测序及更多信息https://pan.baidu.com/s/1Q46GvhS0l8D1h4rge8WZBA
提取码：ld2f
采购地址：淘宝店铺地址
我们的官网 http://hedgehogbio.comhttp://hedgehogbio.com/
载体基本信息
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载体名称:
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pET-NLS-Cas9-6xHis
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质粒类型:
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CRISPR/Cas9载体；细菌基因敲除载体
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高拷贝/低拷贝:
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高拷贝
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插入位点:
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限制性内切酶，多克隆位点
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启动子:
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lac/T7
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载体大小:
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9289
bp
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5' 测序引物及序列:
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T7
forward：5’TAATACGACTCACTATAGGG 3’
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3' 测序引物及序列:
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T7
Terminal：5’GCTAGTTATTGCTCAGCGG 3’
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载体标签:
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NLS (N
ter); His tag (C-ter）
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载体抗性:
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Ampicillin
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宿主细胞（系）:
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细菌，如大肠杆菌
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产品目录号:
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#62934
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稳定性:
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稳表达
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组成型/诱导型:
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诱导型
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全序列
1 CGGGATCTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT
61 TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA
121 GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA
181 AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC
241 CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCGATCTCG
301 ATCCCGCGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC AATTCCCCTC
361 TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGCCCAAGAA GAAGAGGAAG
421 GTGATGGATA AGAAATACTC AATAGGCTTA GATATCGGCA CAAATAGCGT CGGATGGGCG
481 GTGATCACTG ATGAATATAA GGTTCCGTCT AAAAAGTTCA AGGTTCTGGG AAATACAGAC
541 CGCCACAGTA TCAAAAAAAA TCTTATAGGG GCTCTTTTAT TTGACAGTGG AGAGACAGCG
601 GAAGCGACTC GTCTCAAACG GACAGCTCGT AGAAGGTATA CACGTCGGAA GAATCGTATT
661 TGTTATCTAC AGGAGATTTT TTCAAATGAG ATGGCGAAAG TAGATGATAG TTTCTTTCAT
721 CGACTTGAAG AGTCTTTTTT GGTGGAAGAA GACAAGAAGC ATGAACGTCA TCCTATTTTT
781 GGAAATATAG TAGATGAAGT TGCTTATCAT GAGAAATATC CAACTATCTA TCATCTGCGA
841 AAAAAATTGG TAGATTCTAC TGATAAAGCG GATTTGCGCT TAATCTATTT GGCCTTAGCG
901 CATATGATTA AGTTTCGTGG TCATTTTTTG ATTGAGGGAG ATTTAAATCC TGATAATAGT
961 GATGTGGACA AACTATTTAT CCAGTTGGTA CAAACCTACA ATCAATTATT TGAAGAAAAC
1021 CCTATTAACG CAAGTGGAGT AGATGCTAAA GCGATTCTTT CTGCACGATT GAGTAAATCA
1081 AGACGATTAG AAAATCTCAT TGCTCAGCTC CCCGGTGAGA AGAAAAATGG CTTATTTGGG
1141 AATCTCATTG CTTTGTCATT GGGTTTGACC CCTAATTTTA AATCAAATTT TGATTTGGCA
1201 GAAGATGCTA AATTACAGCT TTCAAAAGAT ACTTACGATG ATGATTTAGA TAATTTATTG
1261 GCGCAAATTG GAGATCAATA TGCTGATTTG TTTTTGGCAG CTAAGAATTT ATCAGATGCT
1321 ATTTTACTTT CAGATATCCT AAGAGTAAAT ACTGAAATAA CTAAGGCTCC CCTATCAGCT
1381 TCAATGATTA AACGCTACGA TGAACATCAT CAAGACTTGA CTCTTTTAAA AGCTTTAGTT
1441 CGACAACAAC TTCCAGAAAA GTATAAAGAA ATCTTTTTTG ATCAATCAAA AAACGGATAT
1501 GCAGGTTATA TTGATGGGGG AGCTAGCCAA GAAGAATTTT ATAAATTTAT CAAACCAATT
1561 TTAGAAAAAA TGGATGGTAC TGAGGAATTA TTGGTGAAAC TAAATCGTGA AGATTTGCTG
1621 CGCAAGCAAC GGACCTTTGA CAACGGCTCT ATTCCCCATC AAATTCACTT GGGTGAGCTG
1681 CATGCTATTT TGAGAAGACA AGAAGACTTT TATCCATTTT TAAAAGACAA TCGTGAGAAG
1741 ATTGAAAAAA TCTTGACTTT TCGAATTCCT TATTATGTTG GTCCATTGGC GCGTGGCAAT
1801 AGTCGTTTTG CATGGATGAC TCGGAAGTCT GAAGAAACAA TTACCCCATG GAATTTTGAA
1861 GAAGTTGTCG ATAAAGGTGC TTCAGCTCAA TCATTTATTG AACGCATGAC AAACTTTGAT
1921 AAAAATCTTC CAAATGAAAA AGTACTACCA AAACATAGTT TGCTTTATGA GTATTTTACG
1981 GTTTATAACG AATTGACAAA GGTCAAATAT GTTACTGAAG GAATGCGAAA ACCAGCATTT
2041 CTTTCAGGTG AACAGAAGAA AGCCATTGTT GATTTACTCT TCAAAACAAA TCGAAAAGTA
2101 ACCGTTAAGC AATTAAAAGA AGATTATTTC AAAAAAATAG AATGTTTTGA TAGTGTTGAA
2161 ATTTCAGGAG TTGAAGATAG ATTTAATGCT TCATTAGGTA CCTACCATGA TTTGCTAAAA
2221 ATTATTAAAG ATAAAGATTT TTTGGATAAT GAAGAAAATG AAGATATCTT AGAGGATATT
2281 GTTTTAACAT TGACCTTATT TGAAGATAGG GAGATGATTG AGGAAAGACT TAAAACATAT
2341 GCTCACCTCT TTGATGATAA GGTGATGAAA CAGCTTAAAC GTCGCCGTTA TACTGGTTGG
2401 GGACGTTTGT CTCGAAAATT GATTAATGGT ATTAGGGATA AGCAATCTGG CAAAACAATA
2461 TTAGATTTTT TGAAATCAGA TGGTTTTGCC AATCGCAATT TTATGCAGCT GATCCATGAT
2521 GATAGTTTGA CATTTAAAGA AGACATTCAA AAAGCACAAG TGTCTGGACA AGGCGATAGT
2581 TTACATGAAC ATATTGCAAA TTTAGCTGGT AGCCCTGCTA TTAAAAAAGG TATTTTACAG
2641 ACTGTAAAAG TTGTTGATGA ATTGGTCAAA GTAATGGGGC GGCATAAGCC AGAAAATATC
2701 GTTATTGAAA TGGCACGTGA AAATCAGACA ACTCAAAAGG GCCAGAAAAA TTCGCGAGAG
2761 CGTATGAAAC GAATCGAAGA AGGTATCAAA GAATTAGGAA GTCAGATTCT TAAAGAGCAT
2821 CCTGTTGAAA ATACTCAATT GCAAAATGAA AAGCTCTATC TCTATTATCT CCAAAATGGA
2881 AGAGACATGT ATGTGGACCA AGAATTAGAT ATTAATCGTT TAAGTGATTA TGATGTCGAT
2941 CACATTGTTC CACAAAGTTT CCTTAAAGAC GATTCAATAG ACAATAAGGT CTTAACGCGT
3001 TCTGATAAAA ATCGTGGTAA ATCGGATAAC GTTCCAAGTG AAGAAGTAGT CAAAAAGATG
3061 AAAAACTATT GGAGACAACT TCTAAACGCC AAGTTAATCA CTCAACGTAA GTTTGATAAT
3121 TTAACGAAAG CTGAACGTGG AGGTTTGAGT GAACTTGATA AAGCTGGTTT TATCAAACGC
3181 CAATTGGTTG AAACTCGCCA AATCACTAAG CATGTGGCAC AAATTTTGGA TAGTCGCATG
3241 AATACTAAAT ACGATGAAAA TGATAAACTT ATTCGAGAGG TTAAAGTGAT TACCTTAAAA
3301 TCTAAATTAG TTTCTGACTT CCGAAAAGAT TTCCAATTCT ATAAAGTACG TGAGATTAAC
3361 AATTACCATC ATGCCCATGA TGCGTATCTA AATGCCGTCG TTGGAACTGC TTTGATTAAG
3421 AAATATCCAA AACTTGAATC GGAGTTTGTC TATGGTGATT ATAAAGTTTA TGATGTTCGT
3481 AAAATGATTG CTAAGTCTGA GCAAGAAATA GGCAAAGCAA CCGCAAAATA TTTCTTTTAC
3541 TCTAATATCA TGAACTTCTT CAAAACAGAA ATTACACTTG CAAATGGAGA GATTCGCAAA
3601 CGCCCTCTAA TCGAAACTAA TGGGGAAACT GGAGAAATTG TCTGGGATAA AGGGCGAGAT
3661 TTTGCCACAG TGCGCAAAGT ATTGTCCATG CCCCAAGTCA ATATTGTCAA GAAAACAGAA
3721 GTACAGACAG GCGGATTCTC CAAGGAGTCA ATTTTACCAA AAAGAAATTC GGACAAGCTT
3781 ATTGCTCGTA AAAAAGACTG GGATCCAAAA AAATATGGTG GTTTTGATAG TCCAACGGTA
3841 GCTTATTCAG TCCTAGTGGT TGCTAAGGTG GAAAAAGGGA AATCGAAGAA GTTAAAATCC
3901 GTTAAAGAGT TACTAGGGAT CACAATTATG GAAAGAAGTT CCTTTGAAAA AAATCCGATT
3961 GACTTTTTAG AAGCTAAAGG ATATAAGGAA GTTAAAAAAG ACTTAATCAT TAAACTACCT
4021 AAATATAGTC TTTTTGAGTT AGAAAACGGT CGTAAACGGA TGCTGGCTAG TGCCGGAGAA
4081 TTACAAAAAG GAAATGAGCT GGCTCTGCCA AGCAAATATG TGAATTTTTT ATATTTAGCT
4141 AGTCATTATG AAAAGTTGAA GGGTAGTCCA GAAGATAACG AACAAAAACA ATTGTTTGTT
4201 GAGCAGCATA AGCATTATTT AGATGAGATT ATTGAGCAAA TCAGTGAATT TTCTAAGCGT
4261 GTTATTTTAG CAGATGCCAA TTTAGATAAA GTTCTTAGTG CATATAACAA ACATAGAGAC
4321 AAACCAATAC GTGAACAAGC AGAAAATATT ATTCATTTAT TTACGTTGAC GAATCTTGGA
4381 GCTCCCGCTG CTTTTAAATA TTTTGATACA ACAATTGATC GTAAACGATA TACGTCTACA
4441 AAAGAAGTTT TAGATGCCAC TCTTATCCAT CAATCCATCA CTGGTCTTTA TGAAACACGC
4501 ATTGATTTGA GTCAGCTAGG AGGTGACCAT CACCACCACC ATCACTAATG ACTCGAGTCT
4561 GGTAAAGAAA CCGCTGCTGC GAAATTTGAA CGCCAGCACA TGGACTCGTC TACTAGCGCA
4621 GCTTAATTAA CCTAGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
4681 TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATTGGCGAAT
4741 GGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA
4801 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG
4861 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT
4921 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG
4981 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA
5041 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT
5101 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT
5161 TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTC TGGCGGCACG ATGGCATGAG
5221 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT
5281 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC
5341 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
5401 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC
5461 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
5521 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG
5581 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT
5641 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
5701 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT
5761 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC
5821 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC
5881 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA
5941 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
6001 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC
6061 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG
6121 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT
6181 CCTTTTTCAA TCATGATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
6241 TTGAATGTAT TTAGAAAAAT AAACAAATAG GTCATGACCA AAATCCCTTA ACGTGAGTTT
6301 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT
6361 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT
6421 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG
6481 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA
6541 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT
6601 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG
6661 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG
6721 AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC
6781 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA
6841 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT
6901 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA
6961 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT
7021 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG
7081 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC
7141 CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT
7201 CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC
7261 TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC
7321 ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC
7381 GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA
7441 TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA
7501 TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA
7561 TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG
7621 GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG
7681 TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA
7741 GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA
7801 GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG
7861 GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT
7921 TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC
7981 TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCTAGTC ATGCCCCGCG CCCACCGGAA
8041 GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA GATCCCGGTG CCTAATGAGT
8101 GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
8161 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
8221 CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT
8281 GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT
8341 GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA
8401 CTACCGAGAT GTCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA
8461 GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT
8521 GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT
8581 GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG
8641 AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA
8701 CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG
8761 AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT
8821 GGTCATCCAG CGGATAGTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA AGATTGTGCA
8881 CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC GTTCTACCAT CGACACCACC ACGCTGGCAC
8941 CCAGTTGATC GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGGCCA
9001 GACTGGAGGT GGCAACGCCA ATCAGCAACG ACTGTTTGCC CGCCAGTTGT TGTGCCACGC
9061 GGTTGGGAAT GTAATTCAGC TCCGCCATCG CCGCTTCCAC TTTTTCCCGC GTTTTCGCAG
9121 AAACGTGGCT GGCCTGGTTC ACCACGCGGG AAACGGTCTG ATAAGAGACA CCGGCATACT
9181 CTGCGACATC GTATAACGTT ACTGGTTTCA CATTCACCAC CCTGAATTGA CTCTCTTCCG
9241 GGCGCTATCA TGCCATACCG CGAAAGGTTT TGCGCCATTC GATGGTGTC
