT ATGTCACCAT AAATATCAAA TAATTATAGC AATCATTTAC GCGTTAATGG
2641 CTAATCGCCA TCTTCCAGCA GGCGCACCAT TGCCCCTGTT TCACTATCCA GGTTACGGAT
2701 ATAGTTCATG ACAATATTTA CATTGGTCCA GCCACCAGCT TGCATGATCT CCGGTATTGA
2761 AACTCCAGCG CGGGCCATAT CTCGCGCGGC TCCGACACGG GCACTGTGTC CAGACCAGGC
2821 CAGGTATCTC TGACCAGAGT CATCCTTAGC GCCGTAAATC AATCGATGAG TTGCTTCAAA
2881 AATCCCTTCC AGGGCGCGAG TTGATAGCTG GCTGGTGGCA GATGGCGCGG CAACACCATT
2941 TTTTCTGACC CGGCAAAACA GGTAGTTATT CGGATCATCA GCTACACCAG AGACGGAAAT
3001 CCATCGCTCG ACCAGTTTAG TTACCCCCAG GCTAAGTGCC TTCTCTACAC CTGCGGTGCT
3061 AACCAGCGTT TTCGTTCTGC CAATATGGAT TAACATTCTC CCACCGTCAG TACGTGAGAT
3121 ATCTTTAACC CTGATCCTGG CAATTTCGGC TATACGTAAC AGGGTGTTAT AAGCAATCCC
3181 CAGAAATGCC AGATTACGTA TATCCTGGCA GCGATCGCTA TTTTCCATGA GTGAACGAAC
3241 CTGGTCGAAA TCAGTGCGTT CGAACGCTAG AGCCTGTTTT GCACGTTCAC CGGCATCAAC
3301 GTTTTCTTTT CGGATCCGCC GCATAACCAG TGAAACAGCA TTGCTGTCAC TTGGTCGTGG
3361 CAGCCCGGAC CGACGATGAA GCATGTTTAG CTGGCCCAAA TGTTGCTGGA TAGTTTTTAC
3421 TGCCAGACCG CGCGCCTGAA GATATAGAAG ATAATCGCGA ACATCTTCAG GTTCTGCGGG
3481 AAACCATTTC CGGTTATTCA ACTTGCACCA TGCCGCCCAC GACCGGCAAA CGGACAGAAG
3541 CATTTTCCAG GTATGCTCAG AAAACGCCTG GCGATCCCTG AACATGTCCA TCAGGTTCTT
3601 GCGAACCTCA TCACTCGTTG CATCGACCGG TAATGCAGGC AAATTTTGGT GTACGGTCAG
3661 TAAATTGGAC ATTTAACACT CAGATAATGG TTTTAAGTAA AGTGTACAGG ATCGGCTCTG
3721 CCCCTCGACG GTATCGATAA GCTTGATATC GAATTCCTGC AGCCCGGGGG ATCCACTAGT
3781 TCTAGAATCC GGGGTTTTTT CTCCTTGACG TTAAAGTATA GAGGTATATT AACAATTTTT
3841 TGTTGATACT TTTATTACAT TTGAATAAGA AGTAATACAA ACCGAAAATG TTGAAAGTAT
3901 TAGTTAAAGT GGTTATGCAG TTTTTGCATT TATATATCTG TTAATAGATC AAAAATCATC
3961 GCTTCGCTGA TTAATTACCC CAGAAATAAG GCTAAAAAAC TAATCGCATT ATCATCCTAT
4021 GGTTGTTAAT TTGATTCGTT CATTTGAAGG TTTGTGGGGC CAGGTTACTG CCAATTTTTC
4081 CTCTTCATAA CCATAAAAGC TAGTATTGTA GAATCTTTAT TGTTCGGAGC AGTGCGGCGC
4141 GAGGCACATC TGCGTTTCAG GAACGCGACC GGTGAAGACG AGGACGCACG GAGGAGAGTC
4201 TTCCTTCGGA GGGCTGTCAC CCGCTCGGCG GCTTCTAATC CGTACTAGAG CTCCAGCTTT
4261 TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
4321 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAG GAGCCGGAAG CATAAAGTGT
4381 AAAGCCTGGG GTGCCTAATG AGTGAGGTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC
4441 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
4501 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
4561 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
4621 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
4681 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
4741 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
4801 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
4861 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
4921 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
4981 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
5041 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
5101 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT
5161 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
5221 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
5281 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
5341 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
5401 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
5461 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
5521 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
5581 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA
5641 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
5701 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
5761 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
5821 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
5881 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
5941 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
6001 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
6061 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
6121 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
6181 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
6241 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
6301 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
6361 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
6421 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGGGT CCTTTTCATC ACGTGCTATA
6481 AAAATAATTA TAATTTAAAT TTTTTAATAT AAATATATAA ATTAAAAATA GAAAGTAAAA
6541 AAAGAAATTA AAGAAAAAAT AGTTTTTGTT TTCCGAAGAT GTAAAAGACT CTAGGGGGAT
6601 CGCCAACAAA TACTACCTTT TATCTTGCTC TTCCTGCTCT CAGGTATTAA TGCCGAATTG
6661 TTTCATCTTG TCTGTGTAGA AGACCACACA CGAAAATCCT GTGATTTTAC ATTTTACTTA
6721 TCGTTAATCG AATGTATATC TATTTAATCT GCTTTTCTTG TCTAATAAAT ATATATGTAA
6781 AGTACGCTTT TTGTTGAAAT TTTTTAAACC TTTGTTTATT TTTTTTTCTT CATTCCGTAA
6841 CTCTTCTACC TTCTTTATTT ACTTTCTAAA ATCCAAATAC AAAACATAAA AATAAATAAA
6901 CACAGAGTAA ATTCCCAAAT TATTCCATCA TTAAAAGATA CGAGGCGCGT GTAAGTTACA
6961 GGCAAGCGAT CCGTCCTA
