pET-28b-Cas9-His 
(Plasmid #47327)

载体作用

For in vitro expression and purification of Cas9 protein

载体骨架

pET-28b

构建方法

· Cloning methodRestriction Enzyme

· 5′ cloning siteNcoI (not destroyed)

· 3′ cloning siteNotI (not destroyed)

载体类型

细菌表达， CRISPR

载体抗性

Kanamycin

筛选标记

-

插入基因信息

Cas9

参考文献

Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, Montague TG, Zimmerman S, Richter C, Schier AF. PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014. PONE-D-14-14210 [pii] PubMed 24873830