酵母编辑质粒

Cas9-NAT (Plasmid #64329) 酵母基因编辑载体

发布时间:2019-02-13 20:22:28 | 浏览次数：

测序及更多信息：链接：https://pan.baidu.com/s/1e9JRJkKTtZkaESCVLRQ-XA
提取码：1d5l

采购地址：淘宝店铺地址

PURPOSE

Cas9 expression cassette carried by a yeast single-copy episome plasmid

BACKBONE

Vector backbone
pRS414-TEF1p-Cas9-CYC1t
Backbone manufacturer
George Church lab (Addgene Plasmid #43802)
Backbone size w/o insert (bp)9232
Total vector size (bp)10446
Vector type
Yeast Expression, CRISPR
Selectable markers
Nourseothricin(clonNat)

GENE/INSERT

Gene/Insert name
Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
Insert Size (bp)
1206

参考文献

Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS. Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3. 10.1128/AEM.02310-14 PubMed 25281382