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PB-rtTA-BsmbI (Plasmid #126028)
发布时间:2022-07-18 18:12:22 | 浏览次数:




  • PURPOSE
    (Empty Backbone) PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning
  • Backbone size (bp)8660
  • Modifications to backbone
    rtTA3-IRES-Neo cassette cloned from pSLIK-Neo (Addgene#25735). U6 expression cassette from pX330 (Addgene#42230) with dual BsmBI sites was cloned into the PacI site upstream of the rtTA3-IRES-Neo cassette.
  • Vector type
    Mammalian Expression, CRISPR ; piggyBac
  • PromoterU6
  • Selectable markers
    Neomycin (select with G418)

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown



A piggyBac-based toolkit for inducible genome editing in mammalian cells. Schertzer MD, Thulson E, Braceros KCA, Lee DM, Hinkle ER, Murphy RM, Kim SO, Vitucci EM, Calabrese JM. RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118. 10.1261/rna.068932.118 PubMed 31101683


 
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