昆虫相关质粒

pCoHygro 昆虫表达载体筛选载体

发布时间:2018-05-28 12:04:50 | 浏览次数：

测序及更多信息： https://pan.baidu.com/s/187kLrJNFpnGhzO3bWGZlqg

提取码：hedg

采购地址：淘宝店铺地址

我们的官网 http://hedgehogbio.comhttp://hedgehogbio.com/

载体名称:	pCoHygro
质粒类型:	昆虫表达载体；筛选载体
克隆方法:	--
启动子:	Drosophila copia promoter
载体大小:	4526 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	无
载体抗性:	氨苄青霉素
真核筛选标记:	hygromycin
宿主细胞（系）:	果蝇细胞系S2
备注:	pCoHygro载体昆虫载体共转染，建立稳表达细胞系。
瞬表达/稳表达:	--
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

全序列

1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTTGCATGC CTGCAGGTCG
421 ACTCTAGAGG ATCCGGTGCG TGGTGGTTTC ATGCTTCTGG GAACGGCAAA TGGGTTTAGG
481 ATTGGGAACC CCTCATCATC TGTTGGAATA TACTATTCAA CCTACAAAAA TAACGTTAAA
541 CAACACTACT TTATATTTGA TATGAATGGC CACACCTTTT ATGCCATAAA ACATATTGTA
601 AGAGAATACC ACTCTTTTTA TTCCTTCTTT CCTTCTTGTA CGTTTTTTGC TGTGAGTAGG
661 TCGTGGTGCT GGTGTTGCAG TTGAAATAAC TTAAAATATA AATCATAAAA CTCAAACATA
721 AACTTGACTA TTTATTTATT TATTAAGAAA GGAAATATAA ATTATAAATT ACAACAGGTT
781 ATGGGGATCC CCAGCTTGGC CACCATGGGA AAGCCTGAGC GTACCGCGAC GTCTGTCGAG
841 AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA TGCAGCTCTC GGAGGGCGAA
901 GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT ATGTCCTGCG GGTAAATAGC
961 TGCGGCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC ACTTTGCATC GGCCGCGCTC
1021 CCGATTCCGG AAGTGCTTGA CATTGGGGAA TTCAGCGAGA GCCTGACCTA TTGCATCTCC
1081 CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA CCGAACTGCC CGCTGTTCTG
1141 CAGCCGGTCG CGGAGGCCAT GGATGCGATC GCTGCGGCCG ATCTTAGCCA GACGAGCGGG
1201 TTCGGCCCAT TCGGACCGCA AGGAATCGGT CAATACACTA CATGGCGTGA TTTCATATGC
1261 GCGATTGCTG ATCCCCATGT GTATCACTGG CAAACTGTGA TGGACGACAC CGTCAGTGCG
1321 TCCGTCGCGC AGGCTCTCGA TGAGCTGATG CTTTGGGCCG AGGACTGCCC CGAAGTCCGG
1381 CACCTCGTGC ACGCGGATTT CGGCTCCAAC AATGTCCTGA CGGACAATGG CCGCATAACA
1441 GCGGTCATTG ACTGGAGCGA GGCGATGTTC GGGGATTCCC AATACGAGGT CGCCAACATC
1501 TTCTTCTGGA GGCCGTGGTT GGCTTGTATG GAGCAGCAGA CGCGCTACTT CGAGCGGAGG
1561 CATCCGGAGC TTGCAGGATC GCCGCGGCTC CGGGCGTATA TGCTCCGCAT TGGTCTTGAC
1621 CAACTCTATC AGAGCTTGGT TGACGGCAAT TTCGATGATG CAGCTTGGGC GCAGGGTCGA
1681 TGCGACGCAA TCGTCCGATC CGGAGCCGGG ACTGTCGGGC GTACACAAAT CGCCCGCAGA
1741 AGCGCGGCCG TCTGGACCGA TGGCTGTGTA GAAGTACTCG CCGATAGTGG AAACCGACGC
1801 CCCAGCACTC GTCCGAGGGC AAAGGAATAG AGTAGATGCC GACCGAACAA GAGCTGATTT
1861 CGAGAACGCC TCAGCCAGCA ACTCGCGCGA GCCTAGCAAG GCAAATGCGA GAGAACGGCC
1921 TTACGCTTGG TGGCACAGTT CTCGTCCACA GTTCGCTAAG CTCGCTCGGC TGGGTCGCGG
1981 GAGGGCCGGT CGCAGTGATT CAGGCCCTTC TGGATTGTGT TGGTCCCCAG GGCACGATTG
2041 TCATGCCCAC GCACTCGGGT GATCTGACTG ATCCCGCAGA TTGGAGATCG CCGCCCGTGC
2101 CTGCCGATTG GGTGCAGATC AGCCTCGAGG CCAGCTAGCT TGAACTTGTT TATTGCAGCT
2161 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
2221 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGATCGGG
2281 TACCGAGCTC GAATTCGTAA TCATGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT
2341 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG
2401 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT
2461 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG
2521 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC
2581 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
2641 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT
2701 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
2761 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
2821 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
2881 GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
2941 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
3001 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
3061 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
3121 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
3181 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
3241 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
3301 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
3361 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
3421 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
3481 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
3541 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
3601 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
3661 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
3721 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
3781 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
3841 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
3901 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
3961 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
4021 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
4081 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
4141 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
4201 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
4261 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
4321 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
4381 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC
4441 CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA
4501 TAGGCGTATC ACGAGGCCCT TTCGTC