测序及更多信息： https://pan.baidu.com/s/1-K4VST2u8v9Db0g3JvKTEw 
提取码：21ua 

采购地址：淘宝店铺地址

我们的官网  http://hedgehogbio.comhttp://hedgehogbio.com/

pIB/V5-His 昆虫表达载体

载体基本信息

载体名称:	pIB/V5-His
质粒类型:	昆虫表达载体
克隆方法:	多克隆位点，限制性内切酶
启动子:	OplE2
载体大小:	3521 bp
5' 测序引物及序列:	OpIE2 Forward：CGCAACGATCTGGTAAACAC
3' 测序引物及序列:	5'd[CGCAACGATCTGGTAAACAC]3'
载体标签:	V5 Epitope Tag , His Tag (C-末端)
载体抗性:	氨苄青霉素
真核筛选标记:	Blasticidin
克隆菌株:	TOP10、DH5
宿主细胞（系）:	Sf9 、High Five
瞬表达/稳表达:	瞬表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

 

 

 

 

 

         The InsectSelect BSD Kit features the pIB/V5-His vector for stable expression of your gene of interest in a variety of common insect cells. This vector has several features that facilitate

expression, analysis, and detection of recombinant protein in insect cells:

• The OpIE2 promoter for constitutive expression

• The Blasticidin resistance gene for rapid selection of stably transfected cells in two weeks

• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin

The InsectSelect BSD Kit features the pIB/V5-His vector for stable expression of your gene of interest in a variety of common insect cells. This vector has several features that facilitate 

expression, analysis, and detection of recombinant protein in insect cells:

• The OpIE2 promoter for constitutive expression
• The Blasticidin resistance gene for rapid selection of stably transfected cells in two weeks

• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin

全序列

1        CATGATGATA AACAATGTAT GGTGCTAATG TTGCTTCAAC AACAATTCTG TTGAACTGTG
61       TTTTCATGTT TGCCAACAAG CACCTTTATA CTCGGTGGCC TCCCCACCAC CAACTTTTTT
121      GCACTGCAAA AAAACACGCT TTTGCACGCG GGCCCATACA TAGTACAAAC TCTACGTTTC
181      GTAGACTATT TTACATAAAT AGTCTACACC GTTGTATACG CTCCAAATAC ACTACCACAC
241      ATTGAACCTT TTTGCAGTGC AAAAAAGTAC GTGTCGGCAG TCACGTAGGC CGGCCTTATC
301      GGGTCGCGTC CTGTCACGTA CGAATCACAT TATCGGACCG GACGAGTGTT GTCTTATCGT
361      GACAGGACGC CAGCTTCCTG TGTTGCTAAC CGCAGCCGGA CGCAACTCCT TATCGGAACA
421      GGACGCGCCT CCATATCAGC CGCGCGTTAT CTCATGCGCG TGACCGGACA CGAGGCGCCC
481      GTCCCGCTTA TCGCGCCTAT AAATACAGCC CGCAACGATC TGGTAAACAC AGTTGAACAG
541      CATCTGTTCG AATTTAAAGC TTGGTACCGA GCTCGGATCC ACTAGTCCAG TGTGGTGGAA
601      TTCTGCAGAT ATCCAGCACA GTGGCGGCCG CTCGAGTCTA GAGGGCCCGC GGTTCGAAGG
661      TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG CGTACCGGTC ATCATCACCA
721      TCACCATTGA GTTTATCTGA CTAAATCTTA GTTTGTATTG TCATGTTTTA ATACAATATG
781      TTATGTTTAA ATATGTTTTT AATAAATTTT ATAAAATAAT TTCAACTTTT ATTGTAACAA
841      CATTGTCCAT TTACACACTC CTTTCAAGCG CGTGGGATCG ATGCTCACTC AAAGGCGGTA
901      ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
961      CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
1021     CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
1081     TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
1141     CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
1201     TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
1261     GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
1321     CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
1381     AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
1441     AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
1501     AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
1561     CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
1621     GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGCGAAA CACGCACGGC
1681     GCGCGCACGC AGCTTAGCAC AAACGCGTCG TTGCACGCGC CCACCGCTAA CCGCAGGCCA
1741     ATCGGTCGGC CGGCCTCATA TCCGCTCACC AGCCGCGTCC TATCGGGCGC GGCTTCCGCG
1801     CCCATTTTGA ATAAATAAAC GATAACGCCG TTGGTGGCGT GAGGCATGTA AAAGGTTACA
1861     TCATTATCTT GTTCGCCATC CGGTTGGTAT AAATAGACGT TCATGTTGGT TTTTGTTTCA
1921     GTTGCAAGTT GGCTGCGGCG CGCGCAGCAC CTTTGCCGGG ATCTGCCGGG CTGCAGCACG
1981     TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA
2041     CTAAACCATG GCCAAGCCTT TGTCTCAAGA AGAATCCACC CTCATTGAAA GAGCAACGGC
2101     TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC GCCAGCGCAG CTCTCTCTAG
2161     CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT ACTGGGGGAC CTTGCGCAGA
2221     ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC AACCTGACTT GTATCGTCGC
2281     GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA CGGTGCCGAC AGGTTCTTCT
2341     CGATCTGCAT CCTGGGATCA AAGCCATAGT GAAGGACAGT GATGGACAGC CGACGGCAGT
2401     TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG GGCTAAGCAC TTCGTGGCCG
2461     AGGAGCAGGA CTGACACGTC CCGGGAGATC TGCATGTCTA CTAAACTCAC AAATTAGAGC
2521     TTCAATTTAA TTATATCAGT TATTACCCAT TGAAAAAGGA AGAGTATGAG TATTCAACAT
2581     TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA
2641     GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC
2701     GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA
2761     ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG
2821     CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA
2881     GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA
2941     ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG
3001     CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG
3061     GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA
3121     ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA
3181     ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT
3241     GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA
3301     GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG
3361     GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT
3421     TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT
3481     TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC T