真核编辑质粒

pX333 CRISPR双靶点基因编辑质粒 CAS9 双sgRNA

发布时间:2020-10-30 16:36:16 | 浏览次数：

测序及更多信息： https://pan.baidu.com/s/1azl_djtu24oGI8PhbDIDXA?pwd=hedg

采购地址：淘宝店铺地址

PURPOSE

(Empty Backbone) All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

参考文献

In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura A. Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22. 10.1038/nature13902 PubMed 25337876