测序及更多信息:https://pan.baidu.com/s/1_8Ughx08VsESQNYk7YgX-w 提取码:l1ey
采购地址:淘宝店铺地址
我们的官网 http://hedgehogbio.comhttp://hedgehogbio.com/
pGL3-Rluc
出品公司:
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海吉浩格生物 HedgehogBio
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载体名称:
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pGL3-Rluc
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质粒类型:
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哺乳细胞表达,信号通路报告载体 萤火虫荧光luc+和海肾荧光hRluc
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高拷贝/低拷贝:
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高
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启动子:
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无启动子
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克隆方法:
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多克隆位点,限制性内切酶
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载体大小:
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6162bp
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5' 测序引物及序列:
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RVP3:CTAGCAAAATAGGCTGTCCC
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3' 测序引物及序列:
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--
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载体标签:
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--
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载体抗性:
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氨苄青霉素(Ampicillin)
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筛选标记:
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--
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备注:
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pGL3-Rluc载体含萤火虫荧光素酶报告基因luc+和海参荧光素酶报告基因hRluc,无启动子,用于研究目的启动子的功能,不需要同时转染对照载体pRL-TK。
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稳定性:
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--
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组成型:
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组成型
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病毒/非病毒:
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非病毒
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全序列
1 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
61 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT
121 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
181 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
241 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
301 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
361 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
421 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
481 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
541 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT
601 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
661 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
721 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
781 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
841 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
901 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
961 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
1021 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
1081 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
1141 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
1201 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
1261 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
1321 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
1381 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG
1441 CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC
1501 CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC
1561 CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT
1621 CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC
1681 GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC
1741 TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT
1801 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA
1861 AATATTAACG TTTACAATTT CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG
1921 ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCCCAAGCTA CCATGATAAG TAAGTAATAT
1981 TAAGGTACGG GAGGTACTTG GAGCGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG
2041 TGTGTTGGTT TTTTGTGTGA ATCGATAGTA CTAACATACG CTCTCCATCA AAACAAAACG
2101 AAACAAAACA AACTAGCAAA ATAGGCTGTC CCCAGTGCAA GTGCAGGTGC CAGAACATTT
2161 CTCTATCGAT AGGTACCGAG CTCTTACGCG TGCTAGCCCG GGCTCGAGAT CTGCGATCTA
2221 AGTAAGCTTG GCATTCCGGT ACTGTTGGTA AAGCCACCAT GGAAGACGCC AAAAACATAA
2281 AGAAAGGCCC GGCGCCATTC TATCCGCTGG AAGATGGAAC CGCTGGAGAG CAACTGCATA
2341 AGGCTATGAA GAGATACGCC CTGGTTCCTG GAACAATTGC TTTTACAGAT GCACATATCG
2401 AGGTGGACAT CACTTACGCT GAGTACTTCG AAATGTCCGT TCGGTTGGCA GAAGCTATGA
2461 AACGATATGG GCTGAATACA AATCACAGAA TCGTCGTATG CAGTGAAAAC TCTCTTCAAT
2521 TCTTTATGCC GGTGTTGGGC GCGTTATTTA TCGGAGTTGC AGTTGCGCCC GCGAACGACA
2581 TTTATAATGA ACGTGAATTG CTCAACAGTA TGGGCATTTC GCAGCCTACC GTGGTGTTCG
2641 TTTCCAAAAA GGGGTTGCAA AAAATTTTGA ACGTGCAAAA AAAGCTCCCA ATCATCCAAA
2701 AAATTATTAT CATGGATTCT AAAACGGATT ACCAGGGATT TCAGTCGATG TACACGTTCG
2761 TCACATCTCA TCTACCTCCC GGTTTTAATG AATACGATTT TGTGCCAGAG TCCTTCGATA
2821 GGGACAAGAC AATTGCACTG ATCATGAACT CCTCTGGATC TACTGGTCTG CCTAAAGGTG
2881 TCGCTCTGCC TCATAGAACT GCCTGCGTGA GATTCTCGCA TGCCAGAGAT CCTATTTTTG
2941 GCAATCAAAT CATTCCGGAT ACTGCGATTT TAAGTGTTGT TCCATTCCAT CACGGTTTTG
3001 GAATGTTTAC TACACTCGGA TATTTGATAT GTGGATTTCG AGTCGTCTTA ATGTATAGAT
3061 TTGAAGAAGA GCTGTTTCTG AGGAGCCTTC AGGATTACAA GATTCAAAGT GCGCTGCTGG
3121 TGCCAACCCT ATTCTCCTTC TTCGCCAAAA GCACTCTGAT TGACAAATAC GATTTATCTA
3181 ATTTACACGA AATTGCTTCT GGTGGCGCTC CCCTCTCTAA GGAAGTCGGG GAAGCGGTTG
3241 CCAAGAGGTT CCATCTGCCA GGTATCAGGC AAGGATATGG GCTCACTGAG ACTACATCAG
3301 CTATTCTGAT TACACCCGAG GGGGATGATA AACCGGGCGC GGTCGGTAAA GTTGTTCCAT
3361 TTTTTGAAGC GAAGGTTGTG GATCTGGATA CCGGGAAAAC GCTGGGCGTT AATCAAAGAG
3421 GCGAACTGTG TGTGAGAGGT CCTATGATTA TGTCCGGTTA TGTAAACAAT CCGGAAGCGA
3481 CCAACGCCTT GATTGACAAG GATGGATGGC TACATTCTGG AGACATAGCT TACTGGGACG
3541 AAGACGAACA CTTCTTCATC GTTGACCGCC TGAAGTCTCT GATTAAGTAC AAAGGCTATC
3601 AGGTGGCTCC CGCTGAATTG GAATCCATCT TGCTCCAACA CCCCAACATC TTCGACGCAG
3661 GTGTCGCAGG TCTTCCCGAC GATGACGCCG GTGAACTTCC CGCCGCCGTT GTTGTTTTGG
3721 AGCACGGAAA GACGATGACG GAAAAAGAGA TCGTGGATTA CGTCGCCAGT CAAGTAACAA
3781 CCGCGAAAAA GTTGCGCGGA GGAGTTGTGT TTGTGGACGA AGTACCGAAA GGTCTTACCG
3841 GAAAACTCGA CGCAAGAAAA ATCAGAGAGA TCCTCATAAA GGCCAAGAAG GGCGGAAAGA
3901 TCGCCGTGTA ATTCTAGAGT CGGGGCGGCC GGCCGCTTCG AGCAGACATG ATAAGATACA
3961 TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA
4021 TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA
4081 ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA
4141 AGTAAAACCT CTACAAATGT GGTAAAATCG ATAAGGATCC TCACTGCTCG TTCTTCAGCA
4201 CGCGCTCCAC GAAGCTCTTG ATGTACTTAC CCATTTCATC TGGAGCGTCC TCCTGGCTGA
4261 AGTGGAGGCC CTTCACCTTC ACGAACTCGG TGTTAGGGAA CTTCTTAGCT CCCTCGACAA
4321 TAGCGTTGGA AAAGAACCCA GGGTCGGACT CGATGAACAT CTTAGGCAGA TCGTCGCTGG
4381 CCCGAAGGTA GGCGTTGTAG TTGCGGACAA TCTGGACGAC GTCGGGCTTG CCTCCCTTAA
4441 CGAGAGGGAT CTCGCGAGGC CAGGAGAGGG TAGGCCGTCT AACCTCGCCC TTCTCCTTGA
4501 ATGGCTCCAG GTAGGCAGCG AACTCCTCAG GCTCCAGTTT CCGCATGATC TTGCTTGGGA
4561 GCATGGTCTC GACGAAGAAG TTATTCTCAA GCACCATTTT CTCGCCCTCT TCGCTCTTGA
4621 TCAGGGCGAT ATCCTCCTCG ATGTCAGGCC ACTCGTCCCA GGACTCGATC ACGTCCACGA
4681 CACTCTCAGC ATGGACGATG GCCTTGATCT TGTCTTGGTG CTCGTAGGAG TAGTGAAAGG
4741 CCAGACAAGC CCCCCAGTCG TGGCCCACAA AGATGATTTT CTTTGGAAGG TTCAGCAGCT
4801 CGAACCAAGC GGTGAGGTAC TTGTAGTGAT CCAGGAGGCG ATATGAGCCA TTCCCGCTCT
4861 TGCCGGACTT ACCCATTCCG ATCAGATCAG GGATGATGCA TCTAGCCACG GGCTCGATGT
4921 GAGGCACGAC GTGCCTCCAC AGGTAGCTGG AGGCAGCGTT ACCATGCAGA AAAATCACGG
4981 CGTTCTCGGC GTGCTTCTCG GAATCATAGT AGTTGATGAA GGAGTCCAGC ACGTTCATTT
5041 GCTTGCAGCG AGCCCACCAC TGAGGCCCAG TGATCATGCG TTTGCGTTGC TCGGGGTCGT
5101 ACACCTTGGA AGCCATGGTG GCTCCAGCCT TTGGTTCGAG ACTGTTGTGT CAGAAGAATC
5161 AAGCTTTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC
5221 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG
5281 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT
5341 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA
5401 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG
5461 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC
5521 TCAGGTCGAC CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG
5581 GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG
5641 GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
5701 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
5761 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
5821 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
5881 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
5941 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
6001 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT
6061 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
6121 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AG