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pWUR702(Plasmid #53079) TtAgo诱导表达载体HH-Y-043
发布时间:2020-08-18 13:03:18 | 浏览次数:


  • PURPOSE

    Inducible TtAgo expression

    • Vector backbone
      pCDF-1b
    • Backbone manufacturer
      EMD Millipore
    • Backbone size w/o insert (bp)3621
    • Vector type
      Bacterial Expression

    GROWTH IN BACTERIA

    • Bacterial Resistance(s)
      Spectinomycin
    • Growth Temperature
      37°C
    • Growth Strain(s)
      DH5alpha
    • Copy number
      High Copy

GENE/INSERT

  • Gene/Insert name
    TtAgo
  • Alt name
    TTHB068
  • Species
    Thermus thermophilus
  • Insert Size (bp)
    2223
  • GenBank ID
    AP008227
  • PromoterT7
  • Tag / Fusion Protein
    • Strep-II tag (N terminal on insert)

CLONING INFORMATION

  • Cloning methodRestriction Enzyme
  • 5′ cloning siteNcoI (destroyed during cloning)
  • 3′ cloning siteAvrII (not destroyed)
  • 5′ sequencing primerT7
  • 3′ sequencing primerT7-Term

    (Common Sequencing Primers)


    DNA-guided DNA interference by a prokaryotic Argonaute. Swarts DC, Jore MM, Westra ER, Zhu Y, Janssen JH, Snijders AP, Wang Y, Patel DJ, Berenguer J, Brouns SJ, van der Oost J. Nature. 2014 Mar 13;507(7491):258-61. doi: 10.1038/nature12971. Epub 2014 Feb 16. 10.1038/nature12971 PubMed 24531762








 
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