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其他质粒

pEX18Tc pEX-18Tc 自杀质粒
发布时间:2018-05-28 12:01:53 | 浏览次数:



测序及更多信息:https://pan.baidu.com/s/1ROa5FzATjaPBVgK77oQn5Q 密码:2j72

采购地址:淘宝店铺地址

载体基本信息

出品公司:

载体名称:

pEX18Tc, pEX-18Tc

质粒类型:

自杀质粒

高拷贝/低拷贝:

克隆方法:

限制性内切酶,多克隆位点

启动子:

Lac

载体大小:

6349 bp

5' 测序引物及序列:

M13F(-47) CGCCAGGGTTTTCCCAGTCACGAC

3' 测序引物及序列:

M13R(-48) AGCGGATAACAATTTCACACAGGA

载体抗性:

四环素

病毒/非病毒:

非病毒

 



1        TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
61       CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
121      TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
181      ACCATAATCG GCATTTTCTT TTGCGTTTTT ATTTGTTAAC TGTTAATTGT CCTTGTTCAA
241      GGATGCTGTC TTTGACAACA GATGTTTTCT TGCCTTTGAT GTTCAGCAGG AAGCTAGGCG
301      CAAACGTTGA TTGTTTGTCT GCGTAGAATC CTCTGTTTGT CATATAGCTT GTAATCACGA
361      CATTGTTTCC TTTCGCTTGA GGTACAGCGA AGTGTGAGTA AGTAAAGGTT ACATCGTTAG
421      GATCAAGATC CATTTTTAAC ACAAGGCCAG TTTTGTTCAG CGGCTTGTAT GGGCCAGTTA
481      AAGAATTAGA AACATAACCA AGCATGTAAA TATCGTTAGA CGTAATGCCG TCAATCGTCA
541      TTTTTGATCC GCGGGAGTCA GTGAACAGAT ACCATTTGCC GTTCATTTTA AAGACGTTCG
601      CGCGTTCAAT TTCATCTGTT ACTGTGTTAG ATGCAATCAG CGGTTTCATC ACTTTTTTCA
661      GTGTGTAATC ATCGTTTAGC TCAATCATAC CGAGAGCGCC GTTTGCTAAC TCAGCCGTGC
721      GTTTTTTATC GCTTTGCAGA AGTTTTTGAC TTTCTTGACG GAAGAATGAT GTGCTTTTGC
781      CATAGTATGC TTTGTTAAAT AAAGATTCTT CGCCTTGGTA GCCATCTTCA GTTCCAGTGT
841      TTGCTTCAAA TACTAAGTAT TTGTGGCCTT TATCTTCTAC GTAGTGAGGA TCTCTCAGCG
901      TATGGTTGTC GCCTGAGCTG TAGTTGCCTT CATCGATGAA CTGCTGTACA TTTTGATACG
961      TTTTTCCGTC ACCGTCAAAG ATTGATTTAT AATCCTCTAC ACCGTTGATG TTCAAAGAGC
1021     TGTCTGATGC TGATACGTTA ACTTGTGCAG TTGTCAGTGT TTGTTTGCCG TAATGTTTAC
1081     CGGAGAAATC AGTGTAGAAT AAACGGATTT TTCCGTCAGA TGTAAATGTG GCTGAACCTG
1141     ACCATTCTTG TGTTTGGTCT TTTAGGATAG AATCATTTGC ATCGAATTTG TCGCTGTCTT
1201     TAAAGACGCG GCCAGCGTTT TTCCAGCTGT CAATAGAAGT TTCGCCGACT TTTTGATAGA
1261     ACATGTAAAT CGATGTGTCA TCCGCATTTT TAGGATCTCC GGCTAATGCA AAGACGATGT
1321     GGTAGCCGTG ATAGTTTGCG ACAGTGCCGT CAGCGTTTTG TAATGGCCAG CTGTCCCAAA
1381     CGTCCAGGCC TTTTGCAGAA GAGATATTTT TAATTGTGGA CGAATCGAAC TCAGGAACTT
1441     GATATTTTTC ATTTTTTTGC TGTTCAGGGA TTTGCAGCAT ATCATGGCGT GTAATATGGG
1501     AAATGCCGTA TGTTTCCTTA TATGGCTTTT GGTTCGTTTC TTTCGCAAAC GCTTGAGTTG
1561     CGCCTCCTGC CAGCAGTGCG GTAGTAAAGG TTAATACTGT TGCTTGTTTT GCAAACTTTT
1621     TGATGTTCAT CGTTCATGTC TCCTTTTTTA TGTACTGTGT TAGCGGTCTG CTTCTTCCAG
1681     CCCTCCTGTT TGAAGATGGC AAGTTAGTTA CGCACAATAA AAAAAGACCT AAAATATGTA
1741     AGGGGTGACG CCAAAGTATA CACTTTGCCC TTTACACATT TTAGGTCTTG CCTGCTTTAT
1801     CAGTAACAAA CCCGCGCGAT TTACTTTTCG ACCTCATTCT ATTAGACTCT CGTTTGGATT
1861     GCAACTGGTC TATTTTCCTC TTTTGTTTGA TAGAAAATCA TAAAAGGATT TGCAGACTAC
1921     GGGCCTAAAG AACTAAAAAA TCTATCTGTT TCTTTTCATT CTCTGTATTT TTTATAGTTT
1981     CTGTTGCATG GGCATAAAGT TGCCTTTTTA ATCACAATTC AGAAAATATC ATAATATCTC
2041     ATTTCACTAA ATAATAGTGA ACGGCAGGTA TATGTGATGG GTTAAAAAGG ATCGATCCTC
2101     TAGCTAGAGT CGATCTTCGC CAGCAGGGCG AGGATCGTGG CATCACCGAA CCGCGCCGTG
2161     CGCGGGTCGT CGGTGAGCCA GAGTTTCAGC AGGCCGCCCA GGCGGCCCAG GTCGCCATTG
2221     ATGCGGGCCA GCTCGCGGAC GTGCTCATAG TCCACGACGC CCGTGATTTT GTAGCCCTGG
2281     CCGACGGCCA GCAGGTAGGC CGACAGGCTC ATGCCGGCCG CCGCCGCCTT TTCCTCAATC
2341     GCTCTTCGTT CGTCTGGAAG GCAGTACACC TTGATAGGTG GGCTGCCCTT CCTGGTTGGC
2401     TTGGTTTCAT CAGCCATCCG CTTGCCCTCA TCTGTTACGC CGGCGGTAGC CGGCCAGCCT
2461     CGCAGAGCAG GATTCCCGTT GAGCACCGCC AGGTGCGAAT AAGGGACAGT GAAGAAGGAA
2521     CACCCGCTCG CGGGTGGGCC TACTTCACCT ATCCTGCCCG GCTGACGCCG TTGGATACAC
2581     CAAGGAAAGT CTACACGAAC CCTTTGGCAA AATCCTGTAT ATCGTGCGAA AAAGGATGGA
2641     TATACCGAAA AAATCGCTAT AATGACCCCG AAGCAGGGTT ATGCAGCGGA AAAGCGCTGC
2701     TTCCCTGCTG TTTTGTGGAA TATCTACCGA CTGGAAACAG GCAAATGCAG GAAATTACTG
2761     AACTGAGGGG ACAGGCGAGA GACGATGCCA AAGAGCTACA CCGACGAGCT GGCCGAGTGG
2821     GTTGAATCCC GCGCGGCCAA GAAGCGCCGG CGTGATGAGG CTGCGGTTGC GTTCCTGGCG
2881     GTGAGGGCGG ATGTCGATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC
2941     CGCATCAGGC GCATATTTGA ATGTATTTAG AAAATAAACA AAAGAGTTTG TAGAAACGCA
3001     AAAAGGCCAT CCGTCAGGAT GGCCTTCTGC TTAATTTGAT GCCTGGCAGT TTATGGCGGG
3061     CGTCCTGCCC GCCACCCTCC GGGCCGTTGC TTCGCAACGT TCAAATCCGC TCCCGGCGGA
3121     TTTGTCCTAC TCAGGAGAGC GTTCACCGAC AAACAACAGA TAAAACGAAA GGCCCAGTCT
3181     TTCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC CCTACTCTCG CATGGGGAGA
3241     CCCCACACTA CCATCGGCGC TACGGCGTTT CACTTCTGAG TTCGGCATGG GGTCAGGTGG
3301     GACCACCGCG CTACTGCCGC CAGGCAAATT CTGTTTTATC AGACCGCTTC TGCGTTCTGA
3361     TTTAATCTGT ATCAGGCTGA AAATCTTCTC TCATCCGCCA AAACAGCCAA GCTCGCCATT
3421     CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC
3481     GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT
3541     CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT TGCATGCCTG CAGGTCGACT
3601     CTAGAGGATC CCCGGGTACC GAGCTCGAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG
3661     TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA
3721     GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT
3781     TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA
3841     GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
3901     GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
3961     TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
4021     AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
4081     AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
4141     CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
4201     TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
4261     AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
4321     GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
4381     TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
4441     ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC
4501     TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
4561     CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
4621     AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
4681     AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
4741     TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
4801     AATCGATGCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA
4861     TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC
4921     CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC
4981     AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG
5041     CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG
5101     ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC
5161     GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA
5221     GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG
5281     TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC
5341     AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA
5401     GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC
5461     GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG
5521     CATCGGTCGG CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT
5581     GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGT AAGGAGATGG CGCCCAACAG
5641     TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA
5701     GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC
5761     TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGAATCCACA GGACGGGTGT
5821     GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG
5881     GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC
5941     ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG
6001     CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT
6061     GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA
6121     ATTTAACTGT GATAAACTAC CGCATTAAAG CTAATCGGTT GAATACTCAT ACTCTTCCTT
6181     TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
6241     TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT
6301     GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG
6361     CCCTTTCGTC




 
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