| pEM-Cas9HF1-recA56（Plasmid #89962） |
| 发布时间:2020-05-16 13:36:02 | 浏览次数： |
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测序及更多信息：https://pan.baidu.com/s/1Uh2zgjYZKZGrumoYMivOLA?pwd=hedg 采购地址：淘宝店铺地址
PURPOSE
Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.
Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity. Moreb EA, Hoover B, Yaseen A, Valyasevi N, Roecker Z, Menacho-Melgar R, Lynch MD. ACS Synth Biol. 2017 Oct 2. doi: 10.1021/acssynbio.7b00174. 10.1021/acssynbio.7b00174 PubMed 28915012
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