GENE/INSERT

• Gene/Insert name
  Hepatitis B virus protein X
• Alt name
  HBx
• Species
  Hepatitis B virus
• Insert Size (bp)
  492
• PromoterCMV
• Tag / Fusion Protein
  • Flag (N terminal on insert)

CLONING INFORMATION

• Cloning methodRestriction Enzyme
• 5′ cloning siteNheI (not destroyed)
• 3′ cloning siteEcoRV (not destroyed)
• 5′ sequencing primerCMV-F
• 3′ sequencing primerBGH-rev