测序及更多信息： https://pan.baidu.com/s/1M9uwq6nDRcI_GICCg6jY5g 

提取码：12bq 

采购地址：淘宝店铺地址

我们的官网  http://hedgehogbio.comhttp://hedgehogbio.com/

pC001 - huLshC2C2-MBP for bacterial expression 
(Plasmid #79150)

 

载体作用

原核表达人源密码子优化的LshC2c2（Cas13a）质粒

Expresses human codon-optimized LshC2c2 for purification in E. coli

载体骨架

BPV00356 pET21GG2-His(6)-MBP_Flag-Avi

构建方法

载体类型

原核表达， CRISPR

载体抗性

Ampicillin

筛选标记

-

插入基因信息

C2c2

参考文献

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F. Science. 2016 Jun 2. pii: aaf5573. 10.1126/science.aaf5573 PubMed 27256883

载体图谱↑蓝色为测序比对区

全序列

1        TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
61       CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG
121      GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG
181      TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC
241      TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG
301      CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG
361      CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG
421      AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA
481      ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC
541      GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA
601      GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG
661      GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC
721      CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC
781      CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG
841      ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG
901      GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA
961      AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC
1021     TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG
1081     GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC
1141     GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA
1201     GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA
1261     CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC
1321     GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG
1381     TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT
1441     ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC CCTCTAGAAA TAATTTTGTT
1501     TAACTTTAAG AAGGAGATAT ACATATGGGT CATCATCATC ACCATCACAA AATCGAAGAA
1561     GGCAAACTGG TTATTTGGAT CAATGGCGAT AAAGGCTATA ATGGTCTGGC AGAAGTTGGC
1621     AAAAAATTCG AAAAAGATAC CGGCATTAAA GTGACCGTTG AACATCCGGA TAAACTGGAA
1681     GAAAAATTTC CGCAGGTTGC AGCAACCGGT GATGGTCCGG ATATTATCTT TTGGGCACAT
1741     GATCGTTTTG GTGGTTATGC ACAGAGCGGT CTGCTGGCAG AAATTACACC GGATAAAGCA
1801     TTTCAGGACA AACTGTATCC GTTTACCTGG GATGCAGTTC GCTATAACGG TAAACTGATT
1861     GCATATCCGA TTGCAGTTGA AGCACTGAGC CTGATCTATA ACAAAGATCT GCTGCCGAAT
1921     CCGCCTAAAA CCTGGGAAGA AATTCCGGCA CTGGATAAAG AACTGAAAGC AAAAGGTAAA
1981     AGCGCACTGA TGTTTAATCT GCAAGAACCG TATTTTACCT GGCCTCTGAT TGCAGCAGAT
2041     GGTGGCTATG CATTCAAATA TGAAAACGGC AAATATGATA TCAAAGACGT GGGTGTTGAT
2101     AATGCGGGTG CAAAAGCCGG TCTGACCTTT CTGGTTGATC TGATTAAAAA CAAACACATG
2161     AACGCCGATA CCGATTATAG CATTGCAGAA GCAGCATTTA ACAAAGGTGA AACCGCAATG
2221     ACAATTAATG GTCCGTGGGC ATGGTCAAAT ATTGATACCA GCAAAGTGAA TTATGGTGTT
2281     ACCGTTCTGC CGACATTTAA AGGTCAGCCG AGCAAACCGT TTGTTGGTGT TCTGAGCGCA
2341     GGTATTAATG CAGCAAGCCC GAACAAAGAA CTGGCAAAAG AATTTCTGGA AAACTATCTG
2401     CTGACCGATG AAGGTCTGGA AGCAGTGAAT AAAGATAAAC CGCTGGGTGC AGTTGCACTG
2461     AAAAGCTATG AAGAAGAACT GGTTAAAGAT CCGCGTATTG CAGCCACCAT GGAAAATGCA
2521     CAGAAAGGCG AAATTATGCC GAATATTCCG CAGATGAGCG CATTTTGGTA TGCCGTTCGT
2581     ACCGCAGTGA TTAATGCCGC ATCAGGTCGT CAGACCGTTG ATGAAGCCCT GAAAGATGCC
2641     CAGACCGGTG CAAGCACCGG ATCCCTGGTT CCGCGTGGTA GCGCTAGCGA AAATCTGTAT
2701     TTTCAGGGAA TGGGGAACCT GTTCGGACAC AAGCGGTGGT ACGAGGTGCG AGATAAGAAA
2761     GACTTCAAGA TCAAGCGAAA GGTGAAAGTC AAGCGGAATT ATGACGGGAA CAAGTACATT
2821     CTGAACATCA ACGAAAACAA CAACAAAGAG AAGATCGACA ACAACAAGTT CATCAGAAAG
2881     TACATCAACT ACAAGAAAAA CGATAATATT CTGAAGGAGT TCACTAGGAA ATTTCATGCA
2941     GGAAATATCC TGTTCAAACT GAAGGGCAAA GAAGGGATCA TTAGAATTGA GAATAACGAC
3001     GACTTCCTGG AGACAGAGGA AGTGGTGCTG TATATCGAGG CCTACGGCAA GAGCGAGAAG
3061     CTGAAAGCAC TGGGGATCAC AAAGAAAAAG ATCATTGACG AGGCCATCAG GCAGGGCATT
3121     ACTAAGGACG ATAAAAAGAT CGAGATCAAG CGACAGGAGA ACGAGGAAGA GATCGAAATT
3181     GACATCCGGG ATGAGTACAC TAATAAGACC CTGAACGACT GCTCCATCAT TCTGCGCATC
3241     ATTGAAAACG ATGAACTGGA GACAAAGAAG AGCATCTACG AGATCTTCAA GAACATCAAT
3301     ATGAGCCTGT ATAAGATCAT CGAGAAGATC ATCGAAAACG AGACAGAAAA GGTGTTTGAA
3361     AATAGATACT ACGAAGAGCA CCTGAGGGAG AAGCTGCTGA AAGACGATAA GATTGACGTG
3421     ATCCTGACCA ACTTCATGGA AATCCGGGAG AAGATCAAGT CTAATCTGGA GATCCTGGGC
3481     TTCGTGAAGT TTTACCTGAA CGTCGGCGGG GACAAAAAGA AAAGTAAAAA TAAGAAAATG
3541     CTGGTGGAAA AGATTCTGAA CATCAATGTG GATCTGACCG TCGAGGACAT TGCCGATTTC
3601     GTGATCAAGG AGCTGGAATT TTGGAACATC ACAAAGCGCA TTGAGAAAGT GAAGAAAGTC
3661     AATAACGAGT TCCTGGAGAA GCGGAGAAAT CGGACATACA TCAAGTCCTA TGTGCTGCTG
3721     GACAAGCACG AAAAGTTTAA AATCGAGAGA GAAAACAAGA AGGATAAGAT CGTGAAGTTC
3781     TTTGTCGAGA ACATTAAGAA CAACTCTATC AAGGAAAAGA TTGAGAAGAT CCTGGCTGAG
3841     TTCAAGATCG ACGAGCTGAT TAAGAAACTG GAGAAGGAAC TGAAGAAAGG GAACTGTGAT
3901     ACCGAGATCT TCGGAATCTT TAAGAAGCAT TACAAGGTGA ACTTCGACAG CAAGAAATTT
3961     TCCAAGAAAT CTGATGAAGA GAAGGAGCTG TATAAGATCA TCTACAGATA CCTGAAGGGC
4021     AGAATTGAAA AAATCCTGGT GAACGAGCAG AAGGTCAGAC TGAAGAAAAT GGAGAAGATC
4081     GAGATCGAAA AGATTCTGAA TGAAAGTATC CTGTCAGAGA AAATTCTGAA GAGAGTGAAA
4141     CAGTATACAC TGGAGCACAT TATGTACCTG GGGAAGCTGA GGCATAACGA CATCGATATG
4201     ACCACAGTGA ATACTGACGA TTTCAGCCGC CTGCACGCCA AGGAAGAGCT GGACCTGGAA
4261     CTGATCACCT TCTTTGCCAG CACAAATATG GAGCTGAACA AGATCTTTTC CCGAGAAAAC
4321     ATCAACAACG ACGAGAACAT CGATTTCTTT GGAGGCGACC GGGAGAAGAA CTATGTGCTG
4381     GATAAGAAAA TCCTGAATAG TAAGATCAAG ATCATCCGCG ACCTGGATTT CATCGATAAC
4441     AAGAACAACA TCACAAACAA CTTCATTCGA AAGTTTACAA AGATCGGCAC TAATGAAAGG
4501     AACCGCATCC TGCATGCCAT TTCCAAAGAG AGGGACCTGC AGGGGACTCA GGACGATTAC
4561     AACAAAGTGA TCAACATCAT TCAGAATCTG AAGATCTCCG ATGAAGAGGT GAGCAAAGCT
4621     CTGAACCTGG ACGTGGTCTT TAAGGACAAG AAAAACATCA TCACAAAGAT CAATGACATC
4681     AAGATCTCTG AAGAGAACAA CAACGATATC AAGTATCTGC CCAGCTTCAG CAAAGTGCTG
4741     CCCGAAATCC TGAACCTGTA CCGCAACAAT CCCAAGAATG AGCCTTTTGA CACAATCGAG
4801     ACTGAAAAAA TTGTGCTGAA CGCTCTGATC TACGTCAATA AGGAGCTGTA TAAGAAACTG
4861     ATCCTGGAGG ACGATCTGGA AGAGAACGAG TCCAAGAATA TCTTCCTGCA GGAACTGAAG
4921     AAAACCCTGG GCAACATTGA CGAAATCGAT GAGAACATCA TCGAGAACTA CTACAAGAAC
4981     GCACAGATTT CTGCCAGTAA GGGGAACAAC AAGGCAATCA AGAAATATCA GAAGAAAGTG
5041     ATCGAGTGCT ACATTGGATA TCTGCGCAAA AACTACGAAG AGCTGTTCGA CTTTTCAGAC
5101     TTCAAGATGA ACATCCAGGA AATCAAGAAA CAGATTAAGG ACATCAACGA TAACAAGACT
5161     TATGAGCGGA TCACCGTGAA AACCAGCGAC AAAACCATTG TCATCAACGA CGATTTCGAG
5221     TACATCATTT CTATCTTTGC ACTGCTGAAC AGTAATGCCG TGATTAATAA GATCCGAAAC
5281     AGATTCTTCG CCACCAGCGT GTGGCTGAAC ACCTCAGAAT ACCAGAATAT CATTGACATC
5341     CTGGATGAGA TTATGCAGCT GAATACCCTG CGGAACGAAT GCATCACAGA GAACTGGAAT
5401     CTGAACCTGG AAGAGTTCAT TCAGAAGATG AAAGAGATCG AAAAGGATTT CGACGACTTC
5461     AAGATCCAGA CTAAGAAAGA AATCTTCAAC AACTACTACG AGGACATCAA GAACAACATT
5521     CTGACCGAGT TTAAAGACGA TATCAACGGC TGTGATGTGC TGGAAAAGAA ACTGGAGAAG
5581     ATTGTCATCT TCGACGATGA AACCAAGTTC GAGATCGACA AGAAATCCAA CATCCTGCAG
5641     GATGAACAGA GAAAGCTGTC TAACATCAAC AAGAAGGACC TGAAGAAAAA GGTGGATCAG
5701     TATATCAAGG ACAAAGATCA GGAGATCAAG TCTAAAATCC TGTGCAGGAT CATTTTCAAC
5761     AGTGACTTTC TGAAAAAGTA CAAGAAGGAA ATCGACAATC TGATTGAGGA TATGGAGTCT
5821     GAAAATGAGA ACAAGTTCCA GGAGATCTAC TATCCCAAGG AACGGAAGAA CGAGCTGTAT
5881     ATCTACAAAA AGAATCTGTT CCTGAACATC GGAAATCCTA ACTTTGACAA GATCTACGGC
5941     CTGATTAGCA ACGACATCAA GATGGCCGAT GCTAAATTCC TGTTTAATAT CGATGGAAAG
6001     AACATCAGAA AGAACAAAAT CAGTGAGATC GACGCTATTC TGAAGAATCT GAACGATAAA
6061     CTGAACGGCT ACTCAAAGGA ATACAAGGAG AAGTACATCA AAAAGCTGAA GGAGAACGAC
6121     GATTTCTTTG CAAAGAACAT CCAGAATAAG AACTACAAAT CCTTCGAAAA GGACTATAAC
6181     CGCGTGTCTG AGTACAAAAA GATTCGAGAT CTGGTCGAGT TCAACTATCT GAACAAAATC
6241     GAGTCCTACC TGATTGACAT CAACTGGAAG CTGGCTATTC AGATGGCAAG ATTCGAAAGG
6301     GATATGCACT ATATCGTGAA TGGACTGAGG GAGCTGGGCA TCATTAAGCT GTCAGGCTAT
6361     AACACCGGGA TCAGCAGGGC ATACCCAAAG CGCAATGGAA GCGACGGCTT TTACACTACC
6421     ACAGCCTACT ACAAGTTCTT TGATGAAGAG TCCTACAAGA AGTTCGAGAA GATTTGCTAC
6481     GGGTTTGGAA TCGACCTGAG CGAAAATTCC GAGATCAACA AGCCTGAAAA TGAGAGCATT
6541     CGGAACTATA TCTCCCATTT CTACATCGTG AGAAATCCAT TTGCCGACTA CAGTATTGCT
6601     GAGCAGATCG ATCGGGTGAG CAACCTGCTG TCATATAGCA CACGCTACAA CAATTCAACT
6661     TATGCCAGCG TGTTCGAAGT CTTTAAAAAG GACGTGAATC TGGACTACGA TGAGCTGAAA
6721     AAGAAATTCA AACTGATCGG CAACAATGAT ATTCTGGAGC GCCTGATGAA GCCCAAGAAA
6781     GTGAGCGTCC TGGAACTGGA GTCCTACAAC AGTGACTACA TTAAGAATCT GATCATTGAA
6841     CTGCTGACCA AAATCGAGAA TACTAACGAT ACCCTGAAAA GGCCGGCGGC CACGAAAAAG
6901     GCCGGCCAGG CAAAAAAGAA AAAGGGATCC TACCCATACG ATGTTCCAGA TTACGCTTAT
6961     CCCTACGACG TGCCTGATTA TGCATACCCA TATGATGTCC CCGACTATGC CTAAGCCTCG
7021     AGGGTGGTGG TAGCGATTAT AAAGATGATG ATGATAAAGG CCTGAACGAT ATCTTTGAAG
7081     CCCAGAAAAT TGAATGGCAC GAGTAAAAGC TTGTCGAGCA CCACCACCAC CACCACTGAG
7141     ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT
7201     AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG
7261     GAACTATATC CGGATTGGCG AATGGGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG
7321     TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT
7381     CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG
7441     GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA
7501     TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC
7561     GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC
7621     TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA
7681     AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT
7741     TTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
7801     ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
7861     AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
7921     ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
7981     TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
8041     GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
8101     CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
8161     TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
8221     AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT
8281     TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA
8341     TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG
8401     TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT
8461     ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG
8521     ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG
8581     TGAGCGTGGT TCACGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT
8641     CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC
8701     TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT
8761     ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT
8821     TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC
8881     CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
8941     GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
9001     TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT
9061     GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
9121     GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
9181     CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
9241     ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
9301     AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
9361     CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
9421     TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
9481     GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC
9541     TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC
9601     CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG
9661     CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC
9721     ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG
9781     TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC
9841     CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA
9901     CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC
9961     AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT
10021    CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG
10081    CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC
10141    TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG
10201    ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC
10261    GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG
10321    GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG
10381    GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG
10441    TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG
10501    ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA
10561    GCACGATCAT GCGCACCCGT GGGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA
10621    AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA
10681    CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA
10741    CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG
10801    CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA
10861    AGGGCATCGG TCGAGATCCC GGTGCCTAAT GAGTGAGCTA ACTTACATTA ATTGCGTTGC
10921    GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCG