测序及更多信息：https://pan.baidu.com/s/1Uh2zgjYZKZGrumoYMivOLA?pwd=hedg

采购地址：淘宝店铺地址

• Vector backbone
  pX330
  
  
• Backbone manufacturer
  Zhang lab (Addgene plasmid # 42230)
• Modifications to backbone
  The T2A-mCherry fragment was cloned into FseI & EcoRI sites of pX330 (Addgene plasmid # 42230). The mCherry template was derived from MSCV-IRES-mCherry, a kind gift of Frank Rosenbauer and Martin Janz (Charite, Berlin)
• Vector type
  Mammalian Expression, CRISPR

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. 10.1038/nbt.3198 PubMed 25803306

pU6-(BbsI)_CBh-Cas9-T2A-mCherry(Plasmid #64324)