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酵母相关 质粒

pSH47 酵母基因敲除载体 Cre酶酵母表达质粒
发布时间:2018-05-28 12:09:23 | 浏览次数:


测序及更多信息: https://pan.baidu.com/s/10gVnVd-JFoGehSZoa5fATA 
提取码:nl97

采购地址:淘宝店铺地址


载体基本信息

载体名称:

pSH47

质粒类型:

酵母基因敲除载体

高拷贝/低拷贝:

高拷贝

启动子:

GAL

克隆方法:

多克隆位点,限制性内切酶

载体大小:

6979 bp

5' 测序引物及序列:

M13-F:TGTAAAACGACGGCCAGT

3' 测序引物及序列:

M13-R:CAGGAAACAGCTATGAC

载体筛选标记:

URA3

载体抗性:

氨苄

Genbank No. :

AF298782

备注:

Cre酶酵母表达质粒

稳定性:

稳定

组成型:

诱导型

病毒/非病毒:

非病毒

载体质粒图谱和多克隆位点信息


载体简介

pSH47质粒转化到大肠杆菌中时抗性是氨苄。质粒包含有cre重组基因,该基因位于GAL启动子调控下。同时,pSH47载体质粒上面含有ura3酿酒酵母筛选标记。pSH47载体质粒可以用于半乳糖诱导的cre酶表达。


全序列

1        AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG
61       TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT
121      CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG
181      TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT
241      GCACCATACC ACAGCTTTTC AATTCAATTC ATCATTTTTT TTTTATTCTT TTTTTTGATT
301      TCGGTTTCTT TGAAATTTTT TTGATTCGGT AATCTCCGAA CAGAAGGAAG AACGAAGGAA
361      GGAGCACAGA CTTAGATTGG TATATATACG CATATGTAGT GTTGAAGAAA CATGAAATTG
421      CCCAGTATTC TTAACCCAAC TGCACAGAAC AAAAACCTGC AGGAAACGAA GATAAATCAT
481      GTCGAAAGCT ACATATAAGG AACGTGCTGC TACTCATCCT AGTCCTGTTG CTGCCAAGCT
541      ATTTAATATC ATGCACGAAA AGCAAACAAA CTTGTGTGCT TCATTGGATG TTCGTACCAC
601      CAAGGAATTA CTGGAGTTAG TTGAAGCATT AGGTCCCAAA ATTTGTTTAC TAAAAACACA
661      TGTGGATATC TTGACTGATT TTTCCATGGA GGGCACAGTT AAGCCGCTAA AGGCATTATC
721      CGCCAAGTAC AATTTTTTAC TCTTCGAAGA CAGAAAATTT GCTGACATTG GTAATACAGT
781      CAAATTGCAG TACTCTGCGG GTGTATACAG AATAGCAGAA TGGGCAGACA TTACGAATGC
841      ACACGGTGTG GTGGGCCCAG GTATTGTTAG CGGTTTGAAG CAGGCGGCAG AAGAAGTAAC
901      AAAGGAACCT AGAGGCCTTT TGATGTTAGC AGAATTGTCA TGCAAGGGCT CCCTATCTAC
961      TGGAGAATAT ACTAAGGGTA CTGTTGACAT TGCGAAGAGC GACAAAGATT TTGTTATCGG
1021     CTTTATTGCT CAAAGAGACA TGGGTGGAAG AGATGAAGGT TACGATTGGT TGATTATGAC
1081     ACCCGGTGTG GGTTTAGATG ACAAGGGAGA CGCATTGGGT CAACAGTATA GAACCGTGGA
1141     TGATGTGGTC TCTACAGGAT CTGACATTAT TATTGTTGGA AGAGGACTAT TTGCAAAGGG
1201     AAGGGATGCT AAGGTAGAGG GTGAACGTTA CAGAAAAGCA GGCTGGGAAG CATATTTGAG
1261     AAGATGCGGC CAGCAAAACT AAAAAACTGT ATTATAAGTA AATGCATGTA TACTAAACTC
1321     ACAAATTAGA GCTTCAATTT AATTATATCA GTTATTACCC TATGCGGTGT GAAATACCGC
1381     ACAGATGCGT AAGGAGAAAA TACCGCATCA GGAAATTGTA AACGTTAATA TTTTGTTAAA
1441     ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA
1501     AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA
1561     CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA
1621     GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG
1681     TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC
1741     GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC
1801     AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA
1861     GGGCGCGTCG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG
1921     GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG
1981     GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AGCGCGCGTA
2041     ATACGACTCA CTATAGGGCG AATTGGGTAC CGGCCGCAAA TTAAAGCCTT CGAGCGTCCC
2101     AAAACCTTCT CAAGCAAGGT TTTCAGTATA ATGTTACATG CGTACACGCG TCTGTACAGA
2161     AAAAAAAGAA AAATTTGAAA TATAAATAAC GTTCTTAATA CTAACATAAC TATAAAAAAA
2221     TAAATAGGGA CCTAGACTTC AGGTTGTCTA ACTCCTTCCT TTTCGGTTAG AGCGGATGTG
2281     GGGGGAGGGC GTGAATGTAA GCGTGACATA ACTAATTACA TGACTCGAGG TCGACAATTC
2341     CAACCTTACC CAAGAGTTCG CCAAACTCAG ACATCACTTT AGCAAAACCG CGCCGTGCTT
2401     CTTCCTCGGT GGCATTCATC ACGAAATGTT CAGCACTACG CATACTTTTG ACAGGAAACG
2461     CAACGGATAT TGAGTCAATA TCAGGCATTC TATCGCTCAG CTTTACAGTG ACAATGACGG
2521     CTGGCGACTG AATATTAGTG CTTACAGACA GCACTACATA TTTTCCGTCG ATGTTGAAAT
2581     CCTTTCTCAT ATGTCACCAT AAATATCAAA TAATTATAGC AATCATTTAC GCGTTAATGG
2641     CTAATCGCCA TCTTCCAGCA GGCGCACCAT TGCCCCTGTT TCACTATCCA GGTTACGGAT
2701     ATAGTTCATG ACAATATTTA CATTGGTCCA GCCACCAGCT TGCATGATCT CCGGTATTGA
2761     AACTCCAGCG CGGGCCATAT CTCGCGCGGC TCCGACACGG GCACTGTGTC CAGACCAGGC
2821     CAGGTATCTC TGACCAGAGT CATCCTTAGC GCCGTAAATC AATCGATGAG TTGCTTCAAA
2881     AATCCCTTCC AGGGCGCGAG TTGATAGCTG GCTGGTGGCA GATGGCGCGG CAACACCATT
2941     TTTTCTGACC CGGCAAAACA GGTAGTTATT CGGATCATCA GCTACACCAG AGACGGAAAT
3001     CCATCGCTCG ACCAGTTTAG TTACCCCCAG GCTAAGTGCC TTCTCTACAC CTGCGGTGCT
3061     AACCAGCGTT TTCGTTCTGC CAATATGGAT TAACATTCTC CCACCGTCAG TACGTGAGAT
3121     ATCTTTAACC CTGATCCTGG CAATTTCGGC TATACGTAAC AGGGTGTTAT AAGCAATCCC
3181     CAGAAATGCC AGATTACGTA TATCCTGGCA GCGATCGCTA TTTTCCATGA GTGAACGAAC
3241     CTGGTCGAAA TCAGTGCGTT CGAACGCTAG AGCCTGTTTT GCACGTTCAC CGGCATCAAC
3301     GTTTTCTTTT CGGATCCGCC GCATAACCAG TGAAACAGCA TTGCTGTCAC TTGGTCGTGG
3361     CAGCCCGGAC CGACGATGAA GCATGTTTAG CTGGCCCAAA TGTTGCTGGA TAGTTTTTAC
3421     TGCCAGACCG CGCGCCTGAA GATATAGAAG ATAATCGCGA ACATCTTCAG GTTCTGCGGG
3481     AAACCATTTC CGGTTATTCA ACTTGCACCA TGCCGCCCAC GACCGGCAAA CGGACAGAAG
3541     CATTTTCCAG GTATGCTCAG AAAACGCCTG GCGATCCCTG AACATGTCCA TCAGGTTCTT
3601     GCGAACCTCA TCACTCGTTG CATCGACCGG TAATGCAGGC AAATTTTGGT GTACGGTCAG
3661     TAAATTGGAC ATTTAACACT CAGATAATGG TTTTAAGTAA AGTGTACAGG ATCGGCTCTG
3721     CCCCTCGACG GTATCGATAA GCTTGATATC GAATTCCTGC AGCCCGGGGG ATCCACTAGT
3781     TCTAGAATCC GGGGTTTTTT CTCCTTGACG TTAAAGTATA GAGGTATATT AACAATTTTT
3841     TGTTGATACT TTTATTACAT TTGAATAAGA AGTAATACAA ACCGAAAATG TTGAAAGTAT
3901     TAGTTAAAGT GGTTATGCAG TTTTTGCATT TATATATCTG TTAATAGATC AAAAATCATC
3961     GCTTCGCTGA TTAATTACCC CAGAAATAAG GCTAAAAAAC TAATCGCATT ATCATCCTAT
4021     GGTTGTTAAT TTGATTCGTT CATTTGAAGG TTTGTGGGGC CAGGTTACTG CCAATTTTTC
4081     CTCTTCATAA CCATAAAAGC TAGTATTGTA GAATCTTTAT TGTTCGGAGC AGTGCGGCGC
4141     GAGGCACATC TGCGTTTCAG GAACGCGACC GGTGAAGACG AGGACGCACG GAGGAGAGTC
4201     TTCCTTCGGA GGGCTGTCAC CCGCTCGGCG GCTTCTAATC CGTACTAGAG CTCCAGCTTT
4261     TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
4321     GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAG GAGCCGGAAG CATAAAGTGT
4381     AAAGCCTGGG GTGCCTAATG AGTGAGGTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC
4441     GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
4501     AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
4561     GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
4621     GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
4681     CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
4741     AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
4801     TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
4861     CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
4921     CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
4981     CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
5041     TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
5101     GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT
5161     ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
5221     AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
5281     AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
5341     GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
5401     CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
5461     GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
5521     TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
5581     GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA
5641     ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
5701     ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
5761     CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
5821     TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
5881     AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
5941     TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
6001     TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
6061     AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
6121     GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
6181     AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
6241     ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
6301     GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
6361     CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
6421     GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGGGT CCTTTTCATC ACGTGCTATA
6481     AAAATAATTA TAATTTAAAT TTTTTAATAT AAATATATAA ATTAAAAATA GAAAGTAAAA
6541     AAAGAAATTA AAGAAAAAAT AGTTTTTGTT TTCCGAAGAT GTAAAAGACT CTAGGGGGAT
6601     CGCCAACAAA TACTACCTTT TATCTTGCTC TTCCTGCTCT CAGGTATTAA TGCCGAATTG
6661     TTTCATCTTG TCTGTGTAGA AGACCACACA CGAAAATCCT GTGATTTTAC ATTTTACTTA
6721     TCGTTAATCG AATGTATATC TATTTAATCT GCTTTTCTTG TCTAATAAAT ATATATGTAA
6781     AGTACGCTTT TTGTTGAAAT TTTTTAAACC TTTGTTTATT TTTTTTTCTT CATTCCGTAA
6841     CTCTTCTACC TTCTTTATTT ACTTTCTAAA ATCCAAATAC AAAACATAAA AATAAATAAA
6901     CACAGAGTAA ATTCCCAAAT TATTCCATCA TTAAAAGATA CGAGGCGCGT GTAAGTTACA
6961     GGCAAGCGAT CCGTCCTA




 
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