• PURPOSE

  Inducible TtAgo expression

• 

• • Vector backbone
    pCDF-1b
     (Search Vector Database)
  • Backbone manufacturer
    EMD Millipore
  • Backbone size w/o insert (bp)3621
  • Vector type
    Bacterial Expression

  GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

  
  

GENE/INSERT

• Gene/Insert name
  TtAgo
• Alt name
  TTHB068
• Species
  Thermus thermophilus
• Insert Size (bp)
  2223
• GenBank ID
  AP008227
• PromoterT7
• Tag / Fusion Protein
  • Strep-II tag (N terminal on insert)

CLONING INFORMATION

• Cloning methodRestriction Enzyme
• 5′ cloning siteNcoI (destroyed during cloning)
• 3′ cloning siteAvrII (not destroyed)
• 5′ sequencing primerT7
• 3′ sequencing primerT7-Term

(Common Sequencing Primers)



DNA-guided DNA interference by a prokaryotic Argonaute. Swarts DC, Jore MM, Westra ER, Zhu Y, Janssen JH, Snijders AP, Wang Y, Patel DJ, Berenguer J, Brouns SJ, van der Oost J. Nature. 2014 Mar 13;507(7491):258-61. doi: 10.1038/nature12971. Epub 2014 Feb 16. 10.1038/nature12971 PubMed 24531762