• PURPOSE

  (Empty Backbone) PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning
• Backbone size (bp)8660
• Modifications to backbone
  rtTA3-IRES-Neo cassette cloned from pSLIK-Neo (Addgene#25735). U6 expression cassette from pX330 (Addgene#42230) with dual BsmBI sites was cloned into the PacI site upstream of the rtTA3-IRES-Neo cassette.
• Vector type
  Mammalian Expression, CRISPR ; piggyBac
• PromoterU6
• Selectable markers
  Neomycin (select with G418)

• Bacterial Resistance(s)
  Ampicillin, 100 μg/mL
• Growth Temperature
  30°C
• Growth Strain(s)
  DH5alpha
• Copy number
  Unknown

A piggyBac-based toolkit for inducible genome editing in mammalian cells. Schertzer MD, Thulson E, Braceros KCA, Lee DM, Hinkle ER, Murphy RM, Kim SO, Vitucci EM, Calabrese JM. RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118. 10.1261/rna.068932.118 PubMed 31101683