• PURPOSE

  Cas9 expression cassette carried by a yeast single-copy episome plasmid

BACKBONE

• Vector backbone
  pRS414-TEF1p-Cas9-CYC1t
• Backbone manufacturer
  George Church lab (Addgene Plasmid #43802)
• Backbone size w/o insert (bp)9232
• Total vector size (bp)10446
• Vector type
  Yeast Expression, CRISPR
• Selectable markers
  Nourseothricin(clonNat)

GENE/INSERT

• Gene/Insert name
  Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
• Insert Size (bp)
  1206

Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS. Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3. 10.1128/AEM.02310-14 PubMed 25281382