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pYES2-NTA 酿酒酵母蛋白表达载体 N端Tag
发布时间:2019-08-01 21:30:25 | 浏览次数:



测序及更多信息:https://pan.baidu.com/s/1VbhalcAMelfnxWQOTnbeoA 
提取码:v9aq 

采购地址:淘宝店铺地址

我们的官网  http://hedgehogbio.comhttp://hedgehogbio.com/


载体基本信息

载体名称:

pYES2-NTA, pYES2/NT A

质粒类型:

酿酒酵母蛋白表达载体

表达水平:

高拷贝

诱导方法:

半乳糖

启动子:

GAL1

克隆方法:

多克隆位点,限制性内切酶

载体大小:

6038 bp

5' 测序引物及序列:

T7: TAATACGACTCACTATAGGG

3' 测序引物及序列:

CYC1 Terminator: GTGACATAACTAATTACATGATG

载体标签:

N-His, N-Xpress, C-His, C-V5

载体抗性:

氨苄

筛选标记:

URA3

备注:

利用半乳糖诱导蛋白在酿酒酵母中表达。

稳定性:

稳定 Stable

组成型/诱导型:

诱导型

病毒/非病毒:

非病毒



全序列


1        ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
61       CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
121      ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
181      CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
241      TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
301      TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
361      GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
421      CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
481      GACTCACTAT AGGGAATATT AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG
541      GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA
601      AGGTACCTAG GATCCAGTGT GGTGGAATTC TGCAGATATC CAGCACAGTG GCGGCCGCTC
661      GAGTCTAGAG GGCCCTTCGA AGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT
721      ACGCGTACCG GTCATCATCA CCATCACCAT TGAGTTTAAA CCCGCTGATC CTAGAGGGCC
781      GCATCATGTA ATTAGTTATG TCACGCTTAC ATTCACGCCC TCCCCCCACA TCCGCTCTAA
841      CCGAAAAGGA AGGAGTTAGA CAACCTGAAG TCTAGGTCCC TATTTATTTT TTTATAGTTA
901      TGTTAGTATT AAGAACGTTA TTTATATTTC AAATTTTTCT TTTTTTTCTG TACAGACGCG
961      TGTACGCATG TAACATTATA CTGAAAACCT TGCTTGAGAA GGTTTTGGGA CGCTCGAAGG
1021     CTTTAATTTG CAAGCTGCGG CCCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG
1081     TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG
1141     GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG
1201     GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA
1261     GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
1321     ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
1381     TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
1441     CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
1501     GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
1561     CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
1621     ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
1681     GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC
1741     TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
1801     CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
1861     ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
1921     ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
1981     TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
2041     CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
2101     TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG CGCTTACCAT CTGGCCCCAG
2161     TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
2221     GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
2281     TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
2341     TGTTGGCATT GCTACAGGCA TCGTGGTGTC ACTCTCGTCG TTTGGTATGG CTTCATTCAG
2401     CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
2461     TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
2521     GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
2581     GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
2641     TTGCCCGGCG TCAATACGGG ATAATAGTGT ATCACATAGC AGAACTTTAA AAGTGCTCAT
2701     CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
2761     TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
2821     TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
2881     GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATGGGTA ATAACTGATA TAATTAAATT
2941     GAAGCTCTAA TTTGTGAGTT TAGTATACAT GCATTTACTT ATAATACAGT TTTTTAGTTT
3001     TGCTGGCCGC ATCTTCTCAA ATATGCTTCC CAGCCTGCTT TTCTGTAACG TTCACCCTCT
3061     ACCTTAGCAT CCCTTCCCTT TGCAAATAGT CCTCTTCCAA CAATAATAAT GTCAGATCCT
3121     GTAGAGACCA CATCATCCAC GGTTCTATAC TGTTGACCCA ATGCGTCTCC CTTGTCATCT
3181     AAACCCACAC CGGGTGTCAT AATCAACCAA TCGTAACCTT CATCTCTTCC ACCCATGTCT
3241     CTTTGAGCAA TAAAGCCGAT AACAAAATCT TTGTCGCTCT TCGCAATGTC AACAGTACCC
3301     TTAGTATATT CTCCAGTAGA TAGGGAGCCC TTGCATGACA ATTCTGCTAA CATCAAAAGG
3361     CCTCTAGGTT CCTTTGTTAC TTCTTCTGCC GCCTGCTTCA AACCGCTAAC AATACCTGGG
3421     CCCACCACAC CGTGTGCATT CGTAATGTCT GCCCATTCTG CTATTCTGTA TACACCCGCA
3481     GAGTACTGCA ATTTGACTGT ATTACCAATG TCAGCAAATT TTCTGTCTTC GAAGAGTAAA
3541     AAATTGTACT TGGCGGATAA TGCCTTTAGC GGCTTAACTG TGCCCTCCAT GGAAAAATCA
3601     GTCAAGATAT CCACATGTGT TTTTAGTAAA CAAATTTTGG GACCTAATGC TTCAACTAAC
3661     TCCAGTAATT CCTTGGTGGT ACGAACATCC AATGAAGCAC ACAAGTTTGT TTGCTTTTCG
3721     TGCATGATAT TAAATAGCTT GGCAGCAACA GGACTAGGAT GAGTAGCAGC ACGTTCCTTA
3781     TATGTAGCTT TCGACATGAT TTATCTTCGT TTCCTGCAGG TTTTTGTTCT GTGCAGTTGG
3841     GTTAAGAATA CTGGGCAATT TCATGTTTCT TCAACACTAC ATATGCGTAT ATATACCAAT
3901     CTAAGTCTGT GCTCCTTCCT TCGTTCTTCC TTCTGTTCGG AGATTACCGA ATCAAAAAAA
3961     TTTCAAAGAA ACCGAAATCA AAAAAAAGAA TAAAAAAAAA ATGATGAATT GAATTGAAAA
4021     GCTAGCTTAT CGATGATAAG CTGTCAAAGA TGAGAATTAA TTCCACGGAC TATAGACTAT
4081     ACTAGATACT CCGTCTACTG TACGATACAC TTCCGCTCAG GTCCTTGTCC TTTAACGAGG
4141     CCTTACCACT CTTTTGTTAC TCTATTGATC CAGCTCAGCA AAGGCAGTGT GATCTAAGAT
4201     TCTATCTTCG CGATGTAGTA AAACTAGCTA GACCGAGAAA GAGACTAGAA ATGCAAAAGG
4261     CACTTCTACA ATGGCTGCCA TCATTATTAT CCGATGTGAC GCTGCAGCTT CTCAATGATA
4321     TTCGAATACG CTTTGAGGAG ATACAGCCTA ATATCCGACA AACTGTTTTA CAGATTTACG
4381     ATCGTACTTG TTACCCATCA TTGAATTTTG AACATCCGAA CCTGGGAGTT TTCCCTGAAA
4441     CAGATAGTAT ATTTGAACCT GTATAATAAT ATATAGTCTA GCGCTTTACG GAAGACAATG
4501     TATGTATTTC GGTTCCTGGA GAAACTATTG CATCTATTGC ATAGGTAATC TTGCACGTCG
4561     CATCCCCGGT TCATTTTCTG CGTTTCCATC TTGCACTTCA ATAGCATATC TTTGTTAACG
4621     AAGCATCTGT GCTTCATTTT GTAGAACAAA AATGCAACGC GAGAGCGCTA ATTTTTCAAA
4681     CAAAGAATCT GAGCTGCATT TTTACAGAAC AGAAATGCAA CGCGAAAGCG CTATTTTACC
4741     AACGAAGAAT CTGTGCTTCA TTTTTGTAAA ACAAAAATGC AACGCGACGA GAGCGCTAAT
4801     TTTTCAAACA AAGAATCTGA GCTGCATTTT TACAGAACAG AAATGCAACG CGAGAGCGCT
4861     ATTTTACCAA CAAAGAATCT ATACTTCTTT TTTGTTCTAC AAAAATGCAT CCCGAGAGCG
4921     CTATTTTTCT AACAAAGCAT CTTAGATTAC TTTTTTTCTC CTTTGTGCGC TCTATAATGC
4981     AGTCTCTTGA TAACTTTTTG CACTGTAGGT CCGTTAAGGT TAGAAGAAGG CTACTTTGGT
5041     GTCTATTTTC TCTTCCATAA AAAAAGCCTG ACTCCACTTC CCGCGTTTAC TGATTACTAG
5101     CGAAGCTGCG GGTGCATTTT TTCAAGATAA AGGCATCCCC GATTATATTC TATACCGATG
5161     TGGATTGCGC ATACTTTGTG AACAGAAAGT GATAGCGTTG ATGATTCTTC ATTGGTCAGA
5221     AAATTATGAA CGGTTTCTTC TATTTTGTCT CTATATACTA CGTATAGGAA ATGTTTACAT
5281     TTTCGTATTG TTTTCGATTC ACTCTATGAA TAGTTCTTAC TACAATTTTT TTGTCTAAAG
5341     AGTAATACTA GAGATAAACA TAAAAAATGT AGAGGTCGAG TTTAGATGCA AGTTCAAGGA
5401     GCGAAAGGTG GATGGGTAGG TTATATAGGG ATATAGCACA GAGATATATA GCAAAGAGAT
5461     ACTTTTGAGC AATGTTTGTG GAAGCGGTAT TCGCAATGGG AAGCTCCACC CCGGTTGATA
5521     ATCAGAAAAG CCCCAAAAAC AGGAAGATTG TATAAGCAAA TATTTAAATT GTAAACGTTA
5581     ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACGAATAGC
5641     CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG
5701     TTCCAGTTTC CAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA
5761     AAAGGGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG
5821     GGTCGAGGTG CCGTAAAGCA GTAAATCGGA AGGGTAAACG GATGCCCCCA TTTAGAGCTT
5881     GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGGG
5941     CTAGGGCGGT GGGAAGTGTA GGGGTCACGC TGGGCGTAAC CACCACACCC GCCGCGCTTA
6001     ATGGGGCGCT ACAGGGCGCG TGGGGATGAT CCACTAGT




 
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