测序和更多信息: https://pan.baidu.com/s/1FsvFhY2rY8C9dqKpsHzo_g 密码: u54s
采购地址：淘宝店铺地址
pCBC-MT1T2
(Plasmid
#50593)
载体作用
CRISPR/Cas based plant genome
editing and gene regulation; used as template for making expression cassette
with multiple gRNA target sites
载体骨架
pGWC
构建方法
载体类型
Plant Expression, CRISPR ; PCR
template
载体抗性
Chloramphenicol
筛选标记
插入基因信息
T1, gRNA
scaffold, OsU3t plus, TaU3p, T2
参考文献
A CRISPR/Cas9
toolkit for multiplex genome editing in plants. Xing HL, Dong L,
Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ. BMC Plant Biol. 2014 Nov
29;14(1):327. 10.1186/s12870-014-0327-y PubMed 25432517
全序列
1 GGTTTTCATG GCTTGTTATG ACTGTTTTTT TGGGGTACAG TCTATGCCTC GGGCATCCAA
61 GCAGCAAGCG CGTTACGCCG TGGGTCGATG TTTGATGTTA TGGAGCAGCA ACGATGTTAC
121 GCAGCAGGGC AGTCGCCCTA AAACAAAGTT AAACATCATG GAGAAAAAAA TCACTGGATA
181 TACCACCGTT GATATATCCC AATGGCATCG TAAAGAACAT TTTGAGGCAT TTCAGTCAGT
241 TGCTCAATGT ACCTATAACC AGACCGTTCA GCTGGATATT ACGGCCTTTT TAAAGACCGT
301 AAAGAAAAGT AAGCACAAGT TTTATCCGGC CTTTATTCAC ATTCTTGCCC GCCTGATGAA
361 TGCTCATCCG GAGTTCCGTA TGGCAATGAA AGACGGTGAG CTGGTGATAT GGGATAGTGT
421 TCACCCTTGT TACACCGTTT TCCATGAGCA AACTGAAACG TTTTCATCGC TCTGGAGTGA
481 ATACCACGAC GATTTCCGGC AGTTTCTACA CATATATTCG CAAGATGTGG CGTGTTACGG
541 TGAAAACCTG GCCTATTTCC CTAAAGGGTT TATTGAGAAT ATGTTTTTCG TCTCAGCCAA
601 TCCCTGGGTG AGTTTCACCA GTTTTGATTT AAACGTGGCC AATATGGACA ACTTCTTCGC
661 CCCCGTTTTC ACAATGGGCA AATATTATAC GCAAGGCGAC AAGGTGCTGA TGCCGCTGGC
721 GATTCAGGTT CATCATGCCG TTTGTGATGG CTTCCATGTC GGCAGAATGC TTAATGAATT
781 ACAACAGTAC TGCGATGAGT GGCAGGGCGG GGCGTAATTT TTTTAAGGCA GTTATTGGTG
841 CGATCCTCTA GCTAGAAATT CGTTCAAGCC GACGCCGCTT CGCGGCGCGG CTTAACTCAA
901 GCGTTAGATG CACTAAGCAC ATAATTGCTC ACAGCCAAAC TATCAGGTCA AGTCCCGCGG
961 AGTTGTTCGG TAAATTGTCA CAACGCCGCC CGCCCCGTTC CACTGAGCGT CAGACCCGGT
1021 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
1081 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
1141 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA
1201 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
1261 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
1321 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
1381 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA
1441 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
1501 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
1561 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
1621 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
1681 TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCTAG
1741 GATGGATCTC GGGCACGTCT AACTACTAAG CGAGAGTAGG GAACTGCCAG GCATCAAATA
1801 AAACGAAAGG CTCAGTCGGA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC
1861 GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGTGAA GCAACGGCCC
1921 GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAACTAA GCAGAAGGCC
1981 ATCCTGACGG ATGGCCTTTT TGCGTTTCTA CAAACTCTTC CTGTTAGTTA GTTACTTAAG
2041 TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA
2101 CTAAACCGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC
2161 TATTTAGGTG ACACTATAGA ATACTCAAGC TATGCAAGGC CCTTAAGGCC AAGGGGCTTC
2221 GTGCCAACCA ACGAGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT
2281 CAACTTGAAA AAGTGGCACC GAGTCGGTGC TTTTTTTTTT CGTTTTGCAT TGAGTTTTCT
2341 CCGTCGCATG TTTGCAGTTT TATTTTCCGT TTTGCATTGA AATTTCTCCG TCTCATGTTT
2401 GCAGCGTGTT CAAAAAGTAC GCAGCTGTAT TTCACTTATT TACGGCGCCA CATTTTCATG
2461 CCGTTTGTGC CAACTATCCC GAGCTAGTGA ATACAGCTTG GCTTCACACA ACACTGGTGA
2521 CCCGCTGACC TGCTCGTACC TCGTACCGTC GTACGGCACA GCATTTGGAA TTAAAGGGTG
2581 TGATCGATAC TGCTTGCTGC TCATGAATCC AAACCACACG GAGTTCAAAT TCCCACAGAT
2641 TAAGGCTCGT CCGTCGCACA AGGTAATGTG TGAATATTAT ATCTGTCGTG CAAAATTGCC
2701 TGGCCTGCAC AATTGCTGTT ATAGTTGGCG GCAGGGAGAG TTTTAACATT GACTAGCGTG
2761 CTGATAATTT GTGAGAAATA ATAATTGACA AGTAGATACT GACATTTGAG AAGAGCTTCT
2821 GAACTGTTAT TAGTAACAAA AATGGAAAGC TGATGCACGG AAAAAGGAAA GAAAAAGCCA
2881 TACTTTTTTT TAGGTAGGAA AAGAAAAAGC CATACGAGAC TGATGTCTCT CAGATGGGCC
2941 GGGATCTGTC TATCTAGCAG GCAGCAGCCC ACCAACCTCA CGGGCCAGCA ATTACGAGTC
3001 CTTCTAAAAG CTCCCGCCGA GGGGCGCTGG CGCTGCTGTG CAGCAGCACG TCTAACATTA
3061 GTCCCACCTC GCCAGTTTAC AGGGAGCAGA ACCAGCTTAT AAGCGGAGGC GCGGCACCAA
3121 GAAGCGTGAC CAGGAGGAGC ATGCCCCTTT GGCCTATTTG GCCTGGCCCA ATTCGCCCTA
3181 TAGTGAGTCG TATTACAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC
3241 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG
3301 CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTATACG TACGGCAGTT
3361 TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG TTTGTGGATG TACAGAGTGA
3421 TATTATTGAC ACGCCGGGGC GACGGATGGT GATCCCCCTG GCCAGTGCAC GTCTGCTGTC
3481 AGATAAAGTC TCCCGTGAAC TTTACCCGGT GGTGCATATC GGGGATGAAA GCTGGCGCAT
3541 GATGACCACC GATATGGCAA GTGTGCCAGT ATCCGTTATC GGGGAAGAAG TGGCTGATCT
3601 CAGCCACCGC GAAAATGACA TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT
3661 GTCAGGCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTCACGT AGAAAGCCAG
3721 TCCGCAGAAA CGGACCATGA GTGGACATAA GCCTCGTTCG GTTCGTAAGC TGTAATGCAA
3781 GTAGCGTATG CGCTCACGCA ACTGGTCCAG AACCTTGACC GAACGCAGCG GTGGTAACGG
3841 CGCAGTGGC
