测序及更多信息： https://pan.baidu.com/s/1azl_djtu24oGI8PhbDIDXA?pwd=hedg

采购地址：淘宝店铺地址

• PURPOSE

  Expresses nCas9(D10A)-PmCDA1 in yeast cells

• Vector backbone
  pRS315
• Backbone size w/o insert (bp)6000
• Total vector size (bp)12005
• Modifications to backbone
  EcoRI site is mutated in Leu2
• Vector type
  Yeast Expression
• Selectable markers
  LEU2

• Gene/Insert name
  SpCas9
• Species
  Streptococcus pyogenes
• Mutation
  D10A for nickase Cas9
• PromoterpGal1
• Tag / Fusion Protein
  • PmCDA1 (C terminal on insert)

参考文献

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A. Science. 2016 Aug 4. pii: aaf8729. 10.1126/science.aaf8729 PubMed 27492474