测序和更多信息: https://pan.baidu.com/s/1FsvFhY2rY8C9dqKpsHzo_g 密码: u54s 

采购地址：淘宝店铺地址

pCBC-MT1T2 
(Plasmid #50593)

载体作用

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

载体骨架

pGWC

构建方法

载体类型

Plant Expression, CRISPR ; PCR template

载体抗性

Chloramphenicol

筛选标记

插入基因信息

T1, gRNA scaffold, OsU3t plus, TaU3p, T2

参考文献

A CRISPR/Cas9 toolkit for multiplex genome editing in plants. Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ. BMC Plant Biol. 2014 Nov 29;14(1):327. 10.1186/s12870-014-0327-y PubMed 25432517

全序列

1        GGTTTTCATG GCTTGTTATG ACTGTTTTTT TGGGGTACAG TCTATGCCTC GGGCATCCAA
61       GCAGCAAGCG CGTTACGCCG TGGGTCGATG TTTGATGTTA TGGAGCAGCA ACGATGTTAC
121      GCAGCAGGGC AGTCGCCCTA AAACAAAGTT AAACATCATG GAGAAAAAAA TCACTGGATA
181      TACCACCGTT GATATATCCC AATGGCATCG TAAAGAACAT TTTGAGGCAT TTCAGTCAGT
241      TGCTCAATGT ACCTATAACC AGACCGTTCA GCTGGATATT ACGGCCTTTT TAAAGACCGT
301      AAAGAAAAGT AAGCACAAGT TTTATCCGGC CTTTATTCAC ATTCTTGCCC GCCTGATGAA
361      TGCTCATCCG GAGTTCCGTA TGGCAATGAA AGACGGTGAG CTGGTGATAT GGGATAGTGT
421      TCACCCTTGT TACACCGTTT TCCATGAGCA AACTGAAACG TTTTCATCGC TCTGGAGTGA
481      ATACCACGAC GATTTCCGGC AGTTTCTACA CATATATTCG CAAGATGTGG CGTGTTACGG
541      TGAAAACCTG GCCTATTTCC CTAAAGGGTT TATTGAGAAT ATGTTTTTCG TCTCAGCCAA
601      TCCCTGGGTG AGTTTCACCA GTTTTGATTT AAACGTGGCC AATATGGACA ACTTCTTCGC
661      CCCCGTTTTC ACAATGGGCA AATATTATAC GCAAGGCGAC AAGGTGCTGA TGCCGCTGGC
721      GATTCAGGTT CATCATGCCG TTTGTGATGG CTTCCATGTC GGCAGAATGC TTAATGAATT
781      ACAACAGTAC TGCGATGAGT GGCAGGGCGG GGCGTAATTT TTTTAAGGCA GTTATTGGTG
841      CGATCCTCTA GCTAGAAATT CGTTCAAGCC GACGCCGCTT CGCGGCGCGG CTTAACTCAA
901      GCGTTAGATG CACTAAGCAC ATAATTGCTC ACAGCCAAAC TATCAGGTCA AGTCCCGCGG
961      AGTTGTTCGG TAAATTGTCA CAACGCCGCC CGCCCCGTTC CACTGAGCGT CAGACCCGGT
1021     AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
1081     AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
1141     TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA
1201     GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
1261     AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
1321     AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
1381     GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA
1441     AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
1501     AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
1561     CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
1621     CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
1681     TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCTAG
1741     GATGGATCTC GGGCACGTCT AACTACTAAG CGAGAGTAGG GAACTGCCAG GCATCAAATA
1801     AAACGAAAGG CTCAGTCGGA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC
1861     GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGTGAA GCAACGGCCC
1921     GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAACTAA GCAGAAGGCC
1981     ATCCTGACGG ATGGCCTTTT TGCGTTTCTA CAAACTCTTC CTGTTAGTTA GTTACTTAAG
2041     TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA
2101     CTAAACCGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC
2161     TATTTAGGTG ACACTATAGA ATACTCAAGC TATGCAAGGC CCTTAAGGCC AAGGGGCTTC
2221     GTGCCAACCA ACGAGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT
2281     CAACTTGAAA AAGTGGCACC GAGTCGGTGC TTTTTTTTTT CGTTTTGCAT TGAGTTTTCT
2341     CCGTCGCATG TTTGCAGTTT TATTTTCCGT TTTGCATTGA AATTTCTCCG TCTCATGTTT
2401     GCAGCGTGTT CAAAAAGTAC GCAGCTGTAT TTCACTTATT TACGGCGCCA CATTTTCATG
2461     CCGTTTGTGC CAACTATCCC GAGCTAGTGA ATACAGCTTG GCTTCACACA ACACTGGTGA
2521     CCCGCTGACC TGCTCGTACC TCGTACCGTC GTACGGCACA GCATTTGGAA TTAAAGGGTG
2581     TGATCGATAC TGCTTGCTGC TCATGAATCC AAACCACACG GAGTTCAAAT TCCCACAGAT
2641     TAAGGCTCGT CCGTCGCACA AGGTAATGTG TGAATATTAT ATCTGTCGTG CAAAATTGCC
2701     TGGCCTGCAC AATTGCTGTT ATAGTTGGCG GCAGGGAGAG TTTTAACATT GACTAGCGTG
2761     CTGATAATTT GTGAGAAATA ATAATTGACA AGTAGATACT GACATTTGAG AAGAGCTTCT
2821     GAACTGTTAT TAGTAACAAA AATGGAAAGC TGATGCACGG AAAAAGGAAA GAAAAAGCCA
2881     TACTTTTTTT TAGGTAGGAA AAGAAAAAGC CATACGAGAC TGATGTCTCT CAGATGGGCC
2941     GGGATCTGTC TATCTAGCAG GCAGCAGCCC ACCAACCTCA CGGGCCAGCA ATTACGAGTC
3001     CTTCTAAAAG CTCCCGCCGA GGGGCGCTGG CGCTGCTGTG CAGCAGCACG TCTAACATTA
3061     GTCCCACCTC GCCAGTTTAC AGGGAGCAGA ACCAGCTTAT AAGCGGAGGC GCGGCACCAA
3121     GAAGCGTGAC CAGGAGGAGC ATGCCCCTTT GGCCTATTTG GCCTGGCCCA ATTCGCCCTA
3181     TAGTGAGTCG TATTACAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC
3241     TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG
3301     CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTATACG TACGGCAGTT
3361     TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG TTTGTGGATG TACAGAGTGA
3421     TATTATTGAC ACGCCGGGGC GACGGATGGT GATCCCCCTG GCCAGTGCAC GTCTGCTGTC
3481     AGATAAAGTC TCCCGTGAAC TTTACCCGGT GGTGCATATC GGGGATGAAA GCTGGCGCAT
3541     GATGACCACC GATATGGCAA GTGTGCCAGT ATCCGTTATC GGGGAAGAAG TGGCTGATCT
3601     CAGCCACCGC GAAAATGACA TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT
3661     GTCAGGCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTCACGT AGAAAGCCAG
3721     TCCGCAGAAA CGGACCATGA GTGGACATAA GCCTCGTTCG GTTCGTAAGC TGTAATGCAA
3781     GTAGCGTATG CGCTCACGCA ACTGGTCCAG AACCTTGACC GAACGCAGCG GTGGTAACGG
3841     CGCAGTGGC