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PURPOSE
Mammalian expression of ultrasensitive protein calcium sensor
参考文献
Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, Baohan A, Schreiter ER, Kerr RA, Orger MB, Jayaraman V, Looger LL, Svoboda K, Kim DS. Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354. 10.1038/nature12354 PubMed 23868258
全序列
1 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA
61 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT
121 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT
181 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT
241 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC
301 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT
361 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
421 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA
481 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA
541 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT
601 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC
661 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT
721 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA
781 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG
841 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA
901 TTAACGCTTA CAATTTACGC CTTAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA
961 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC
1021 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
1081 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
1141 GATTATGATC TAGAGTCGCG GCCGCTCACT TCGCTGTCAT CATTTGTACA AACTCTTCGT
1201 AGTTTACCTG ACCATCCCCA TCGATGTCTG CTTCCCTGAT CATTTCATCA ACCTCTTCAT
1261 CTGTTAACTT CTCTCCAAGG TTTGTCATCA CGTGGCGAAG CTCTGCTGCA CTGATGTAGC
1321 CATTGCCATC CTTATCAAAC ACACCGAACG CTTCTCTAAT TTCTTCTTCC GTGTCCCTGT
1381 ATTTCATTTT TCTTGCCATC ATTGTCAGGA ACTCAGGGAA GTCGATTGTG CCGTCACCGT
1441 CGGCATCTAC TTCATTGATC ATGTCCTGCA GCTCTGCTTC TGTGGGGTTC TGCCCCAGAG
1501 ACCGCATCAC CGTCCCCAGC TCCTTGGTTG TTATTGTCCC ATCCCCGTCC TTGTCAAATA
1561 GGGAGAATTC CTCTTTAAAT TCTGCGATCT GCTCTTCAGT CAGTTGGTCC GGCAGGTTGT
1621 ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA
1681 TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT
1741 CGTCCTTGAA GAAGATGGTG CGCTCCTGGA TGTAGCCTTC GGGCATGGCG GACTTGAAGA
1801 AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGGTCAGGG
1861 TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATG AACTTCAGGG
1921 TCAGCTTGCC GTAGGTGGCA TCGCCCTCAC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT
1981 TTACGTCGCC GTCCAGCTCG ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT
2041 TGCTCACCAT GCTCCCTCCG GTACCGCCCT TGTACAGCTC GTCCATGCCG AGAGTGATCC
2101 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTCG
2161 AAAGTTTGGA CTGCACGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA
2221 TGGGGGTGTT CTGCTGGTAG TGGTAGGCGA GCTGCACGCC GCCGTCCTCG ATGTTGTGGC
2281 GGATCTTGAA GTTCGCCTTG ATGCCGTTCT TCTGCTTGTC GGCCTTGATA TAGACGTTCT
2341 CGAGTGAGCT CAGCCGACCT ATAGCTCTGA CTGCGTGACC TGTCTTATTC CACTTACGAC
2401 GTGATGAGTC GACCATGGTG GCGAGATCCT TATCGTCATC GTCGTACAGA TCCCGACCCA
2461 TTTGCTGTCC ACCAGTCATG CTAGCCATAC CATGATGATG ATGATGATGA GAACCCATGG
2521 TGGCGAGATC TGAGTCCGGT AGCGCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT
2581 TATATAGACC TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT
2641 ACGACATTTT GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT
2701 GGGGTGGAGA CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG
2761 CCAAAACCGC ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCAAGTAGG
2821 AAAGTCCCAT AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC
2881 GTCAATAGGG GGCGTACTTG GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA
2941 CCGTAAATAC TCCACCCATT GACGTCAATG GAAAGTCCCT ATTGGCGTTA CTATGGGAAC
3001 ATACGTCATT ATTGACGTCA ATGGGCGGGG GTCGTTGGGC GGTCAGCCAG GCGGGCCATT
3061 TACCGTAAGT TATGTAACGC GGAACTCCAT ATATGGGCTA TGAACTAATG ACCCCGTAAT
3121 TGATTACTAT TAATAACTAA TGCATGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA
3181 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG
3241 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT
3301 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA
3361 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
3421 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT
3481 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
3541 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
3601 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
3661 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC
3721 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG
3781 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC
3841 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT
3901 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA
3961 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG
4021 CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG
4081 AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT
4141 GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT
4201 TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT
4261 CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA
4321 TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG
4381 TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA
4441 GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG
4501 AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC
4561 AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGGTCC GCCACACCCA GCCGGCCACA
4621 GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC
4681 ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC
4741 GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC
4801 CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC
4861 CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG
4921 AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT
4981 TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA
5041 CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC
5101 AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT
5161 TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC
5221 GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT
5281 TGCAAAAGCC TAGGCCTCCA AAAAAGCC